In-vitro oriented screening method of peanut high-oil body
A technology for directional screening and peanuts, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of difficult cultivation of high-yield and high-oil peanut varieties, lack of high-oil germplasm resources of peanuts, etc., and save domestication and cultivation time , Reduce the time of hardening seedlings, save resources and cost
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[0024] A method for in vitro directional screening of peanut high oil bodies according to the present invention comprises the following steps:
[0025] 1. Preparation of somatic embryo induction medium
[0026] (1) Prepare a somatic embryo induction medium, select MS medium as the basic medium, and add 2,4-D to the MS medium to form a somatic embryo induction medium. The amount of 2,4-D added is 2-12 mg / L. The somatic embryo induction medium in this example is MS+5mg / L2,4-D+30g / L sucrose+8g / L agar; the pH of the somatic embryo induction medium was adjusted to 5.8.
[0027] 2. Preparation of medium for somatic embryo germination into seedlings and high oil body directional selection
[0028] (1) Preparation of somatic embryo germination and high-oil screening medium: MS medium was selected as the basic medium, and hydroxyproline and 6-benzylaminopurine (BAP) were added to this MS medium to form somatic embryo germination and high-oil screening medium. Oriented screening medi...
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