Breeding and propagation method of sterile line novel variety of flue-cured tobacco

A new and variety-based technology, applied in the field of tobacco genetics and breeding, can solve the problems of decreased variety resistance, single main variety in tobacco-growing areas, and damage from diseases and insect pests

Active Publication Date: 2015-12-16
ZUNYI TOBACCO OF GUIZHOU TOBACCO CORP
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Problems solved by technology

In recent years, due to the fact that there are very few stereotyped varieties selected in my country's flue-cured tobacco breeding, the main varieties planted in the national tobacco...

Method used

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  • Breeding and propagation method of sterile line novel variety of flue-cured tobacco

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Experimental program
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Embodiment Construction

[0031] Embodiments of the invention:

[0032] The flue-cured tobacco varieties MSG28 and CZ-1 used in the present invention are all purchased from the National Germplasm Resource Bank of Qingzhou Tobacco Research Institute of China National Tobacco Corporation.

[0033] The method for breeding new varieties of flue-cured tobacco sterile lines comprises the following steps:

[0034] (1) Hybridization: The male sterile hybrid combination F was obtained by crossing the male sterile line MSG28 as the female parent and CZ-1 as the male parent. 1 seed, F 1 Seeds are mixed and harvested, and the seed number is saved;

[0035] (2) Continuous backcrossing: in the next year, plant the male sterile hybrid combination F 1 and CZ-1, a male sterile cross combination F 1 As the female parent, with CZ-1 as the recurrent parent, backcross to obtain BC 1 f 1 seed, bc 1 f 1 Seeds are mixed and harvested; in subsequent years, combined with Hainan Jiadai, CZ-1 is used as the recurrent pa...

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Abstract

The invention discloses a breeding and propagation method of a sterile line novel variety of flue-cured tobacco. The method comprises the steps that a cytoplasmic male sterile line MSG28 of the flue-cured tobacco serves as a source of cytoplasm sterile genes, MSG28 serves as a female parent, CZ-1 serves as a recurrent parent, after hybridization is conducted, backcross is continuously conducted for six generations and a novel cytoplasmic male sterile line MSCZ-1 is obtained through cultivation; the sterile line MSCZ-1 serves as a female parent, a maintainer line CZ-1 serves as a male parent, outbreeding is conducted, and the sterile line MSCZ-1 is preserved. The flue-cured tobacco sterile line MSCZ-1 bred through the method has the advantages of being stable in sterility, excellent in agronomic trait, high in yield and output value, good in easy-curing property and curing resistance, good in appearance and internal quality of tobacco leaves, coordinated in chemical composition and the like, and novel excellent sterile line germplasm is provided for heterosis utilization research work of the flue-cured tobacco.

Description

technical field [0001] The invention belongs to the technical field of tobacco genetics and breeding, and in particular relates to a method for selection and propagation of flue-cured tobacco cytoplasmic male sterile lines. Background technique [0002] Flue-cured tobacco has strong heterosis performance in yield, disease resistance, growth period, growth vigor, etc., but because there is almost no obvious heterosis in tobacco leaf quality when crossing between flue-cured tobacco varieties, the work of utilizing flue-cured tobacco heterosis was once used by flue-cured tobacco breeding. Workers give up. In recent years, due to the fact that there are very few stereotyped varieties selected for breakthrough in flue-cured tobacco breeding in my country, the main varieties planted in the tobacco-growing areas of the country are single, the variety resistance is reduced, and pests and diseases are harmful to a large area, causing serious losses to flue-cured tobacco production in...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 王军夏志林温明霞饶兴义沈子霞
Owner ZUNYI TOBACCO OF GUIZHOU TOBACCO CORP
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