Seed production method by interspecific crossing of green abalone and Pacific abalone
A technology of wrinkled plate abalone and hybrid seed production, which is applied in fish farming, application, climate change adaptation, etc., can solve problems such as the loss of offspring of green abalone self-propagation, green abalone and wrinkled plate abalone have not been seen, and have not been successful. Achieving high temperature resistance and remarkable growth rate
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Embodiment 1
[0018] Green abalone and wrinkled pan abalone with good individual vitality and no muscle damage are selected as seed abalones. The average shell length of green abalone is 7.54cm, and the average shell length of wrinkled pan abalone is 8.32cm; the water temperature is controlled at 24°C through circulating water cooling facilities. Females and males were respectively placed in different parental abalone breeding ponds for breeding; after the gonads of the species abalone matured, the individuals with mature gonads were dried in the shade for 70 minutes, and then injected into seawater treated with ultraviolet light at an irradiation intensity of 1000mWh / L, and the seawater temperature was 22°C. Replace the ultraviolet-treated seawater every 60 minutes until the male and female abalones spawn and ejaculate. During the process of artificial induced labor, the male and female breeding abalones of green abalone and wrinkled pan abalone were placed in different containers, and the ...
Embodiment 2
[0020] Similar to Example 1, the difference is that the sperm of the green abalone and the eggs of the wrinkled abalone are mixed, and the concentration of the sperm of the wrinkled abalone is 2.58×10 6 pcs / ml, the result is green abalone ♀ × wrinkled plate abalone The hybrid fertilization rate was 18.32±3.12%. According to the conventional larvae, seedlings and intermediate cultivation methods, the reciprocal abalone seedlings of green abalone and wrinkled abalone were finally obtained. The hybrid grows rapidly, has a high survival rate, is strong in high temperature resistance, has obvious heterosis, and has the potential to develop into a new abalone breeding variety.
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