Molecular marker influencing cashmere fiber diameter character of southern Xinjiang cashmere goat as well as specific primer pair and application of molecular marker
A cashmere fiber and molecular marker technology, applied in biochemical equipment and methods, microbe determination/testing, DNA/RNA fragments, etc.
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Embodiment 1
[0033] Example 1: The molecular marker (SNP28 site of the present invention) that affects the cashmere fiber diameter traits of southern Xinjiang cashmere goats is located on the KRTAP24-1 gene of chromosome 1 of southern Xinjiang cashmere goats, and the KRTAP24-1 gene is located at No. 1 At the 4033765bp-4034298bp of the chromosome nucleotide sequence, the molecular marker is located at the 4034201bp of the chromosome 1 nucleotide sequence, and the mutation base is C or T.
Embodiment 2
[0034] Embodiment 2: A specific primer pair for amplifying the molecular markers affecting the cashmere fiber diameter traits of southern Xinjiang cashmere goats described in Example 1, characterized in that it includes upstream primers and downstream primers,
[0035]Upstream primer F (as shown in the sequence table SEQ ID No: 1):
[0036] 5'-tggtgtgacatagaccaaagagcta-3';
[0037] Downstream primer R (as shown in the sequence table SEQ ID No: 2):
[0038] 5'-ctcttggataagcagttaagttgcc-3'.
Embodiment 3
[0039] Example 3: The application of a pair of specific primers described in Example 2 in the preparation of a reagent or kit for in vitro detection of molecular markers that affect the cashmere fiber diameter traits of Xinjiang cashmere goats.
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