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56 results about "Growing sheep" patented technology
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Method for breeding meat and wool dual-purpose new variety of Gansu alpine merino
The invention discloses a method for breeding a meat and wool dual-purpose new variety of Gansu alpine merino, which comprises: crossing a South African mutton merino serving as a male parent and a Gansu alpine merino serving as a female parent, and selecting first-generation hybrid female merinos; crossing the select first-generation hybrid female merinos with South African mutton merinos, and selecting second-generation hybrid male and female merinos; crossing the selected second-generation hybrid female merinos with South African mutton merinos and selecting third-generation hybrid female merinos; mating the third-generation hybrid female merinos with the selected second-generation hybrid male merinos to breed the zero-generation merinos of the meat and wool dual-purpose new variety of Gansu alpine merino; selecting high-quality male merinos and female merinos; and performing 3 to 4 generations of crossing by a group secondary selection method and obtaining the meat and wool dual-purpose new variety of Gansu alpine merino. The merinos bred by the breeding method have the advantages of tall and big body, high lambing percentage, quick growth, high wool quality, high meat quality and the like, and are suitable for drylot feeding and pen feeding for fattening. The method is in accordance with the development trend of sheep breeding industry of China.
Owner:GANSU AGRI UNIV
Breeding ewe complete pellet feed and preparation method thereof
InactiveCN104256186AFine grainIncrease productionFood processingAnimal feeding stuffBiotechnologyAgricultural science
The invention discloses cultivation feed, particularly relates to breeding ewe complete pellet feed and belongs to the technical field of feed cultivation. The breeding ewe complete pellet feed has the benefits that the nutrition collocation is reasonable, a positive combined effect between roughage (peanut shells, soybean hulls, brewer's grain, apple pomace and the like) and concentrated feed is utilized, the dietary nutrition is balanced and completely meets the production and breeding demands of breeding ewes, the digestion and the utilization of coarse-fibered raw materials are greatly improved, the normal physiological functions of rumens of the breeding ewes are ensured, the feeding process of farmers are greatly reduced at the same time, the production efficiency is improved, the market demands and the benefits are huge, and meanwhile, the multiple coarse-fibered raw materials are utilized by adopting a special processing mode and other measures, so that the social value is higher.
Owner:SICHUAN TQLS IND
Sheep frozen semen dilution and process used for preparing sheep straw frozen semens
InactiveCN104705290AIncrease temperatureProne to exhaustionDead animal preservationGrowing sheepOvis
The invention relates to the technical field of sheep frozen semen production, in particular to sheep frozen semen dilution and a process used for preparing sheep straw frozen semens. The sheep frozen semen dilution is prepared by the following steps: 1, preparing basic liquid; 2, preparing dilution A; 3, preparing dilution B, wherein the dilution A obtained in the step 2 and the dilution B obtained in the step 3 are to-be-obtained sheep frozen semen dilution. The sheep frozen semen dilution is used in the process for preparing the sheep straw frozen semens. The process used for preparing the sheep straw frozen semens mainly comprises the following steps: 1, diluting and balancing a sheep frozen semen fluid; 2, preparing the straw frozen semens of the sheep frozen semen fluid; 3, performing freezing and warehousing. The sheep frozen semen dilution provided by the invention greatly improves the bioactivity of sheep semens, improves production quality, and is economic and practical; the process used for preparing the sheep straw frozen semens is quick and convenient to operate, economic and practical, saves time, and facilitates improvement of the semen quality of breeding sheep and increment of annual semen volume of the breeding sheep.
Owner:XINJIANG TIANSHAN ANIMAL HUSBANDRY BIO ENG
Method for cultivating meat and fine hair goat
InactiveCN101120670AAdaptableStrong disease resistanceAnimal husbandryBoer goat breedFirst generation
The present invention relates to the technical field of animal breeding, in particular to a breeding method of the meat-cashmere goat, which resolves the problems of the prior meat goat and the prior Cashmere goat that the meat output and the cashmere output are not balanced. The present invention firstly selects the breeding goat of the Inner Mongolian cashmere goat to be mated with the local excellent-quality original goat to get the first generation of the hybrid variety; and then selects the KELAN breeding cashmere goat, the Asian gazelle, the Boer goat, the cashmere goat in Gaizhou of Liaoning and the new generation of Liaoning cashmere as the male parent to mate with the offspring of the excellent hybrid goat so as to finally get a new variety of the meat-cashmere goat. The present invention adopts the diversity hybrid way to form a new goat strain both for the meat and the cashmere after breeding in more than twenty years, which integrates the advantages of the meat goat and the cashmere goat and has the advantages that the goat is heavy when born, the weight is heavy and the growth is fast; the meat yield and the cashmere yield are high; the sex maturation is early and the production rate is high; after breeding in large scale, the present invention has important significance for improving the life of the people and developing the cultivation industry, which has a favorable economic benefit and the social benefit.
Owner:姜俊青
Cultivation method for Yunan semi-fine wool sheep
InactiveCN103270999AQuality indicators unchangedScientific and reasonable farming methodsAnimal husbandryData managementAnimal husbandry
The invention discloses a cultivation method for Yunan semi-fine wool sheep, and relates to the technical field of animal husbandry cultivation. The cultivation method for the Yunan semi-fine wool sheep comprises the steps of (1) improving a variety identification standard and a variety grading standard of the Yunan semi-fine wool sheep, (2) building a standard breeding record file and a breeding data management system of the Yunan semi-fine wool sheep, starting to apply the standard breeding record file and the breeding data management system of the Yunan semi-fine wool sheep in a breeding farm of the Yunan semi-fine wool sheep, standardizing breeding data and breeding sheep management, (3) formulating an evaluation scheme of main economic character inheritance of the Yunan semi-fine wool sheep, (4) carrying out breeding sheep production performance measurement and wool quality analysis of the Yunan semi-fine wool sheep, (5) applying a new breeding technology in improved variety production and variety improvement, (6) formulating the technical scheme of lamb cultivation and fat lamb production, and (7) formulating regulations of cultivation management and regulations of epidemic prevention of the Yunan semi-fine wool sheep. The cultivation method for the Yunan semi-fine wool sheep is scientific and reasonable, adjustment is carried out on an index of the weight of first-grade sheep and an index of wool yield of the first-grade sheep, and the index of wool quality is not changed.
Owner:SHAOTONG JINNIU GREEN IND
Sheep breeding method
InactiveCN104351122AGuaranteed uniform conceptionStrengthen all aspects of the bodyAnimal husbandryEstrus synchronizationFodder
The invention discloses a sheep breeding method. The method comprises the steps of selection of a breeding sheep, preconception management, estrus synchronization treatment, artificial impregnation, conception management, preparation before delivery and delivery. In the breeding process of sheep, preconception culture is taken into consideration, concentrated feeds serve as main feeds, rough feeds serve as auxiliary feeds, various conditions of the body of the breeding sheep are enhanced, and the physical quality of a sheep baby after birth is guaranteed; meanwhile, the delivery is expedited by medicines, the conception consistency of the sheep is guaranteed, a pregnant sheep is convenient to manage, and the management difficulty and the management cost are reduced.
Owner:颍上县润海畜禽养殖股份有限公司
A Molecular Marking Method for Sheep Wool Curl
InactiveCN102286628AReduce breeding costsSpeed up the breeding processMicrobiological testing/measurementEnzyme digestionLeast squares
The invention provides a molecular marking method for sheep wool curl, which relates to a molecular marking method, which comprises the following steps of: 1, extracting the sheep genome deoxyribonucleic acid (DNA) for carrying out polymerase chain reaction (PCR) amplification and then carrying out enzyme digestion to obtain enzyme digestion products; 2, carrying out electrophoresis separation onthe enzyme digestion products and carrying out genotype judgment according to the electrophoresis separation results; 3, carrying out association analysis and evaluating least square mean value of the property to obtain a result that the wool curl of individuals with the TT genotype type and TC genotype in the three genotypes is obviously higher than the wool curl of individuals with the CC genotype; and 4, dividing experiment groups into three types according to the result in the third step to complete the operation of the molecular marking method. The molecular marking method can be used for wool curl and fineness molecular marking auxiliary culture, the early period selection of the breeding sheep can be realized, and the sheep can be selected after the birth, so the breeding cost can be reduced, the breeding process of high-quality fine wool sheep is accelerated, the operation is simple, the accuracy is high, the efficiency is high, and the automatic detection can be realized.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Breeding method for three-breed crossed sheep
The invention provides a technique for breeding high-quality sheep through a three-breed crossing method, and belongs to the field of livestock breeding. By taking small tailed han sheep (named 'han' for short) with the higher multiple pregnancy rate as a female parent, fully utilizing poll dorset (named 'tao' for short) and bald suffolk (named 'sa' for short) which are good in mutton quality and rapid to grow as male parents and adopting the important techniques such as first generation target matching crossing, second generation interlacing crossing, third generation and fourth generation backcrossing, high generation mass mating, type stable selecting and series variety selecting of multi-type three-breed crossing, three-breed crossed breeding sheep of multiple types of the 'han, tao, sa' sheep are bred, the three-breed crossed breeding sheep is high in multiple pregnancy rate, good in mutton quality, rapid to grow and wide in application and integrates the advantages of the three types of sheep, and multiple types of breeding sheep such as 'tao-similar' sheep, 'sa-similar' sheep and 'han-similar' sheep which are similar to the different types and have the mutton-wool dual purposes are generated.
Owner:张家口市禾惠农业科技有限公司
Luoping red goat purification and rejuvenation method
The invention discloses a Luoping red goat purification and rejuvenation method; the method comprises the following steps of: goat selection, core-field breeding goat matching and grouping measures: goat selection: (1) type standard: the appearance meets the characteristics of the Luoping red goat, the goat is normal in growth and development, and the breeding goat or the sib doesn't include the Luoping red goat with the genetic defect; (2) initially selecting the breeding goat in four times through a comparison method; (3) once classifying and screening the 12-month goat according to the weight; (4) twice classifying and screening the adult goat according to the weight; (5) classifying and screening the female goat according to the annual output number of lambs; core-field breeding goat matching and grouping: keeping away from proximity of blood, grouping the female goat according to the proportion of 1:20, and breeding by a mating way controlled by a person; according to the breeding goat color and the propagation performance characteristics of the goat in goat selection, creating 3 families including 75 conservation population, wherein each family includes 25 male goats and 500 female goats, and the total number of the male goats is 75 and the total number of the female goats is 1500. According to the necessary management measures for feeding the goat flock, the purification and rejuvenation purpose of the Luoping red goat can be realized.
Owner:罗平县金达生态农业开发有限公司
Propagation method for Huaihai goats
InactiveCN104521878AAchieve multiple birthsStrengthen the bodyAnimal husbandryPregnancyVitamin A Alcohol
The invention provides a propagation method for Huaihai goats. The method comprises the following steps: selecting female goats, which are in an age of 3 to 6 years, have strong bodies and sheeny coat colors, weigh 50 to 60 kg and have produced twins during the first delivery, as breeding goats; and in 2 to 3 weeks before oestrum, centralizedly managing the selected goats, feeding the goats with a concentrated feed everyday and timely cleaning a sheepfold, wherein the fed feed contains 5% of fresh forage and 5% of carrots, a traditional Chinese medicine formula and vitamin A and vitamin E preparations are added into the feed at the same time, and each kg of the feed comprises 50 to 100 g of the traditional Chinese medicine formula, 2 to 3 g of the vitamin A preparation and 3 to 5 g of the vitamin E preparation. According to the invention, experience accumulated in years of breeding of the Huaihai goats is employed, the propagation characteristics of the Huaihai goats are comprehensively taken into consideration, the bodies of the breeding goat are consolidated before delivery through utilization of the traditional Chinese medicine formula and careful feeding before pregnancy, and modern parturition hastening technology is cooperatively utilized, so the Huaihai goats are allowed to give birth to a plurality of baby goats in one delivery and large-scale commercial propagation of the Huaihai goats is made possible.
Owner:陈勇
Primer pairs and kit capable of detecting and distinguishing different breeding sheep babesia
ActiveCN104561344AStrong specificityNo cross reactionMicrobiological testing/measurementMicroorganism based processesBiotechnologySubclinical infection
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Molecular marker capable of simultaneously indicating and identifying spinning count and curvature property of sheep wool and application
ActiveCN107630094AImprove accuracySpeed up the breeding processMicrobiological testing/measurementDNA/RNA fragmentationAnimal scienceExon
The invention relates to the technical field of animal molecular markers and applications, in particular to a molecular marker capable of simultaneously indicating and identifying the spinning count and the curvature property of sheep wool and an application of molecular marker in simultaneously indicating and identifying the spinning count and the curvature property of the wool of Chinese Merinosheep. The molecular marker capable of simultaneously indicating and identifying the spinning count and the curvature property of sheep wool is located at the 23th exon of No.21 chromosome of the FAT3gene. The invention firstly discloses an rs601824181 SNP site which is taken as the molecular marker capable of simultaneously indicating and identifying the spinning count and the curvature propertyof sheep wool. The rs601824181 SNP site is applied as a selection to simultaneously indicate and identify the spinning count and the curvature property of the wool of Chinese Merino sheep, early selection of varieties of breeding sheep with excellent spinning count and curvature property of sheep wool can be performed, and sheep can be selected and remained after being born.
Owner:新疆畜牧科学院畜牧研究所 +1
Sheep hybridization breeding method
The invention discloses a sheep hybridization breeding method. The method comprises the following steps of hybridizing poll Dorset rams with ewes to obtain F1-generation hybrid poll Dorset sheep; hybridizing F1-generation hybrid poll Dorset rams with ewes to obtain F2-generation hybrid poll Dorset sheep; hybridizing F2-generation hybrid poll Dorset ewes with poll Dorset rams to obtain F3-generation hybrid poll Dorset sheep; hybridizing F3-generation poll Dorset sheep with Texel sheep to obtain Songyu sheep. Songyu sheep obtained through the sheep hybridization breeding method are more nutritious than ordinary sheep and meanwhile provide higher mutton yield than the ordinary sheep.
Owner:山西翠泰圣园农产品销售有限公司
Method for resource utilization of agricultural waste feed
InactiveCN101843301AAbundant productionGood acidic tasteFood processingAnimal feeding stuffResource utilizationWater Hyacinths
The invention relates to a method for resource utilization of agricultural waste feed, belonging to the technical field of animal husbandry. The method is as follows: primarily smashing agricultural waste rice straw, water hyacinth growing in non-industrial polluted river channels and ponds, and a by-product vinegar waste in vinegar processing factories; mixing, compacting and sealing according to a specific ratio; and carrying out anaerobic fermentation for 30 days to obtain an ensiling processed-similar fermented feed for feeding sheep. In the method, foreign additives are not needed, fermentation can be carried out under natural conditions, an above average production level when being used for the fattening of growing sheep can be reached, and the adopted processing raw materials are all agricultural wastes, thus providing a new effective way for resource utilization of agricultural waste.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Dorper sheep breeding method
InactiveCN105994146AMeet nutritional needsFeeding method is reasonableFood processingAnimal feeding stuffZoologyGrowing sheep
The invention discloses a Dorper sheep breeding method. The method has the advantages that a breeding mode is reasonable, nutrient requirements of sheep are fully met, good-quality sheep are sorted during breeding sheep selection, and breeding quality is ensured.
Owner:TONGLING DONGSHENG ECOLOGICAL AGRI SCI & TECH CO LTD
Staged feeding management method of black goats
InactiveCN109757430AEasy to manageImprove survival rateAnimal husbandryLactating dairy eweGrowing sheep
The invention discloses a staged feeding management method of black goats. Feeding management means can be adopted according to different growth and development stages. The method specifically comprises the following steps: black goats comprise suckling stage lambs, lactation stage ewes, weaning stage lambs, adolescence stage adult goats and breeding goats according to development stages, the suckling stage lambs and the lactation stage ewes are stocked in corresponding farms according to day ages, and the weaning stage lambs, the adolescence stage adult goats and the breeding goats are stocked in different isolated open pastures or different isolated goat pens; the suckling stage lambs are fed respectively according to day ages, suckling stage lambs 20 days after birth are lactated by ewes and assisted by lamb milk powder or fresh milk, and fresh grass feeding is trained later; goats at different stages are fed respectively with different proportions and types of forage, and accordingly, manual grass planting for corresponding forage is performed in open pastures. The black goats at different stages are fed in a staged manner, so that the survival rate of lambs is high, and muttonis compact.
Owner:蓬安县兴农生态农业开发有限公司
Kit for identifying experimental animal cattle and sheep brucella and detection method thereof
PendingCN107723374ARapid identificationEasy to identifyMicrobiological testing/measurementEscherichia coliCompetent cell
The invention relates to the technical field of bacterial detection and specifically relates to a kit for identifying experimental animal cattle and sheep brucella and a detection method thereof. Thekit comprises PCR reaction liquor, a standard positive template and a negative quality control standard substance, wherein the PCR reaction liquor comprises two pairs of primers for identifying cattleand sheep brucella: upstream brucella melitensis: 5'-CGC TGT CAC TGT TGC AAG TAT G-3'; downstream brucella melitensis: 5'-TCT CGC ATG CGC TAT GAT CT-3'; upstream bovine brucella: 5'-TTG AAG TCT GGC GAG CAT GA-3'; downstream bovine brucella: 5'-TCT CGC ATG CGC TAT GAT CT-3'; the standard positive template comprises a cattle standard positive template and a sheep standard positive template; the cattle standard positive template contains a pMD19-T recombinant plasmid composed of nucleotide fragments with 113 basic groups containing bovine brucella highly conserved genes; the sheep standard positive template contains the pMD19-T recombinant plasmid composed of nucleotide fragments with 128 basic groups containing brucella melitensis highly conserved genes; the two recombinant plasmids are amplified in escherichia coli DH5alpha competent cells.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY +1
Mongolian sheep fecundity improving method based on GDF9 gene coding region mutation
InactiveCN111500744AImprove reproductive performanceMicrobiological testing/measurementDNA/RNA fragmentationNucleotidePhenylalanine
The invention belonging to the field of molecular biology discloses a Mongolian sheep fecundity improving method based on GDF9 gene coding region mutation, thereby providing a molecular marker for improving the double-lamb rate of the Mongolian sheep and detecting whether a T-to-C mutation exists at 1040bp at the downstream of a promoter of a GDF9 gene coding region in a to-be-detected Mongolian sheep genome or not. The site mutation causes the change of the amino acid corresponding to the nucleic acid sequence from phenylalanine to serine to determine the genotype of the mongolian sheep individual at the site; a breeding Mongolian sheep is screened through genotype; with DNA sequencing techniques, single nucleotide polymorphism detection is carried out on the GDF9 gene to compare polymorphism of the GDF9 gene in the Mongolian sheep variety, sequencing, comparing and analyzing are carried out on PCR product fragments and a molecular marker related to fecundity is found out; with the molecular marker, DNA sequencing is established to detect a technical system of the genotype, a breeding sheep is selected by utilizing the genotype so as to improve the fecundity of the Mongolian sheep.
Owner:INNER MONGOLIA UNIVERSITY +1
Breeding goat selection method of crossbred Guizhou white goats
The invention discloses a breeding goat selection method of crossbred Guizhou white goats. The method includes the specific steps that Guizhou white goats and Boer white goats are mated to obtain a Boer first-filial generation, a first batch of basic goat flocks and a second batch of basic goat flocks are screened out, the first batch of basic goat flocks and the second batch of basic goat flocks are subjected to cross hybridization to obtain a Boer second-filial generation, the Boer second-filial generation and the Guizhou white goats are mated to obtain a Boer third-filial generation, then the Boer third-filial generation and the Guizhou white goats are mated to obtain the crossbred Guizhou white goats, and breeding goats are selected. The breeding goats, obtained through the method, of the crossbred Guizhou white goats keep the characteristics, such as high lambing rate, high diseases resistance, good skin toughness, tender meat quality, light goaty flavor and good taste, of the Guizhou white goats, have the advantages, like high growth speed and large size, of Boer goats, and can improve breeding economic benefits of farmers.
Owner:务川自治县宏牧羊业有限公司
Epidemic prevention type sheep house for breeding sheep raising
InactiveCN111587800AImprove cleanlinessGuaranteed disinfectionLavatory sanitoryAnimal housingMedicineEngineering
Owner:河南中羊牧业有限公司
Application of Noggin gene as exogenous gene introduced into cashmere goat cells to improve villus fineness
InactiveCN104372026AImprove finenessImprove qualityGenetic engineeringFermentationLentivirusGrowing sheep
The invention discovers that a Noggin gene generates a regulation and control effect on the villus fineness of Liaoning cashmere goat, that is to say, the higher expression quantity of the Noggin gene can promote the expression quantity of KAP7.1 and KAP8.2 genes to be increased, the increase of the genes can reduce the villus fineness and improve the villus quality, and thus an application of the Noggin gene as an exogenous gene introduced into cashmere goat cells to improve the villus fineness is disclosed; Noggin gene over-expressed lentivirus is injected into a male Liaoning cashmere goat testis, and after the KAP26.1 gene as an exogenous gene is introduced into the cashmere goat cells through virus injection, a Liaoning cashmere goat individual having high expression quantity of the KAP7.1 and KAP8.2 genes is selected and bred as a breeding sheep, so that the villus fineness of the cashmere goat can be improved, and the villus quality is increased.
Owner:LIAONING NORMAL UNIVERSITY
Automatic water drinking equipment for breeding sheep
InactiveCN111567432ASimple structureIncrease laborAnimal watering devicesHollow article cleaningCrop livestockFishery
The invention discloses automatic water drinking equipment for breeding sheep. The equipment comprises a sheep house side wall and a water trough, wherein the water trough is arranged on the inner side wall surface of the sheep house side wall, an automatic water supply mechanism is arranged on the outer side wall surface of the sheep house side wall, one end of the automatic water supply mechanism communicates with the water trough, and a movable cleaning mechanism is arranged on one side of the water trough. An existing water trough is improved; the automatic water supply mechanism is arranged on one side of the water trough, so that water can be supplemented into the water trough regularly and quantitatively; meanwhile, the movable cleaning mechanism is arranged on the upper portion ofthe water trough, so that after the interior of the water trough is stained, the water trough can be cleaned through the movable cleaning mechanism; and the equipment is simple and convenient to operate, and solves the problems that in the prior art, most livestock drinking devices are relatively simple in structure, the labor amount of workers is greatly increased as manual water adding is needed, and the water trough is located in a sheepfold, so that water in the water trough is likely to be stained by sheep, and cleaning is troublesome.
Owner:河南中羊牧业有限公司
Method for breeding improved mutton sheep strain
ActiveCN102550485BOvercoming heterogeneityIn line with breeding goalsAnimal husbandryGermplasmGrowing sheep
Owner:天津奥群牧业有限公司 +1
Dazu black goat breeding and variety protection method
ActiveCN112568186AImprove reproductive performanceImprove meat qualityFood processingAnimal husbandryFisheryZoology
The invention relates to the technical field of goat breeding and variety protection, and discloses a Dazu black goat breeding and variety protection method. The Dazu black goat breeding and variety protection method comprises the following steps of 1) core group building, 2) breeding, 3) inbreeding coefficient control and 4) breeding target. According to the Dazu black goat breeding and variety protection method, excellent individuals are selected in a Dazu black goat production area to establish a core breeding group, so that excellent characteristics such as appearance, body shape, high reproductive performance, meat quality and stress (disease) resistance of big-foot black goats are kept; and through purebred comprehensive breeding generation by generation, consistency of body shape and appearance is improved, growth speed and meat production performance are improved, breeding value and economic value are continuously improved, finally, a Dazu black goat breeding system is furtherperfected under radiation driving of the core group, formation and development of the Dazu black goat breeding industry are promoted, and the purposes of purification, rejuvenation, breeding efficiency improvement and the like are achieved. The method effectively solves the problem that at present, the breeding structure of Dazu black goats is unreasonable.
Owner:重庆腾达牧业股份有限公司
Method for resource utilization of agricultural waste feed
InactiveCN101843301BAbundant productionGood acidic tasteFood processingAnimal feeding stuffResource utilizationAnimal husbandry
Owner:JIANGSU ACAD OF AGRI SCI
Straw feed formula with Chinese herbal medicine additives for breeding sheep
InactiveCN106509406ARich varietyImprove developmentAnimal feeding stuffAccessory food factorsBiotechnologyNormal growth
The invention discloses a straw feed formula with Chinese herbal medicine additives for breeding sheep. The straw feed comprises the following components in parts by weight: 85-105 parts of wheat, 20-28 parts of alfalfa, 15-20 parts of straw, 8-10 parts of pine needle powder, 3-4 parts of fennel seeds, 5-6 parts of echinacea purpurea, 4-5 parts of walnut kernels, 2-3 parts of calcium powder, 7-8 parts of beets, 5-6 parts of sugarcane powder, 1-2 parts of traditional Chinese medicine additives, an appropriate amount of edible salt, and 0.2-0.5 part of an attractant. The components are all purely natural raw materials which are free of any chemical drugs; moreover, the raw materials are rich in spices and full in nutrition. The added traditional Chinese medicine additives contain active ingredients of natural medicines, and are capable of promoting reproductive system development of the rams. The straw feed further contains multiple nutrients, so that the straw feed is capable of ensuring normal growth and development of the rams.
Owner:重庆市开州区钦梅农牧有限公司
Colloidal gold detection test strip for detecting Brucella antigen by sandwich method
InactiveCN113092755AEliminate distractionsIncreased sensitivityBiological testingImmunoassaysBrucella antigenEscherichia coli
The invention relates to a colloidal gold detection test strip for detecting a Brucella antigen by a sandwich method. The detection test strip is composed of a Brucella antigen detection test strip and a plastic suction head, and the detection test strip is composed of a PVC base plate, a sample pad, a gold label pad, a nitrocellulose membrane and a water absorption pad. A four-strain Brucella monoclonal antibody sandwich method combined with different antigen epitopes is adopted, a colloidal gold labeled Brucella monoclonal antibody 4D7 is coated on a gold label pad, a mixed solution of Brucella monoclonal antibodies 2B5, 5F3 and 6C2 is coated on a detection line, and a goat anti-mouse IgG antibody is coated on a quality control line. The test strip for detecting the Brucella antigen can be used for rapidly detecting bovine breed, sheep breed and swine breed Brucella on site and effectively eliminating interference of enterocolitis yersinia O9 and escherichia coli O157 on Brucella antigen detection. The test strip is high in sensitivity and sensitivity, good in specificity, simple and convenient to use, applicable to detection of samples such as clinical tissues, environments and milk, and also applicable to identification of purely cultured Brucella.
Owner:CHINA INST OF VETERINARY DRUG CONTROL
Primer and kit for identifying four brucella in dog and application of primer and kit
PendingCN111500754AHigh detection specificityHigh sensitivityMicrobiological testing/measurementMicroorganism based processesDimerBrucella suis
The invention belongs to the technical field of molecular biology, and relates to a single PCR detection primer, kit and method for four brucella (brucella melitensis, brucella bovis, brucella suis and brucella canis) capable of infecting dogs. The rough brucella (dog brucella lacking 357bp) and smooth brucella (cattle breed, sheep breed and pig breed) can be respectively amplified by 352 bp and 709 bp just by only one pair of primers, so that effective identification can be carried out. In addition, the primers can effectively avoid the generation of primer dimers and hairpin structures, ensure the efficient PCR amplification and enhance the specificity and sensitivity of the detection technology.
Owner:山东省健牧生物药业有限公司
Molecular marker influencing cashmere fiber diameter character of southern Xinjiang cashmere goat as well as specific primer pair and application of molecular marker
PendingCN114250309AImprove accuracySpeed up the breeding processMicrobiological testing/measurementDNA/RNA fragmentationNucleotideFishery
The invention relates to the technical field of animal molecular markers, in particular to a molecular marker for influencing the diameter character of cashmere fibers of southern Xinjiang cashmere goats and a specific primer pair and application of the molecular marker for influencing the diameter character of the cashmere fibers of the southern Xinjiang cashmere goats, and the molecular marker for influencing the diameter character of the cashmere fibers of the southern Xinjiang cashmere goats is located on a KRTAP24-1 gene of a No.1 chromosome of the southern Xinjiang cashmere goats. The KRTAP24-1 gene is located at the 4033765bp-4034298bp position of the nucleotide sequence of the chromosome 1, the molecular marker is located at the 4034201bp position of the nucleotide sequence of the chromosome 1, and a mutant basic group is C or T. The molecular marker disclosed by the invention can be used for realizing early-stage breeding of breeding sheep with excellent cashmere fiber diameter characters, and the breeding sheep can be selected and remained after being born, so that the breeding cost is reduced, the breeding process of high-quality superfine southern Xinjiang cashmere goats is accelerated, and a theoretical basis and guidance are provided for genetic improvement of the southern Xinjiang cashmere goats.
Owner:INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI +1
Kit for identifying brucella abortus and other brucella species
ActiveCN113637056AMicrobiological testing/measurementClimate change adaptationBrucella speciesTGE VACCINE
The invention provides a kit for identifying brucella abortus and other brucella species. The kit is coated with a protein as shown in SEQ ID NO. 2 and a brucella BP26 protein. The invention finds that the protein as shown in SEQ ID NO.2 or the coding gene thereof can be used for distinguishing the brucella abortus from other brucella species. The invention provides an iELISA detection reagent for identifying brucella abortus infection serum and other brucella infection serum, and the reagent contains the two proteins, or contains recombinant proteins with His tags fused at the N ends or C ends of the two proteins. The iELISA detection reagent provided by the invention can quickly identify antibodies of bovine vaccine immunization or wild viruses and other species (sheep, pigs, dogs and the like) vaccine immunization or wild viruses in livestock serum, solves the problem of difficult identification of Brucella strain types in livestock groups, and provides reference for pathogen identification, vaccine selection and livestock group purification of livestock brucellosis.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY
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