Acquisition of edited wheat susceptible gene plants and application of edited wheat susceptible gene plants in disease-resistant variety cultivation

Abstract

The invention belongs to the technical field of disease-resistant variety cultivation, and discloses an acquisition of edited wheat susceptible gene plants and application of edited wheat susceptiblegene plants in disease-resistant variety cultivation. The edited wheat susceptible gene plants have B, D and A copies in wheat. The RNAi mutants of the TaRIPK1 with two genetic backgrounds are obtained. According to the invention, an agrobacterium tumefaciens-mediated genetic transformation method is adopted, and a CRISPR-Cas9 gene editing technology is utilized to obtain the gene edited plant ofTaRIPK1. Through inoculation of wheat stripe rust bacteria, it is found that TaRIPK1 RNAi plant bacteria with two genetic backgrounds have remarkable resistance to stripe rust bacteria toxic race CYR32, disease resistance identification is conducted on a TaRIPK1 gene editing technology, and it is found through results that the disease resistance of TaRIPK1 gene editing plants is remarkably improved. The resistance to main epidemic races of puccinia striiformis and puccinia leaf rust is remarkable, and the plants can be used as a germplasm new material for genetic improvement of wheat rust resistance.

Description

technical field [0001] The invention belongs to the technical field of breeding disease-resistant varieties, and in particular relates to the acquisition of edited wheat disease-susceptible gene plants and its application in breeding disease-resistant varieties. Background technique [0002] Currently, wheat is one of the most widely grown cereal crops, providing the staple food for more than 2.5 billion people worldwide (http: / / www.fao.org / faostat / ). Wheat rust caused by three kinds of rust fungi, stripe rust, stem rust and leaf rust, is the most notorious disease in wheat production (McIntosh et al., 1995; Ellis et al., 2014). It occurs and is prevalent worldwide, causing severe yield loss and seriously threatening wheat production and safety (Chen et al., 2014; Park et al., 2015; Han and Kang., 2018). Planting disease-resistant varieties is the most economical, effective, and environmentally friendly strategy to prevent wheat rust. Although breeders have developed a lar...

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Problems solved by technology

[0007] (3) Although the editing of S gene mutants can achieve broad-spectrum resistance of crops to diseases, most of these mutants will over-consume all the nutrients in the process of plant growth and development under the potential of disease resistance. disease and sustaining wheat material?

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