Method for acquiring non-heading Chinese cabbage clubroot resistance germplasm resources

Abstract

The invention discloses a method for obtaining germplasm resources of non-heading Chinese cabbage resistant to clubroot, comprising: selecting introduced Chinese cabbage resistant to clubroot and susceptible non-heading Chinese cabbage with excellent agronomic properties; Interspecific hybridization was used to obtain F1 seeds of the first generation of hybridization; the screened F1 generation was continuously backcrossed for 4 generations, and a new non-heading Chinese cabbage resistant germplasm Q15‑1 with stable phenotype and resistance to clubroot was initially obtained. The present invention adopts the method of interspecific hybridization, and carries out artificial inoculation identification on each backcross offspring, screens the resistant single plant of clubroot disease, and finally obtains new germplasm. The present invention utilizes Brassicaceae Chinese cabbage The hybrid vigor between the two varieties of non-heading Chinese cabbage and its variant non-heading Chinese cabbage, the resistance gene of clubroot Chinese cabbage is transferred to non-heading Chinese cabbage through hybridization and backcrossing at the bud stage, which is simple and easy. Biological risks caused by other methods such as genetic modification and genetic engineering technology are excluded.

Description

technical field [0001] The invention belongs to the field of innovative research and development of germplasm of agricultural crops, and in particular relates to a method for obtaining germplasm resources of non-heading Chinese cabbage resistant to clubroot. Background technique [0002] Clubroot of cruciferous crops is a root disease of cruciferous crops caused by the infestation of plasmodiophora brassicae Woron, an obligate parasite that specifically infects cruciferous crops. The pathogen invades the root hairs, cortex, and cambium of plants, produces pathogenic secretions containing plant growth hormone, stimulates the abnormal division and enlargement of parenchyma cells, and expands to form nodular roots at the hyperplasia. On the one hand, the appearance of swollen roots reduces the ability of the root system to absorb and supply water and nutrients, causing the aboveground part to lose water, wither and die; on the other hand, the plant root system loses the protect...

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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for obtaining the germplasm resources of non-heading Chinese cabbage resistant to clubroot, aiming to solve the lack of methods and lack of methods for creating germplasm resources of non-heading Chinese cabbage resistant to clubroot in the prior art. Problems with formed clubroot resistant green vegetable cultivars

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