Transcription factor for improving rust disease resistant wheat varieties and screening and obtaining method of transcription factor

Abstract

The invention belongs to the technical field of crop variety breeding, and discloses a transcription factor for improving rust disease resistant wheat varieties and a screening and obtaining method ofthe transcription factor. The transcription factor is TaWRKY19 and BdWRKY67 of a WRKY transcription factor in brachypodium distachyon, wherein the ORF coding sequence of the TaWRKY19 is SEQID NO:1, and the ORF coding sequence of the BdWRKY67 of the WRKY transcription factor gene in the brachypodium distachyon is SEQID NO: 2. An RNAi mutant of the WRKY transcription factor is obtained through agrobacterium tumefaciens mediated heredity and transformation, brachypodium sylvaticum is inoculated for disease resistance identification, and through the result, the inventor finds that the RNAi plantdisease resistance of the BdWRKY67 is notably improved. The disease resistance of plant wheat obtained through silencing of the TaWRKY19 transcription factor is notably improved, and the resistance ofplants subjected to instantaneous overexpression to puccinia striiformis is also obviously diminished, so that the transcription factor can be used for heredity improvement of stripe rust resistant wheat.

Description

Technical field [0001] The invention belongs to the technical field of crop variety breeding, and in particular relates to a transcription factor used for the improvement of wheat rust resistant varieties and a method for screening and obtaining the same. Background technique [0002] At present, the closest existing technology: the most commonly used method to respond to pathogenic bacteria formed in the long-term evolution of host plants is to regulate the expression level of genes related to pathogenic genes. Transcription factors (Transcription factors) are a family of genes that are widely present in plants that can increase transcriptional regulation or transcriptional suppression genes in response to related TFs, so that host plants can resist the infestation of pathogens. Living vegetative parasitic fungi include Puccinia sp., Blumeria graminis and Ustilago maydis. They are the three major living vegetative pathogenic fungi in the world and often cause serious damage to t...

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Problems solved by technology

[0005] (1) Wheat has a large and complex genome, low transformation efficiency (TE) and poor genetic transformation. It is urgent to find a plant that can be used as a wheat model plant with high efficiency, easy cultivation, and infection of wheat and can be infected by most pathogenic bacteria - Brachypodium distachyon

[0006] (2) The discovery and utilization of wheat disease resistance genes is the most economical and effective measure to improve wheat crops. It takes a long time to screen using traditional genetic improvement methods. How to quickly discover disease resistance and its regulatory genes is facing a major challenge. challenge

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