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Molecular marker influencing cashmere fiber diameter character of southern Xinjiang cashmere goat as well as specific primer pair and application of molecular marker

A cashmere fiber and molecular marker technology, applied in biochemical equipment and methods, microbe determination/testing, DNA/RNA fragments, etc.

Pending Publication Date: 2022-03-29
INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the approval of new breeds of cashmere goats in southern Xinjiang, there are few reports on cashmere traits.

Method used

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  • Molecular marker influencing cashmere fiber diameter character of southern Xinjiang cashmere goat as well as specific primer pair and application of molecular marker
  • Molecular marker influencing cashmere fiber diameter character of southern Xinjiang cashmere goat as well as specific primer pair and application of molecular marker
  • Molecular marker influencing cashmere fiber diameter character of southern Xinjiang cashmere goat as well as specific primer pair and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: The molecular marker (SNP28 site of the present invention) that affects the cashmere fiber diameter traits of southern Xinjiang cashmere goats is located on the KRTAP24-1 gene of chromosome 1 of southern Xinjiang cashmere goats, and the KRTAP24-1 gene is located at No. 1 At the 4033765bp-4034298bp of the chromosome nucleotide sequence, the molecular marker is located at the 4034201bp of the chromosome 1 nucleotide sequence, and the mutation base is C or T.

Embodiment 2

[0034] Embodiment 2: A specific primer pair for amplifying the molecular markers affecting the cashmere fiber diameter traits of southern Xinjiang cashmere goats described in Example 1, characterized in that it includes upstream primers and downstream primers,

[0035]Upstream primer F (as shown in the sequence table SEQ ID No: 1):

[0036] 5'-tggtgtgacatagaccaaagagcta-3';

[0037] Downstream primer R (as shown in the sequence table SEQ ID No: 2):

[0038] 5'-ctcttggataagcagttaagttgcc-3'.

Embodiment 3

[0039] Example 3: The application of a pair of specific primers described in Example 2 in the preparation of a reagent or kit for in vitro detection of molecular markers that affect the cashmere fiber diameter traits of Xinjiang cashmere goats.

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Abstract

The invention relates to the technical field of animal molecular markers, in particular to a molecular marker for influencing the diameter character of cashmere fibers of southern Xinjiang cashmere goats and a specific primer pair and application of the molecular marker for influencing the diameter character of the cashmere fibers of the southern Xinjiang cashmere goats, and the molecular marker for influencing the diameter character of the cashmere fibers of the southern Xinjiang cashmere goats is located on a KRTAP24-1 gene of a No.1 chromosome of the southern Xinjiang cashmere goats. The KRTAP24-1 gene is located at the 4033765bp-4034298bp position of the nucleotide sequence of the chromosome 1, the molecular marker is located at the 4034201bp position of the nucleotide sequence of the chromosome 1, and a mutant basic group is C or T. The molecular marker disclosed by the invention can be used for realizing early-stage breeding of breeding sheep with excellent cashmere fiber diameter characters, and the breeding sheep can be selected and remained after being born, so that the breeding cost is reduced, the breeding process of high-quality superfine southern Xinjiang cashmere goats is accelerated, and a theoretical basis and guidance are provided for genetic improvement of the southern Xinjiang cashmere goats.

Description

technical field [0001] The invention relates to the technical field of animal molecular markers, and relates to a molecular marker that affects the diameter properties of cashmere fibers of Xinjiang cashmere goats and its specific primer pair and application thereof. The application of the molecular marker reagent or kit for the diameter trait, and the application of the molecular marker in the selection of the cashmere fiber diameter trait of the southern cashmere goat. Background technique [0002] Cashmere is the main product of cashmere goats. It is soft and stretchable. Its fabrics are close-fitting, comfortable, sweat-absorbing, warm-keeping, and breathable. It is considered a special animal fiber as a high-grade textile raw material by contemporary people. Wearing cashmere clothing shows luxury, elegant style and smooth feel. [0003] The fineness of cashmere is the most important indicator of cashmere quality. The light weight of cashmere fabrics and the flexibility...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156Y02P60/87
Inventor 田可川吴翠玲付雪峰刘桂芬张果平魏晨秦崇凯石刚薛多雄何军敏刘静毛静艺
Owner INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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