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QTL related to soybean hundred-grain weight, molecular marker, amplification primer and application

A technology of amplification primers and molecular markers, which is applied in the direction of recombinant DNA technology, biochemical equipment and methods, measurement/testing of microorganisms, etc., can solve the problem of single genetic diversity and achieve the effect of broadening the genetic basis

Active Publication Date: 2022-03-01
JILIN ACAD OF AGRI SCI
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Problems solved by technology

Moreover, most of the parents of the population are cultivated species, and the long-term domestication selection by humans has resulted in relatively single genetic diversity.

Method used

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  • QTL related to soybean hundred-grain weight, molecular marker, amplification primer and application
  • QTL related to soybean hundred-grain weight, molecular marker, amplification primer and application
  • QTL related to soybean hundred-grain weight, molecular marker, amplification primer and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Acquisition of QTLs and molecular markers related to soybean hundred-seed weight

[0027] 1. Construction of mapping groups

[0028] In 2006, Suinong 14 (SN14) was used as the recurrent parent, and wild soybean ZYD00006 was used as the donor parent, through continuous backcrossing and selfing, a genome-wide introduction line population was constructed. see specific generation figure 1 .

[0029] 2. Population genotype detection

[0030] The population was resequenced to detect the genotype (the process is as follows figure 2 shown), the steps include:

[0031] (1) Using the CTAB method to extract the DNA of the parent and offspring strains. After the sample is qualified, the DNA is randomly interrupted by ultrasonic crushing, and the DNA fragment is repaired at the end, A is added to the 3' end, a sequencing adapter is added, purification, and PCR amplification are performed to complete the construction of the sequencing library. After passing the qual...

Embodiment 2

[0048] Example 2: Application of QTL and molecular marker SSR-09-089 related to soybean hundred-grain weight According to the gene sequence of SSR-09-089, amplification primers were designed, and the amplification primers were:

[0049] SSR-09-089F: 5'-TCATTTTTGACAATGCTTGAAA-3', as shown in SEQ ID NO.2;

[0050] SSR-09-089R: 5'-TTGGAACAGACGAGCAAAAA-3', as shown in SEQ ID NO.3.

[0051] The extreme 100-seed weight phenotype material was selected, and the genomic DNA of soybean leaves was extracted by CTAB method, and amplified with the above-mentioned amplification primers. The PCR reaction system and procedures are shown in Table 4 and Table 5. The amplified products were detected by polyacrylamide gel electrophoresis. The result is as Figure 7 As shown, the electrophoresis results of each strain were compared with the parents. The band pattern identical to the parent SN14 was recorded as 1, the same as the parent ZYD00006 was recorded as 2, and the heterozygous one was reco...

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Abstract

The invention relates to the field of soybean molecular breeding, in particular to a QTL related to soybean hundred-grain weight, a molecular marker, an amplification primer and application. Cultivated soybeans and wild beans are used as parents to construct a genetic population, the genetic population is positioned to one QTL related to the soybean hundred-grain weight, the QTL is located in the 1641719bp-1795446bp interval of the No.7 chromosome of the soybeans, the marker interval is only 0.15 Mb, and a molecular marker SSR-09-089 related to the hundred-grain weight is identified and can be used for molecular marker-assisted selective breeding and mining of related functional genes; meanwhile, wild resources can be utilized, and the genetic basis of soybean cultivation is widened.

Description

technical field [0001] The invention relates to the field of soybean molecular breeding, in particular to a QTL related to soybean hundred-grain weight, molecular markers, amplification primers and applications. Background technique [0002] 100-seed weight is an important factor of soybean yield, and is closely related to the appearance quality of soybean. Variety improvement for 100-seed weight traits is of great significance for improving soybean yield and quality. However, 100-seed weight is a complex quantitative trait with high heritability and controlled by multiple genes. The traditional breeding cycle is long and difficult, requiring a lot of manpower and material resources. With the development of molecular genetics, molecular marker-assisted selection breeding provides a new way for people to speed up the breeding process. Marker-assisted selection breeding refers to the indirect selection of target traits through molecular markers linked to functional genes dur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/6895C12Q1/686C12Q2600/13C12Q2565/125
Inventor 蒋洪蔚谢建国陈庆山刘春燕郑海洋魏茹侯立龙曹富斌
Owner JILIN ACAD OF AGRI SCI
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