Method and special device for predicting field apocynum rust
A technology of apocynum and rust fungus, which is applied in the field of apocynum rust prediction, and can solve the problems of leaf rot, false alarm, influence on infection and sporulation effect, etc.
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[0048] The present invention will be further described below in combination with specific embodiments.
[0049] Carrier production: Cultivate apocynum plants in a sterile environment and take their leaves as capture carriers.
[0050]a. Preparation of culture medium: choose commercially available vermiculite with coarser particles and good air permeability. Generally, it is better to choose the vermiculite with a particle diameter of 1-3mm. into the seedling tray.
[0051] b. Pregermination: Select 80-100g of apocynum seeds collected in the same year, disinfect with 0.1-0.5% sodium hypochlorite solution for 3-5 minutes, filter with gauze and rinse with sterile water for 3-5 times. Then put the seeds into a triangular flask, tie the mouth of the flask with a sealing film, and put it in a water bath at 40°C for 12-15 hours to accelerate germination. When the seed buds appear white, pour out the water in the triangular flask.
[0052] c. Colonization: The germinated seeds are ...
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