Mongolian sheep fecundity improving method based on GDF9 gene coding region mutation

Abstract

The invention belonging to the field of molecular biology discloses a Mongolian sheep fecundity improving method based on GDF9 gene coding region mutation, thereby providing a molecular marker for improving the double-lamb rate of the Mongolian sheep and detecting whether a T-to-C mutation exists at 1040bp at the downstream of a promoter of a GDF9 gene coding region in a to-be-detected Mongolian sheep genome or not. The site mutation causes the change of the amino acid corresponding to the nucleic acid sequence from phenylalanine to serine to determine the genotype of the mongolian sheep individual at the site; a breeding Mongolian sheep is screened through genotype; with DNA sequencing techniques, single nucleotide polymorphism detection is carried out on the GDF9 gene to compare polymorphism of the GDF9 gene in the Mongolian sheep variety, sequencing, comparing and analyzing are carried out on PCR product fragments and a molecular marker related to fecundity is found out; with the molecular marker, DNA sequencing is established to detect a technical system of the genotype, a breeding sheep is selected by utilizing the genotype so as to improve the fecundity of the Mongolian sheep.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a method for improving the fecundity of Mongolian sheep based on the mutation of the GDF9 gene coding region. Background technique [0002] GDF9 (growth differentiation factor 9, GDF9) is growth differentiation factor 9, and BMP15 gene belongs to transforming growth factor β (TGFβ) superfamily members. The GDF9 gene of sheep is located on chromosome 5, with a total length of about 2.5kb, which contains 2 exons and 1 intron. Exon 1 is 397bp long, encoding 134 amino acids, and exon 2 is 968bp long. It encodes 456 amino acids, and the intron is 1126bp long. [0003] Mongolian sheep is the largest and most widely distributed coarse-wool sheep breed in China. Native to the Mongolian Plateau. It is now distributed in Inner Mongolia, Northeast China, North China and Northwest China. Typical low-level populations include Ujumqin sheep and Sunite sheep in Xilin Gol, small-tailed Mongo...

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Problems solved by technology

[0008] Guan Feng et al. used PCR-RFLP method to study GDF9 gene G8 mutation in Hu sheep, Charolais, Dorset, Suffolk, Chinese Merino meat multi-fetal lines, Chinese Merino sheep and Romney 7 sheep The distribution in the population, it was found that the G8 homozygous mutation was only detected in the Hu sheep population, but the incidence rate was extremely low (0.645%, 2 / 310)

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