Nymphaea breeding method

A water lily and female parent technology, applied in the field of water lily breeding, can solve the problems of interspecific barriers between cold-resistant water lilies and tropical water lilies, difficult to achieve breeding goals, and reduced breeding efficiency, so as to promote the growth of pollen tubes, overcome hybridization obstacles, and scientific methods reasonable effect

Pending Publication Date: 2020-07-10
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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Problems solved by technology

However, in the breeding process of water lilies, breeders often encounter the situation that cold-resistant water lilies and tropical water lilies are not fertile, which greatly reduces the breeding efficiency of water lilies, making it difficult to achieve many breeding goals, and the breeding process takes a long time. Reduced breeding efficiency
At present, there is no report on solving the problem of poor seed setting rate between cold-resistant water lilies and tropical water lilies

Method used

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  • Nymphaea breeding method

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Effect test

Embodiment 1

[0023] 1. Material selection

[0024] 'Peter' (Nymphaea'Peter Slocum') and Xiaohua (Nymphaea micrantha) (both known species, see references Huang Guozhen, Deng Huiqin, Li Zuxiu, Li Gang. Water Lily. China Forestry Press, Beijing, 2009), 'Peter' flower color It is a light pink flower with a cup-shaped flower shape, while the small flower color is light blue-purple with a star-shaped flower shape. If the flower color of the small flower is transferred to 'Peter', a light blue-purple flower with multiple petals and beautiful color will be bred. In fact, after many years of hybridization practice, the hybrid between 'Peter' and the small flower cannot be obtained. The main reason is that there is Prefertilization disorders. The self-fertilization rates of 'Peter' and Xiaohua were 75% and 66%, respectively.

[0025] 2. Obtaining hybrids of 'Peter' and Xiaohua, specifically including the following steps:

[0026] Step 1. Bagging and isolating the buds of 'Peter' and the florets o...

Embodiment 2

[0032] In order to study the influence of flavonol concentration on the hybridization effect of 'Peter' and florets, 4 groups of experiments were set up in this embodiment, and the flavonol concentration is shown in Table 1; the hybridization method adopts the hybridization method of the present invention, and the specific steps are the same as in Example 1. Hybridization The effect is shown in Table 1. The hybrid seeds obtained in each group of experiments were sown, and plants with different traits were obtained in the second year. The results can be seen from Table 1. The addition of different concentrations of flavonols has no significant effect on the seed setting rate of 'Peter' × floret hybrids, but can significantly increase the pollen of florets. The number of germinations on the stigma of 'Peter', resulting in more true hybrids.

[0033] Table 1 Effects of different flavonol concentrations on the hybridization effect

[0034]

[0035] Note: Duncan's multiple ramg...

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Abstract

The invention discloses a nymphaea breeding method, and belongs to the technical field of flower breeding. The nymphaea breeding method comprises the following steps: collecting fresh male parent pollen, female parent pollen and female parent stigmatic fluid, adding flavonol into the female parent stigmatic fluid, and conducting mixing to obtain a mixture used as a pollinating solution; after thefresh female parent pollen, the fresh male parent pollen and the pollinating solution are mixed, conducting pollinating to obtain hybrid seeds; and sowing the hybrid seeds, keeping plants with characters inconsistent with those of a female parent, and conducting directive breeding. By means of the nymphaea breeding method, the problems, especially the problems of barrenness due to interspecific hybridization of cold-resistant nymphaea and tropical nymphaea, of barrenness due to interspecific hybridization of nymphaea are solved, the female parent pollen and the male parent pollen are mixed, and the flavonol is added into the pollinating solution so that germination of the male parent pollen on stigmas of the female parent can be promoted, and a novel method is provided for breeding of novel nymphaea varieties.

Description

technical field [0001] The invention belongs to the technical field of water lily breeding, and in particular relates to a water lily breeding method. Background technique [0002] Water lily is a general term for plants of the genus Nymphaea in the family Nymphaeaceae. In recent years, with the development of leisure agriculture and rural tourism, water surface greening urgently needs a large number of new water lily varieties with rich colors and long flowering periods. However, in the breeding process of water lilies, breeders often encounter the situation that cold-resistant water lilies and tropical water lilies are not fertile, which greatly reduces the breeding efficiency of water lilies, making it difficult to achieve many breeding goals, and the breeding process takes a long time. Breeding efficiency decreased. At present, there is no report on solving the problem of poor seed setting rate of interspecific barriers between cold-resistant water lilies and tropical ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01N43/16A01P21/00
CPCA01H1/02A01N43/16
Inventor 孙春青戴忠良姚悦梅徐迎春山溪
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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