The invention relates to a culture method of zelkova serrata container big seedlings and belongs to the technical field of plant cultivation. The culture method comprises the steps of garden selection and land preparation, container selection, substrate preparation, seedlings selection, container planting, water and fertilizer management and the like. Zelkova serrata has a slow growing speed, and thus, aiming at the growth characteristics of the zelkova serrata, the culture method adopts a specific culture management, so that the survival rate can be improved, growth of the zelkova serrata is accelerated, the culture period is shortened, the seedlings are enabled to grow into big trees at an early date and natural resources are effectively protected; root control containers used in the culture method have an effect of promoting growth of lateral roots, are unlikely to damage roots in the transplanting process and are beneficial for improving the survival rate of the big seedling transplantation; moreover, in the transplanting process, the crowns of the seedlings do not need to be cut down, full-crown transplantation can be carried out, the transplanting effect is good and the engineering requirements of any construction period can be met.