Nymphaea breeding method
A water lily and female parent technology, applied in the field of water lily breeding, can solve the problems of interspecific barriers between cold-resistant water lilies and tropical water lilies, difficult to achieve breeding goals, and reduced breeding efficiency, so as to promote the growth of pollen tubes, overcome hybridization obstacles, and scientific methods reasonable effect
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Embodiment 1
[0023] 1. Material selection
[0024] 'Peter' (Nymphaea'Peter Slocum') and Xiaohua (Nymphaea micrantha) (both known species, see references Huang Guozhen, Deng Huiqin, Li Zuxiu, Li Gang. Water Lily. China Forestry Press, Beijing, 2009), 'Peter' flower color It is a light pink flower with a cup-shaped flower shape, while the small flower color is light blue-purple with a star-shaped flower shape. If the flower color of the small flower is transferred to 'Peter', a light blue-purple flower with multiple petals and beautiful color will be bred. In fact, after many years of hybridization practice, the hybrid between 'Peter' and the small flower cannot be obtained. The main reason is that there is Prefertilization disorders. The self-fertilization rates of 'Peter' and Xiaohua were 75% and 66%, respectively.
[0025] 2. Obtaining hybrids of 'Peter' and Xiaohua, specifically including the following steps:
[0026] Step 1. Bagging and isolating the buds of 'Peter' and the florets o...
Embodiment 2
[0032] In order to study the influence of flavonol concentration on the hybridization effect of 'Peter' and florets, 4 groups of experiments were set up in this embodiment, and the flavonol concentration is shown in Table 1; the hybridization method adopts the hybridization method of the present invention, and the specific steps are the same as in Example 1. Hybridization The effect is shown in Table 1. The hybrid seeds obtained in each group of experiments were sown, and plants with different traits were obtained in the second year. The results can be seen from Table 1. The addition of different concentrations of flavonols has no significant effect on the seed setting rate of 'Peter' × floret hybrids, but can significantly increase the pollen of florets. The number of germinations on the stigma of 'Peter', resulting in more true hybrids.
[0033] Table 1 Effects of different flavonol concentrations on the hybridization effect
[0034]
[0035] Note: Duncan's multiple ramg...
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