44 results about "Puccinia graminis tritici" patented technology

The invention belongs to the field of gene engineering, and discloses a gene TaFBK1 with F-box structure field, and an expression vector and application thereof. The cDNA (complementary deoxyribonucleic acid) sequence of the gene TaFBK1 with F-box structure field is SEQ ID NO.1, and the coded amino acid sequence is SEQ ID NO.2. The gene is from common wheat (Triticum asetivum L.) 92R137. The TaFBK1 is induced by Puccinia strilformis to enhance the expression in anti-Puccinia-strilformis wheat species 92R137, and the expression level is much higher than that in the Puccinia-strilformis-infected wheat species Yangmai 158. When the gene is inserted into pBI220 to obtain an overexpression vector and used for transforming the Puccinia-strilformis-infected wheat species Yangmai 158, the T1-generation anti-Puccinia- strilformis identification result on the positive transformed plant indicates that the overexpression of the TaFBK1 can enhance the Puccinia strilformis resistance of the Puccinia-strilformis-infected wheat species.

The invention relates to the field of pesticide compounding techniques, particularly relates to a penconazole and zhongshengmycin-containing sterilization composition, and also relates to an application thereof. The penconazole and zhongshengmycin-containing sterilization composition comprises effective ingredients of penconazole and zhongshengmycin with a weight part ratio of (90:1)-(1:90), and the penconazole and zhongshengmycin-containing sterilization composition is prepared into a wettable powder, a suspending agent, an emulsion in water, a water dispersible granule, emulsifiable concentrate and the like after being added with assistants and an excipient, and is used for controlling fungal diseases caused by germs such as erysiphe, uromyces, mortierella, septoria and venturia and bacterial diseases caused by germs such as Gram-positive bacteria, Gram-negative bacteria, mycobacteria and saccharomycetes. The penconazole and zhongshengmycin-containing sterilization composition has a relatively good synergism within a certain range, is superior to a single preparation on the aspect of control effects, is relatively durable in pesticide effect, has a special effect on controlling powdery mildew, rust diseases, scab, leaf spot diseases, gray mold, white rot, soft rot, streak diseases, rice blast, ring spot, gibberellic diseases and bacterial diseases, is relatively low in residue, is relatively easily decomposed after a lasting period, is relatively environment-friendly, and is beneficial to the increase of yield and income in an agricultural district.

The present invention relates to a method of increasing resistance against fungal pathogens of the order Pucciniales, preferably the family Phacopsoraceae, in plants and / or plant cells. This is achieved by increasing the expression of an HCP5 protein or fragment thereof in a plant, plant part and / or plant cell in comparison to wild type plants, wild type plant parts and / or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and / or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the order Pucciniales, preferably the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding an HCP5 protein.

The invention provides a colloidal gold test strip used for detecting wheat leaf rust. The colloidal gold test strip comprises a sample pad, a colloidal-gold pad, an NC membrane, absorbent paper and a PS base plate, wherein the sample pad, the colloidal-gold pad, the NC membrane and the absorbent paper are successively arranged on the PS base plate along the chromatographic direction of a sample, the colloidal-gold pad is coated by a colloidal gold-labeled monoclonal antibody against Puccinia triticina, the NC membrane successively comprises a detection line and a quality control line along the chromatographic direction of the sample, the detection line is coated with Puccinia triticina antigen protein that the monoclonal antibody can specifically recognize, and the quality control line is coated with a goat anti-mouse secondary antibody. The monoclonal antibody against Puccinia triticina is secreted by a hybridoma cell with an accession number of CGMCC No. 10884. When the test strip is used for detection, the process from sampling to obtainment of detection results only takes 15 min, operation is simple and convenient, the results are accurate, early warning of wheat leaf rust can be realized, and economic loss caused by diseases is reduced.

The invention relates to the field of pesticide compounding techniques, particularly relates to a penconazole and zhongshengmycin-containing sterilization composition, and also relates to an application thereof. The penconazole and zhongshengmycin-containing sterilization composition comprises effective ingredients of penconazole and zhongshengmycin with a weight part ratio of (90:1)-(1:90), and the penconazole and zhongshengmycin-containing sterilization composition is prepared into a wettable powder, a suspending agent, an emulsion in water, a water dispersible granule, emulsifiable concentrate and the like after being added with assistants and an excipient, and is used for controlling fungal diseases caused by germs such as erysiphe, uromyces, mortierella, septoria and venturia and bacterial diseases caused by germs such as Gram-positive bacteria, Gram-negative bacteria, mycobacteria and saccharomycetes. The penconazole and zhongshengmycin-containing sterilization composition has a relatively good synergism within a certain range, is superior to a single preparation on the aspect of control effects, is relatively durable in pesticide effect, has a special effect on controlling powdery mildew, rust diseases, scab, leaf spot diseases, gray mold, white rot, soft rot, streak diseases, rice blast, ring spot, gibberellic diseases and bacterial diseases, is relatively low in residue, is relatively easily decomposed after a lasting period, is relatively environment-friendly, and is beneficial to the increase of yield and income in an agricultural district.

The present invention relates to a method of increasing resistance against fungal pathogens of the order Pucciniales in plants and / or plant cells. This is achieved by increasing the expression of a MybTF protein or fragment thereof in a plant, plant part and / or plant cell in comparison to wild type plants, wild type plant parts and / or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and / or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the order Pucciniales, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding a MybTF protein.

The invention discloses 'a monoclonal antibody, an immune fluorescence method and a kit for detecting puccinia striiformis f.sp.tritici, puccinia triticina f.sp.tritici and puccicinia graminis f.sp.tritici' and belongs to the field of agricultural biotechnology. The invention mainly provides the monoclonal antibody for detecting puccinia striiformis f.sp.tritici, puccinia triticina f.sp.tritici and puccicinia graminis f.sp.tritici, which is secreted by hybridoma cell line with a collection number CGMCC No.4414 and can identify puccinia triticina f.sp.tritici, puccicinia graminis f.sp.tritici and puccinia striiformis f.sp.tritici at the same time. The invention also provides a detection method based on the combination of the monoclonal antibody and an immune fluorescence technology and a kit. The monoclonal antibody, the detection method and the kit have the advantages of high specificity, high sensitivity, and simplicity and quickness of operation.

The invention belongs to the technical field of gene engineering, and discloses mannose-1-phosphate guanylyl transferase, a coding gene and application. The amino acid sequence of the mannose-1-phosphate guanylyl transferase is SEQIDNO: 1; the nucleotide sequence of the DNA for coding the mannose-1-phosphate guanylyl transferase is as shown in SEQ ID NO: 2; and the cDNA (complementary deoxyribonucleic acid) nucleotide sequence of the mannose-1-phosphate guanylyl transferase is shown as SEQ ID NO: 3. The mannose-1-phosphate guanylyl transferase PsMT1 provided by the invention is a hexose transport protein family member, has a mannose transport function, and plays an important role in a process of catalyzing conversion of fructose into mannose; and the PsMT1 gene expression is inhibited through a gene engineering technology to influence the thallus growth of wheat stripe rust (Puccinia striiformis), the pathogenicity of wheat stripe rust is reduced, and the gene has a good application prospect in the aspect of developing small molecule nucleotide pesticides for preventing and treating wheat stripe rust.

The invention provides a method for researching genetic and propagation paths of a corn southern rust group based on an SNP (Single Nucleotide Polymorphism) marker. According to the method, DNA of different strains is subjected to enzyme digestion by using screened restriction enzymes EcoRI and MspI, so that a genome sequencing technology is simplified, SNP markers are mined by using Stacks software, and then the genetic structure and the propagation path of a puccinia multiflora population are researched; the restriction endonucleases EcoRI and MspI provided by the invention have a better cutting effect on the whole genome of puccinia multiflora, and compared with other combinations, more tags can be obtained under the condition of the same sequencing depth. At present, whole genome sequencing of puccinia multiflora is not performed yet, and the method for researching the population genetic structure and the propagation path of puccinia multiflora on the basis of the SNP sites obtained by simplifying genome sequencing and utilizing stacks software has important significance on comprehensive treatment and accurate prevention and control of diseases.

The invention discloses a method for predicting Apocynum rust caused by Apocynum palisander and a special device for the method. Disease force calculation steps are realized. It can predict more accurately the occurrence of grid rust in field planting of Apocynum apocynum, and then effectively formulate the prevention and control plan. Compared with the prior art, the present invention solves the problem of effectively measuring the number of pathogenic bacteria with infecting ability while measuring the number of pathogenic bacteria, especially solving the problem that the air microbial sampler completes the sampling of the detached leaves of Apocynum apocynum in vitro. The problem of pollution and rot is suitable for the prediction and forecasting of grid rust in field commercial plantation of apocynum.

The invention provides application of a puccinia striiformis (PSTG_17694) gene in prevention and treatment of puccinia striiformis. The sequence of the PSTG_17694 gene is shown in Seq ID No.1; the PSTG_17694 gene can effectively regulate and control growth and propagation of puccinia striiformis and is used as a molecular target of transcriptional regulation and control or used as a gene target for inhibiting a protein function, and the PSTG_17694 gene is silenced to achieve the effect of inhibiting the growth and propagation of puccinia striiformis. According to a cultivation method for anti-puccinia-striiformis wheat by using the gene, the obtained wheat has obvious puccinia striiformis resistance.

The invention provides application of a puccinia striiformis PSTG_06371 gene to prevention and control of wheat stripe rust. The sequence of the PSTG_06371 gene is shown as SEQ ID No. 5; the PSTG_06371 gene can be used for effectively regulating and controlling growth and proliferation of puccinia striiformis; the PSTG_06371 gene is used as a molecular target for transcriptional regulation or a gene target for inhibiting a protein function; the effect of inhibiting the growth and proliferation of the puccinia striiformis is realized through silencing the PSTG_06371 gene. Wheat obtained by themethod for culturing puccinia striiformis resisting wheat by utilizing the gene has remarkable puccinia striiformis resistance.

The invention discloses the preparation and application of a multigene pedigree screening database of the Cylindricaceae. The present invention provides the application of the substances shown in a) and b) in identifying or distinguishing the species of Cylindricaceae, and the substance shown in a) is a substance for detecting the following gene sequence in the sample to be tested or the pathogenic bacteria to be tested: ITS and LSU; the material shown in b) is the ITS and LSU gene sequences in the existing Cylindropyraceae species and various genus; the present invention uses the ITS and LSU gene sequences to carry out the construction of the evolutionary tree and the homology comparison, which can Detecting Pillarphyllaceae, at the quarantine port, can quickly and accurately screen samples containing Pillarphyllaceae fungi to prevent the spread of germs along with the import, export and transportation of seeds and other reproductive materials, so as to protect the healthy development of agricultural production in our country , is of great significance.