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Cultivation method of new germplasm of ornamental Dendrobium spp.

A cultivation method and ornamental dendrobium technology are applied in the field of cultivation of new ornamental dendrobium germplasm, which can solve the problems of low germination rate of hybrid seeds, low hybridization affinity, difficult multiplication, etc., and achieve excellent ornamental properties, strong complementarity, stem The effect of beautiful flowers

Active Publication Date: 2014-04-16
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a method for cultivating new germplasm of ornamental Dendrobium, the resulting new germplasm combines the excellent genetic traits of Dendrobium sarcomas and Dendrobium sarcocarpa, and overcomes the hybridization affinity between the aforementioned two kinds of Dendrobium species Low sex, low germination rate of hybrid seeds, difficult multiplication

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The medium formula used is as follows:

[0034] The formula of the seeding medium is 1 / 2MS+white sugar 25g / L+agar powder 4.2g / L+activated carbon 0.5g / L+coconut milk 100ml / L+6-benzylaminopurine 0.1mg / L;

[0035] Proliferation and differentiation medium is 1 / 2MS+peptone 2g / L+sugar 25g / L+agar powder 4.2g / L+activated carbon 1.0g / L+potato 150g / L+banana 50g / L+6-benzylaminopurine 0.1mg / L+naphthalene acetic acid 0.05mg / L L;

[0036] Rooting medium for strong seedlings is 1 / 4N6+ peptone 3g / L+ white sugar 25g / L+ agar powder 4.5g / L+ activated carbon 2.0g / L+ potato 100g / L+ banana 50g / L+ naphthalene acetic acid 0.05mg / L+IBA0.05mg / L;

[0037] (1) Selection of hybrid parents: Select an excellent individual plant of Dendrobium sarcocarpa as the female parent, and an excellent individual plant of Dendrobium sarcocarpa as the male parent;

[0038] (2) Artificial pollination: March 17, 2012 (2 days after the flowers of the female plant to be pollinated open, and 2 days after the flowers...

Embodiment 2

[0047] The medium formula used is as follows:

[0048] The formula of the seeding medium is 1 / 2MS+white sugar 25g / L+agar powder 4.2g / L+activated carbon 0.5g / L+coconut juice 100ml / L+6-benzylaminopurine 0.2mg / L;

[0049] Proliferation and differentiation medium is 1 / 2MS+peptone 2g / L+sugar 25g / L+agar powder 4.2g / L+activated carbon 1.0g / L+potato 150g / L+banana 50g / L+6-benzylaminopurine 0.3mg / L+Naacetic acid 0.1mg / L L;

[0050] Strong seedling rooting medium is 1 / 4N6+ peptone 3g / L+ white sugar 25g / L+ agar powder 4.5g / L+ activated carbon 2.0g / L+ potato 100g / L+ banana 50g / L+ naphthalene acetic acid 0.1mg / L+IBA0.1mg / L;

[0051] (1) Selection of hybrid parents: Select an excellent individual plant of Dendrobium sarcocarpa as the female parent, and an excellent individual plant of Dendrobium sarcocarpa as the male parent;

[0052] (2) Artificial pollination: March 18, 2012 (3 days after the flowers of the female plant to be pollinated open, and 3 days after the flowers of the male plan...

Embodiment 3

[0061] The medium formula used is as follows:

[0062] The formula of the seeding medium is 1 / 2MS+white sugar 25g / L+agar powder 4.2g / L+activated carbon 0.5g / L+coconut milk 100ml / L+6-benzylaminopurine 0.6mg / L;

[0063] Proliferation and differentiation medium is 1 / 2MS+peptone 2g / L+sugar 25g / L+agar powder 4.2g / L+activated carbon 1.0g / L+potato 150g / L+banana 50g / L+6-benzylaminopurine 0.6mg / L+Naacetic acid 0.2mg / L L;

[0064] Strong seedling rooting medium is 1 / 4N6+ peptone 3g / L+ white sugar 25g / L+ agar powder 4.5g / L+ activated carbon 2.0g / L+ potato 100g / L+ banana 50g / L+ naphthalene acetic acid 0.3mg / L+IBA0.3mg / L;

[0065] (1) Selection of hybrid parents: Select an excellent individual plant of Dendrobium sarcocarpa as the female parent, and an excellent individual plant of Dendrobium sarcocarpa as the male parent;

[0066] (2) Artificial pollination: March 26, 2012 (10 days after the flowers of the female plant to be pollinated, and 5 days after the flowers of the male plant for...

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Abstract

The invention discloses a cultivation method of a new germplasm of ornamental Dendrobium spp. The cultivation method comprises the following steps: an inventor chooses a good single plant of Dendrobium pendulum Roxb. as a female parent, and a good single plant of Dendrobium wardianum Warner as a male parent to conduct artificial interspecific hybridization; conducting artificial pollination and management after pollination; picking fruits; sterilizing the surfaces of the fruits; conducting sprouting cultivation, proliferation and differentiation cultivation, strong seedling rooting cultivation, and acclimatization and transplant to obtain a plurality of strongly grown hybrid filial generation grown-up seedlings. The interspecific hybridization filial generation seedlings gather respective good genetic background of the male parent and female parent, and the new germplasm is provided for culturing the ornamental Dendrobium spp. with proprietary intellectual property rights. According to the invention, the problems that the interspecific cross-compatibility of the Dendrobium pendulum Roxb. and the Dendrobium wardianum Warner is relatively low, and the hybrid seedlings are low in germination ratio and difficult to proliferate are solved, so that a foundation is laid for further culturing the new variety of the ornamental Dendrobium spp. with both a stem and a flower are beautiful and good comprehensive characters, and an effective way is provided for breeding the new variety of the ornamental Dendrobium spp.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, in particular to a method for cultivating new germplasm of ornamental dendrobium. Background technique [0002] Dendrobium (Dendrobium spp.) is the general term for Dendrobium plants in the family Orchidaceae. It has important ornamental value. Its flowers are beautiful in shape, bright in color and long in flowering period. Many species are fragrant. They can be used as cut flowers or potted plants. People's favorite, known as one of the "four major ornamental orchids", has an important position in the international orchid market. At present, most of the varieties of Dendrobium cultivated and produced in China are imported from abroad, and there are no new varieties of Dendrobium with independent intellectual property rights in China. [0003] Dendrobium pendulum Roxb., epiphytic, medium plant type, 30-50cm long, pseudobulb internodes are short, nodes swollen in a beautiful bead shape, r...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 龚建英王华新龙定建孙利娜林茂李进华陈宝玲陈尔王珠娜
Owner GUANGXI FORESTRY RES INST
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