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44 results about "Liriodendron chinense" patented technology

Liriodendron chinense (commonly known as the Chinese tulip poplar, Chinese tulip tree or Chinese whitewood) is Asia's native species in the genus Liriodendron. This native of central and southern China grows in the provinces of Anhui, Guangxi, Jiangsu, Fujian, Guizhou, Hubei, Hunan, Jiangxi, Shaanxi, Zhejiang, Sichuan and Yunnan, and also locally in northern Vietnam. Protected populations occur in the Tianmushan National Reserve, Huangshan, Wuyi Shan, and Badagongshan Nature Reserve.

Asexual propagation method of michelia compressa

The invention relates to an asexual propagation method of michelia compressa. The asexual propagation method includes the steps that firstly, seedlings of the michelia compressa are manufactured; secondly, pretreatment of a scion of michelia compressa is performed; thirdly, a grafting method is adopted; fourthly, management after grafting is performed. Liriodendron chinense is used as a rootstock, and the cut-grafting method is used for grafting the michelia compressa. After cutting is sequentially soaked into a plant growth regulator and 1%DMSO, the rootstock is inserted, and after the cambium of the scion and the cambium of the rootstock are aligned, an anti-drop film is used for tightly winding and wrapping a port from bottom to top. According to the asexual propagation method of the michelia compressa, a great number of root-included seedlings of the michelia compressa can be obtained, the rate of reproduction is 81-86 times, and after the seedlings are obtained, the seedlings are grafted on the Liriodendron chinense, namely the rootstock, and the survival rate is 95%. After transplantation on a field is achieved for two years, the tree vigor is strong, a tree is resistant to diseases and capable of wintering safely, the problem of vacancy of the market resource of the michelia compressa is effectively solved, and the asexual propagation method of the michelia compressa is wide in application prospect.
Owner:NANJING JINPU LANDSCAPE

Liriodendron chinensis cellulose synthase gene LcCESA1 as well as expression protein and application thereof

The invention discloses a liriodendron chinensis cellulose synthase gene LcCESA1 and as well as expression protein and application thereof. The nucleotide sequence of LcCESA1 is as shown in SEQ ID NO.1; the amino acid sequence of the expression protein is as shown in SEQ ID NO.2. By taking a model plant arabidopsis as a research object, a Chinese liriodendron chinensis cellulose synthase gene LcCESA1 obtained by cloning is mediated and transformed into arabidopsis, and a T3 generation homozygosous gene plant is finally obtained by means of screening and cultivation; by observing microstructures of the wild arabidopsis and transgenetic arabidopsis, and the condition that cell walls of histocytes of vascular bundles of stem sections and the blade histocytes of the transgenetic arabidopsis are different from those of the wild arabidopsis is found, so that the LcCESA1 gene affects the cell walls of histocytes of vascular bundles of stem sections and the blade histocytes of the transgeneticarabidopsis, can promote accumulation of cellulose and has wide application.
Owner:NANJING FORESTRY UNIV

Method for constructing particle gun mediated liriodendron hybrids transformation system

The invention discloses a method for constructing a particle gun mediated liriodendron hybrids transformation system. The method comprises the following steps: 1) taking embryonic callus of liriodendron hybrids, and performing high osmotic treatment; 2) extracting plasmids, preparing gold dust, and preparing micro-particles; 3) performing particle bombardment; 4) transferring the callus to restoreto a normal level to perform dark recovery culture; 5) performing embryoid induction culture, performing light culture after embryoid grows out, and inoculating into a rooting medium to perform screening culture after seedlings grow out; and 6) performing molecular detection, thereby obtaining the liriodendron hybrids transformation system. According to the method for constructing the particle gun mediated liriodendron hybrids transformation system, disclosed by the invention, the feasibility of the particle gun mediated liriodendron hybrids system is explored, and a reliable experimental foundation is provided for transforming liriodendron hybrids by mediating target genes with a particle gun in future, so that a novel technology is created for functional gene transformation of the liriodendron hybrids, and a technical support is provided for researching the effects of various functional genes in the liriodendron hybrids.
Owner:NANJING FORESTRY UNIV

Method for carrying out generation of hybridized liriodendron chinense somatic embryos by utilizing salicylic acid

The invention discloses a method for carrying out generation of hybridized liriodendron chinense somatic embryos by utilizing salicylic acid. The method comprises the following steps: establishing a hybridized liriodendron chinense suspension system, obtaining liquid single cells, transiting and inducing the generation of the hybridized liriodendron chinense somatic embryos; in a process of inducing the generation of the hybridized liriodendron chinense somatic embryos, adding the salicylic acid and ABA (Abscisic Acid) into a culture medium at the same time or independently adding the salicylic acid. According to the method for carrying out the generation of the hybridized liriodendron chinense somatic embryos by utilizing the salicylic acid, disclosed by the invention, a plant growth regulator SA is added in a generation process of the hybridized liriodendron chinense somatic embryos, so that the inductivity of the somatic embryos and the synchronism of development are effectively improved; benefits of breeding hybridized liriodendron chinense somatic cell engineering germchits are improved.
Owner:NANJING FORESTRY UNIV

Identification method for types of descendant tree species of Liriodendron forest

The invention discloses an identification method for the types of descendant tree species of Liriodendron forest and a special kit thereof. The method comprises the following steps: 1) extracting leafDNA (Deoxyribonucleic Acid) of the descendant tree species; 2) performing SSR-PCR (Simple Sequence Repeat-Polymerase Chain Reaction) detection; 3) determining the descendant tree species, wherein thedescendant tree species which can specifically amplify a product of 112bp by electrophoresis are Liriodendron chinense (Hemsl.) Sarg., the descendant tree species which can specifically amplify a product of 124bp by electrophoresis are Liriodendron tulipifera Linn., and the descendant tree species which can specifically amplify products of 112bp and 124bp are hybrid of Liriodendron chinense and Liriodendron tulipifera. The identification method for the types of the descendant tree species of Liriodendron forests disclosed by the invention has the advantages of high repeatability and high stability, can effectively identify the types of descendant tree species of Liriodendron natural forests and artificial forests, particularly the descendant trees of Liriodendron natural forests and artificial forests at a seedling stage, and can identify whether seeds of seed orchards are Liriodendron chinense (Hemsl.). seeds, Liriodendron tulipifera seeds or seeds of hybrid of Liriodendron chinenseand Liriodendron tulipifera. The identification method has the advantages of fastness, high efficiency, accurate result and very high practicality.
Owner:NANJING FORESTRY UNIV

Hybrid liriodendron chinense cutting breeding method

The invention discloses a hybrid liriodendron chinense cutting breeding method. A spraying slotting machine with full illumination and unblocked draining is used, the bottom layer of the slotting machine is laid with cobblestones, and gasp between the cobblestones are filled with sandstones; the middle layer is laid with river sand; the top layer is laid with a cutting matrix, and a bactericide isadded to the cutting matrix before cutting; when a cutting slip is cut, the notch in the lower end is flat, and the notch in the upper end is flat or inclined; the lower end of the cutting clip is immersed with a rooting liquid or dipped with rooting powder; a needed jack is made in the slotting machine in advance, and the lower end of the cutting clip is inserted into the jack; spraying and watering are carried out after cutting immediately, watering is carried out in small amount and frequently, it is ensured that there are water drops on leaves of the cutting clip but there is no accumulated water in the cutting matrix; and the leaves of the cutting clip are sprayed with foliar fertilizers regularly. The rooting rate and survival rate of the hybrid liriodendron chinense can be improvedgreatly, and the method also has the advantages of fast growth, high ornamental value, high stress resistance, high environmental protection performance and wide application prospects.
Owner:福建省漳平五一国有林场

Aureobasidium aureum MR-57 and application thereof

The invention discloses an aureobasidium aureum MR-57 and application thereof, and belongs to the field of functional microorganism screening and application, the strain MR-57 is preserved in Guangdong Microbial Culture Collection Center on November 26, 2021, and the preservation number is GDMCC No: 62084. The bacterial strain MR-57 has relatively strong inhibition and antagonism effects on seven pathogenic bacteria such as column spore destroying bacteria and the like, and has relatively strong antagonism capability on liriodendron alternaria alternata, column spore destroying bacteria and mechanical bacteria echinacea; the sterile fermentation liquor of the strain MR-57 has an obvious inhibition effect on the growth of fusarium equisetum hyphae and can obviously reduce the spore germination rate of fusarium equisetum; a soil mixing method is used for verifying that the strain MR-57 has good colonization capability in soil; a potted plant biocontrol test proves that the strain MR-57 has a certain prevention and growth promoting effect on the divaricate saposhnikovia root rot, and the disease index of the divaricate saposhnikovia root rot can be obviously reduced.
Owner:JILIN AGRICULTURAL UNIV

A method for plant regeneration of pure solid cultured hybrid Liriodendron somatic embryo

The invention discloses a regeneration method adopting pure solid culture for a somatic embryo plant of liriodendron chinense. The method comprises steps as follows: 1) hybrid liriodendron chinense embryonic calli are induced; 2) 3 / 4 MS, VC with concentration of 5mg / L, saccharose with concentration of 30g / L and amino oligochitosan with concentration of 0.01-0.1mg / L are taken as basic media, and the hybrid liriodendron chinense embryonic calli are cultured in dark at 24 DEG C; 3) the material is transferred to a tissue-culture vessel in a light culture room and cultured continuously with the 3 / 4 MS basic medium until the regeneration plant is formed; 4) the regeneration plant is acclimated and transplanted. According to the regeneration method adopting pure solid culture for the somatic embryo plant of liriodendron chinense, the pure solid culture manner is adopted, the somatic embryo induction links including suspension culture and plate culture in the somatic embryo generation process are skipped, a hybrid liriodendron chinense somatic embryo generation system is optimized and enriched, and accordingly, the high-quality somatic embryo regeneration plant is obtained more efficiently and more conveniently; a stable and high-frequency hybrid liriodendron chinense somatic embryo generation system adopting pure solid media and having properties of being easy to operate and good in seedling resistance is established, and reference is provided for establishment of a more efficient genetic transformation receptor system of hybrid liriodendron chinense.
Owner:NANJING FORESTRY UNIV

Asexual propagation method of michelia compressa

ActiveCN103392517BHigh affinityHigh levels of endogenous hormonesHorticulture methodsPlant tissue cultureCambiumBiology
The invention relates to an asexual propagation method of michelia compressa. The asexual propagation method includes the steps that firstly, seedlings of the michelia compressa are manufactured; secondly, pretreatment of a scion of michelia compressa is performed; thirdly, a grafting method is adopted; fourthly, management after grafting is performed. Liriodendron chinense is used as a rootstock, and the cut-grafting method is used for grafting the michelia compressa. After cutting is sequentially soaked into a plant growth regulator and 1%DMSO, the rootstock is inserted, and after the cambium of the scion and the cambium of the rootstock are aligned, an anti-drop film is used for tightly winding and wrapping a port from bottom to top. According to the asexual propagation method of the michelia compressa, a great number of root-included seedlings of the michelia compressa can be obtained, the rate of reproduction is 81-86 times, and after the seedlings are obtained, the seedlings are grafted on the Liriodendron chinense, namely the rootstock, and the survival rate is 95%. After transplantation on a field is achieved for two years, the tree vigor is strong, a tree is resistant to diseases and capable of wintering safely, the problem of vacancy of the market resource of the michelia compressa is effectively solved, and the asexual propagation method of the michelia compressa is wide in application prospect.
Owner:NANJING JINPU LANDSCAPE
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