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A method for obtaining hybrids between water lily varieties

A technology for hybrids and varieties, applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc., can solve problems such as obstacles, lack of seeds for hybridization, affecting the color of water lilies, and recombination of genes with excellent leaf color, etc. Effects of genetic basis

Active Publication Date: 2016-06-08
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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Problems solved by technology

The hybrid breeding of foreign water lilies has been carried out for 150 years, and a large number of new varieties have been selected and bred. However, the breeding of new varieties of water lilies in China has just started at the end of the last century. There are barriers after fertilization in intercrossing, so that seeds cannot be obtained by hybridization, which seriously affects the recombination of excellent genes such as water lily flower color and leaf color. Therefore, solving this problem is of great significance for the selection of new water lily varieties.

Method used

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Embodiment Construction

[0015] The method provided by the invention to obtain the hybrid between water lily varieties, its embodiment is as follows:

[0016] 1.1 Material selection

[0017] 'Fenzhuang' (Nymphaea 'FenZhuang') and 'White Fairy' (Nymphaea 'Gonnere') (both known varieties, see references Huang Guozhen, Deng Huiqin, Li Zuxiu, Li Gang. Water Lily. China Forestry Press, Beijing, 2001) , 'Fenzhuang' has pink flowers and a star shape, while 'Baixianzi' has white flowers and a spherical shape. If the flower form of 'Baixianzi' is transferred to 'Fenzhuang', beautiful pink flowers with multiple petals will be bred. In fact, after years of hybridization practice, 'Fenzhuang' and 'Baixianzi' can not be crossed. Hybrids, the main reason is the presence of post-fertilization disorders.

[0018] 1.2 Obtaining seedlings of hybrids of 'Fenzhuang' and 'Baixianzi'

[0019] 'Fenzhuang' was used as the female parent, and 'Baixianzi' was used as the male parent for artificial hybridization. The parents ...

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Abstract

The invention relates to a method for obtaining hybrid seeds of water lily varieties and belongs to the technical field of plant breeding. The method comprises the main technical measures of after intervarietal hybridization is performed, performing asepsis treatment on pollinated 14-16-days ovaries after pollination, taking out embryos from the ovaries, and cultivating the embryos serving as explants in a culture medium with 1 / 2MS added with 1.5mg / L of 2,4-D of and 2.0mg / L of 6-BA, performing subculture and expanding propagation to obtain seedlings, and transferring the seedlings into a culture medium with MS added with 0.2mg / L of NAA for rooting, wherein the culture temperature is 20-25 DEG C, illumination time is 10-12 hours every day, and the illuminance is 1,600-2,000lx. According to the method, the key technical problem of obstacle caused by hybridization and fertilization of the water lily varieties can be solved, and seedlings of the hybrid seeds of the water lily varieties can be obtained.

Description

technical field [0001] The invention relates to a method for obtaining hybrids among water lily varieties, and belongs to the technical field of plant breeding. Background technique [0002] Water lily is a general term for plants of the genus Nymphaea in the family Nymphaeaceae. There are 40-50 species of water lilies in the world, and 5 species in my country. Water lilies are deeply loved by people for their rich colors, holy implication, and strong adsorption capacity of stems and leaves to nutrient and harmful substances in water, and are widely used in garden waterscapes. [0003] At present, hybrid breeding among varieties is still one of the main methods for the selection and breeding of new varieties of flowers. The hybrid breeding of water lilies in foreign countries has been carried out for 150 years, and a large number of new varieties have been selected and bred. However, the breeding of new varieties of water lilies in China has just started at the end of the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 孙春青潘跃平马志虎戴忠良毛忠良吴国平秦文斌姚悦梅张振超潘永飞孙国胜王建华陈智超
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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