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Tissue culture and rapid propagation method of angel red soft-seed pomegranate

A technology of tissue culture and soft seeds, which is applied in the field of plant tissue culture, can solve the problem of the inability to realize the excellent traits of plant tissue culture propagation speed, there is no research report on the tissue culture and rapid propagation of angel red soft seed pomegranate, and there are few varieties of angel red soft seed pomegranate resources and other problems, to achieve good rooting effect, inhibit browning, and grow well

Inactive Publication Date: 2018-12-21
南京榴元生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the precious variety of angel red soft seed pomegranate and few resources, conventional breeding methods cannot meet the current production and market demand, and cannot achieve fast propagation speed of plant tissue culture, maintain the excellent traits of the original variety, and carry out annual factory production, etc. Advantage
At present, there is no research report on tissue culture and rapid propagation of angel red soft seed pomegranate

Method used

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  • Tissue culture and rapid propagation method of angel red soft-seed pomegranate
  • Tissue culture and rapid propagation method of angel red soft-seed pomegranate
  • Tissue culture and rapid propagation method of angel red soft-seed pomegranate

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Embodiment

[0032] A kind of angel red soft seed pomegranate tissue culture rapid propagation method comprises the following steps:

[0033] 1) Collect young stems as explants, sterilize with 75% alcohol for 30 s, then sterilize with 0.1% mercuric chloride for 10 min, and wash thoroughly with sterile water;

[0034] 2) Inoculate the sterilized stem segments on the adventitious bud induction medium with the formula of WPM + 2 g / L PVP + 0.5 mg / L NAA + 1.0 mg / L 6-BA.

[0035] 3) After the adventitious buds grow, select the leaves produced during the induction process, cut off both ends of the leaves and cut off the veins, and inoculate them into the leaf callus induction with the formula of WPM + 0.5 mg / L NAA + 1.0 mg / L 6-BA and differentiation-inducing medium.

[0036]4) Adventitious buds induced by stem segments and adventitious buds produced by leaf callus differentiation were inoculated on the proliferation medium formulated with WPM + 0.1 mg / L NAA + 1.0 mg / L 6-BA.

[0037] 5) Cut out ...

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Abstract

The invention discloses a tissue culture and rapid propagation method of angel red soft-seed pomegranate, and belongs to the field of plant tissue culture. The method comprises the following technologies: collecting tender stem segments, disinfecting with 75-percent alcohol for 30s,disinfecting with 0.1-percent mercury bichloride for 5 minutes and inoculating the disinfected stem segments on an adventitious bud inducing medium; after the adventitious buds grow out, cutting off two ends of leaves cutting off veins and inoculating to a leaf callus inducing medium; inoculating the adventitious buds produced by stem segment induction and leaf callus differentiation to a proliferation culture medium; transplanting single seedlings growing to 5 to 6 cm to a rooting medium and tempering the seedlings growing to 3 to 4 cm. The tissue culture and rapid propagation method disclosed by the invention has the advantage that rapid proliferation of angel red soft-seed pomegranate tube seedlings can be induced at an induction rate of 92.22 percent to produce a large amount of adventitious buds, and provides an effective way for industrialized seedling production.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a rapid propagation method for pomegranate tissue culture of angel red soft seeds. Background technique [0002] Pomegranate( Punica granatum L.), belonging to Lythraceae (Lythraceae) Pomegranate genus ( Punica L.) Fruit trees have high nutritional value and health care functions. In recent years, pomegranate has become more and more popular in the consumer market, and the pomegranate industry has developed rapidly at home and abroad. Soft-seed pomegranate has soft seed coat, degenerated seed kernel and large grain, which has high value in fresh food consumption. At present, the proportion of soft seed varieties in my country's pomegranate cultivation is relatively small, and the market price is 2-4 times that of ordinary varieties. The market prospect is broad. Angel Red Soft Seed Pomegranate is an excellent variety of soft seed pomegranate imported from the United Stat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 苑兆和火艳徐会丽
Owner 南京榴元生物科技有限公司
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