Brassica napus cytoplasmic male sterile restore line population improvement method

Abstract

The invention provides a brassica napus cytoplasmic male sterile restore line population improvement method. The method comprises the following steps: a restore line is taken as a female parent and is hybridized with a plurality of inbred lines, a first filial generation selects a fertile plant as a female parent for backcross and inbred for a generation, restore gene locus homozygous single plants are selected through identification, equivalent seeds are taken from each single plant and mixed to form an S0 population, the S0 population is sown, a chemical inducer is sprayed to the female parent, open pollination is carried out in the population to harvest chemically-induced sterile plant seeds to form a recurrent selection population S1, then recurrent selection is carried out for at least 6 times, and a DH single plant obtained by carrying out field planting and microspore culture on a screened target single plant is a brassica napus cytoplasmic male sterile restore line. According to the method, a chemical induction method is initiatively adopted to acquire a sterile plant, so that the time and the labor are saved, and finally, the genetic basis of the restore line is wide; and more importantly, in the process of applying a chemically-induced sterile plant to carry out recurrent selection, the ratio of restore genes in the recurrent population is stable to be 100 percent.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and in particular relates to a method for improving a population of a cytoplasmic sterile restorer line of Brassica napus based on chemically induced male sterility lines. Background technique [0002] Rapeseed is one of the most important oil crops in the world. A large number of studies at home and abroad have shown that heterosis among rapeseed varieties is significant, and male sterility is one of the important ways to utilize heterosis in rapeseed. At present, polima and radish cytoplasmic male CMS line realizes heterosis utilization in rapeseed. Restorer line selection is the basis for the successful application of these two types of sterile lines. The existing restorer line selection methods mainly include backcrossing and recurrent selection. [0003] For example, Chinese patent document CN1466870A discloses a method for breeding cytoplasmic male sterile restorer lines of rapes...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the disadvantages of the existing rapeseed cytoplasmic sterile restorer line selection method, such as the single genetic material of the basic material, the time-consuming and labor-intensive creation of the basic population, and the insufficient gene recombination during the establishment period of the selection population. Provides a population improvement method for cytoplasmic sterile restorer lines of Brassica napus that saves time and effort, has a broad material genetic base, and maintains a 100% ratio of restorer genes in the population