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Cucumis allostriploid cucumber and used for method of cucumber breeding

A triploid and heterologous technology, applied in the field of chromosome engineering breeding of cucumber, can solve the problem of not cultivating an allotriploid cucumber and the like

Inactive Publication Date: 2003-04-30
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, to date, no allotriploid cucumber of the genus Melon has been bred

Method used

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  • Cucumis allostriploid cucumber and used for method of cucumber breeding
  • Cucumis allostriploid cucumber and used for method of cucumber breeding
  • Cucumis allostriploid cucumber and used for method of cucumber breeding

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 The new species of hybrid amphidiploid between the genus Melon ( figure 2 , 3 ) and ordinary cucumber "Erzaozi" (Southern type cucumber) for germination (new seeds are germinated 30 days in advance), or short-day treatment (light 8hr / dark night 16hr) is adopted for new species to synchronize flowering with ordinary cucumbers. The cucumber pollen was pollinated on the style of the interspecies hybrid new species, and embryos of suitable size were taken for culture after 20 days. Place embryos in MS add 0.2 mg L -1 On the medium of 6-BA, subculture after 40 days, then transplant the regenerated plants into greenhouse or field, after 60 days, you can harvest the seedless fruit ( Figure 4 ). The chromosomes of such plants are 2n=26( Figure 5 ), is an allotriploid cucumber. Resistance analysis showed that it was resistant to weak light and root-knot nematode. Subsequently, through in vitro rapid propagation and preservation, the allotriploid cucumber wi...

Embodiment 2

[0026] Example 2 The allotriploid cucumber cultivated above was used to cross the cucumber female line GY-14, and MS was added with 0.2 mg L -1 Regenerated plants were obtained by culturing on 6-BA medium; heterologous translocation lines were screened out by combining tendril counting chromosomes, C-banding analysis, isoenzyme and RAPD analysis; and then through resistance identification and economic Character evaluation, screened out excellent heterologous translocation line HHZ of mesofruit type (fruit type index 5.0) resistant to root-knot nematodes (root-knot index 4.1), resistant to low light (normal growth under 8000Lx light intensity) 1-9-8 .

[0027] Using female line GY-14 and heterologous translocation line HHZ 1-9-8 Carry out 2 to 4 generations of recurrent breeding, and screen out new varieties of mesocarpus (fruit type index 5.0) resistant to root-knot nematodes (root-knot index 4.1) and resistant to low light (normal growth under 8000Lx light intensity) .

...

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Abstract

A muskmelon-family allotripolid cucumber without seed is bred by interspecific hybridization between the interspeiescrossed didiploid muskmelon and ordinary cucumber. An alloheterotopic line of cucumber with weak light tolerance and the resistance to root nematoda is obtained by the backcross between said allotriploid cucumber and ordinary cucumber. A new variety of cucumber is obtained by the hybridization between said alloheterotopic line and the female-line cucumber.

Description

1. Technical field [0001] The invention discloses melon allotriploid cucumbers and a method for breeding cucumbers, belonging to the field of biotechnology breeding, and is specially used for chromosome engineering breeding of cucumbers. 2. Technical background [0002] In the patent "Methods for Cucumber Breeding of Wild Melon Species" (Application No. 00 1 12271.1---9c) applied by our research group, we overcome the gap between wild species of Melon and cultivated species for the first time by rescuing immature embryos. interspecific hybrid barrier; using somatic variation, the double haploid interspecific hybrid was successfully doubled in chromosome, and then after fertility recovery screening, the world's first interspecific hybrid double diploid species of Melon was bred C. hytivus. This new amphidiploid species carries the complete set of chromosomes of the wild species C.hystrix, but as an allotetraploid, the new amphidiploid species has low fertility, which is not ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 陈劲枫罗向东钱春桃庄飞云任刚
Owner NANJING AGRICULTURAL UNIVERSITY
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