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A kind of animal growth promoter containing Puccinia reedi and its preparation method

A technology of animal growth and accelerator, applied in the field of development and research of feed additives, can solve the problems of drug resistance drug residues, imbalance of normal gastrointestinal flora, lower immune function level, etc., and achieve the effect of promoting growth

Active Publication Date: 2022-05-03
VETERINARY INST XINJINAG ACADEMY OF ANIMAL SCI CLINIC MEDICAL SCI RES CENT XINJIANG ACADEMY OF ANIMAL HUSBANDRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the 1940s, with the widespread application of antibiotics in the field of feeding, people gradually realized the disadvantages of extensive use of antibiotics, such as causing an imbalance in the normal flora of the gastrointestinal tract, reducing the level of immune function, producing drug resistance and drug residues and threatening human health etc.

Method used

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  • A kind of animal growth promoter containing Puccinia reedi and its preparation method
  • A kind of animal growth promoter containing Puccinia reedi and its preparation method
  • A kind of animal growth promoter containing Puccinia reedi and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, the characteristic of target Puccinia reedi

[0017] Teliospore morphological characteristics: the teliospores of Puccinia reed that the present invention relates to have following characteristics: teliospores pile up on both sides of blade and live, take leaf back as main, be dark brown, heal into sheet mattress shape, teliospore oblong, ellipse Shaped, rounded at both ends, yellowish brown, size (42.5-57.5) μm × (20-32.5) μm, thin wall (2.5-5.0) μm, top wall 5.0 μm, such as figure 1 . The 18S rDNA sequence of Puccinia reedi is shown in SEQ ID NO.1.

Embodiment 2

[0018] Embodiment 2, the preparation method that contains the growth promoter of Puccinia reedi

[0019] 1. Collection of teliospores of Puccinia reeds: From November to April of the following year, the leaves of reeds infected by Puccinia reeds were collected, and the teliospores of Puccinia reeds on the leaves healed into a mattress-like sheet, which was dark brown; 2. Drying: The collected reed leaves are spread out and dried naturally or dried, so that the water content is less than <3%; 3. Reed leaf crushing: use a 20-mesh crusher to crush the dried reed leaves, and repeatedly crush for 3-5 More than one time, after crushing, pass through a 40-mesh sieve, and discard particles larger than 40 mesh; 4. Broken Puccinia reed teliospores: Soak the teliospores of Puccinia reed filtered through a 40-mesh sieve with urban tap water or other potable water , mix well, 10-500g / L, use the bead milling method to crush the teliospores, the diameter of the grinding beads is 0.5-3.0mm, a...

Embodiment 3

[0022] Example 3. When preparing animal feed or concentrate, add the growth promoter containing Puccinia reedi prepared in Example 2 in a ratio of 0.1% to 3%, mix well, and feed.

[0023] mouse feeding test

[0024] According to the ratio of 0, 0.1%, 1% and 3%, the prepared growth promoters were added to the complete diet of mice (prepared by the Experimental Animal Training Center of Xinjiang Medical University), prepared into pellet feed, and cleaned according to the following table Kunming mouse animal feeding.

[0025] feeding design

[0026]

[0027] Note: The feed intake of adult mice is generally 3-7 grams / day, and that of young mice is generally 1-3 grams / day

[0028] Feeding results show: Group 3, mice were fed for 35 days in the state of overnutrition (6 g / mouse / day), and the daily gain of the mice in the growth promoter adding group was greater than that of the control group. Compared with the control group, 0.1%, 0.1%, The daily weight gain rate of 1% and 3% ...

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Abstract

The invention belongs to the technical field of development and research of feed additives, and specifically discloses an animal growth promoter containing Puccinia reedi, which contains Puccinia reedi with the nucleotide sequence shown in SEQ ID NO.1. Preparation method: 1. collection of teliospores of Puccinia reed; 2. drying; 3. crushing of reed leaves; 4. crushing of teliospores of Puccinia reed; In the invention, the teliospores of Puccinia reedi are broken and fed to animals in the form of feed additives, which can significantly promote the growth of animals in practice.

Description

technical field [0001] The invention belongs to the technical field of development and research of feed additives, and specifically discloses an animal growth promoter containing Puccinia reedi and a preparation method thereof. Background technique [0002] Animal growth promoter is a kind of animal feed additive, which plays an important role in animal husbandry production. For a long time, the research and application of feed additives has always been one of the priorities of animal nutritionists. After the discovery of antibiotics, antibiotics used as feed additives have played an important role in improving animal production capacity. However, after the 1940s, with the widespread application of antibiotics in the field of feeding, people gradually realized the disadvantages of extensive use of antibiotics, such as causing an imbalance in the normal flora of the gastrointestinal tract, reducing the level of immune function, producing drug resistance and drug residues and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18A23K10/30
CPCA23K10/18A23K10/30
Inventor 王登峰吴建勇李建军王文奇杨学云刘志强曹宗喜古努尔·吐尔逊
Owner VETERINARY INST XINJINAG ACADEMY OF ANIMAL SCI CLINIC MEDICAL SCI RES CENT XINJIANG ACADEMY OF ANIMAL HUSBANDRY SCI
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