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Selection and reproduction method for new variety of flue-cured tobacco sterile line

A new and variety technology, applied in the field of tobacco genetics and breeding, can solve problems such as the decline of variety resistance, the loss of flue-cured tobacco production, and the damage of diseases and insect pests.

Active Publication Date: 2016-01-06
ZUNYI TOBACCO OF GUIZHOU TOBACCO CORP
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Problems solved by technology

In recent years, due to the fact that there are very few stereotyped varieties selected in my country's flue-cured tobacco breeding, the main varieties planted in the national tobacco area are single, the variety resistance is reduced, and the damage of diseases and insect pests is widespread, which has caused serious losses to my country's flue-cured tobacco production.

Method used

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  • Selection and reproduction method for new variety of flue-cured tobacco sterile line

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Embodiment Construction

[0031] Embodiments of the invention:

[0032] The flue-cured tobacco varieties MSG28 and TZ-1 used in the present invention are all purchased from the National Germplasm Resource Bank of Qingzhou Tobacco Research Institute of China National Tobacco Corporation.

[0033] The method for breeding new varieties of flue-cured tobacco sterile lines comprises the following steps:

[0034] (1) Hybridization: The male sterile hybrid combination F was obtained by crossing the male sterile line MSG28 as the female parent and TZ-1 as the male parent. 1 seed, F 1 Seeds are mixed and harvested, and the seed number is saved;

[0035] (2) Continuous backcrossing: in the next year, plant the male sterile hybrid combination F 1 and TZ-1, a male sterile cross combination F 1 as the female parent, TZ-1 as the reincarnation parent, backcross to obtain BC 1 f 1 seed, bc 1 f 1 Seeds are mixed and harvested; in the following years, combined with Hainan Jiadai, TZ-1 is used as the recurrent ...

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Abstract

The invention discloses a selection and reproduction method for a new variety of a flue-cured tobacco sterile line. The method comprises the following steps: hybridizing flue-cured tobacco cytoplasm male sterile line MSG28 serving as a cytoplasm sterile gene source which is a female parent and the variety TZ-1 serving as a recurrent parent, then continuously backcrossing for 6 generations, and culturing a novel cytoplasm male sterile line MSTZ-1; outbreeding the sterile lineM MSTZ-1 serving as the female parent and a maintainer line TZ-1 serving as a male parent to save the sterile line MSTZ-1. The flue-cured tobacco sterile line MSTZ-1 selected by the selection and reproduction method has the advantages of stable sterility, high integrated resistance, high tobacco leaf appearance internal quality, coordinative chemical component and the like, and a new good sterile line variety is provided for the research on the advantage utilization of flue-cured tobacco hybrids.

Description

technical field [0001] The invention belongs to the technical field of tobacco genetics and breeding, and in particular relates to a method for selection and propagation of flue-cured tobacco cytoplasmic male sterile lines. Background technique [0002] Flue-cured tobacco has strong heterosis performance in yield, disease resistance, growth period, growth vigor, etc., but because there is almost no obvious heterosis in tobacco leaf quality when crossing between flue-cured tobacco varieties, the work of utilizing flue-cured tobacco heterosis was once used by flue-cured tobacco breeding. Workers give up. In recent years, due to the fact that there are very few stereotyped varieties selected for breakthrough in flue-cured tobacco breeding in my country, the main varieties planted in the tobacco-growing areas of the country are single, the variety resistance is reduced, and pests and diseases are harmful to a large area, causing serious losses to flue-cured tobacco production in...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
Inventor 王军陈晓明温明霞冯娅
Owner ZUNYI TOBACCO OF GUIZHOU TOBACCO CORP
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