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A kind of open method for quickly cultivating microalgae

An open, microalgae technology, applied in the biological field, can solve the problem of promoting the growth of algae and the accumulation of oil in microalgae cells, and achieve the effect of increasing the amount of oil harvested, simple harvesting, and efficient growth process

Active Publication Date: 2016-08-17
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above-mentioned prior art is to control the growth balance of algae in the water body through bacteria, not to promote the growth of algae and the accumulation of oil in microalgae cells

Method used

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  • A kind of open method for quickly cultivating microalgae

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Embodiment Construction

[0025] The method for open-type rapid culture of microalgae of the present invention specifically includes the following content: before the semi-mixed culture, the two kinds of microorganisms are separately cultured in seed liquid, and a stirring bioreactor is used for culturing the seed liquid of facultative anaerobic bacteria, and photobiological The reactor is used for autotrophic microalgae seed liquid cultivation, the stirred bioreactor includes facultative anaerobic bacteria and anaerobic bacteria fermentation medium, and the photobioreactor includes microalgae species and autotrophic SE medium. After the seed solution is qualified, the facultative anaerobic bacteria are transferred to the lower area of ​​the open photobioreactor with a membrane separation device inside through the inoculation pipeline, and the microalgae cells are transferred to the upper area of ​​the photobioreactor through the inoculation pipeline. And add the aseptically treated mixed medium nutrien...

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Abstract

The invention discloses an open-type rapid culture method of microalgae. The method includes the steps of: (1) cultivating a facultative anaerobic bacteria seed liquid; (2) cultivating an autotrophic microalgae seed liquid; and (3) inoculating the facultative anaerobic bacteria seed liquid and the autotrophic microalgae seed liquid into an open-type photobioreactor with a built-in membrane separation device, which divides the photobioreactor into an upper zone and a lower zone, to carry out semi-mixing culture, fermenting the anaerobic bacteria in the zone below the membrane separation device, and subjecting the autotrophic microalgae to photosynthesis growth in the zone above the membrane separation device, wherein the medium composition and culture conditions are the same in the two zones, and supplementing organic carbon source required by the growth and metabolism of the facultative anaerobic bacteria in the culture process. The method has the advantages of increasing the yield of autotrophic microalgae, the utilization rate of an inorganic carbon source (CO2), and oil content of the microorganisms, simplifying the culture device and improving efficiency of large scale culture of microalgae.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an open method for cultivating microalgae, in particular to an open semi-mixed culture of autotrophic oil-producing microalgae and facultative anaerobic bacteria to obtain high microalgae cell harvest, while improving A method for the lipid content of microalgal cells. Background technique [0002] At present, oil resources are increasingly scarce, the contradiction between supply and demand of oil products is becoming more and more prominent, and the environmental pollution caused by the corresponding oil refining industry is also becoming more prominent. It is inevitable to develop clean and low-carbon alternative resources. Diesel derived from renewable resources such as microbial oil has high energy density, low sulfur content, sufficient combustion, and good lubricity. It is also renewable, environmentally friendly, easy to biodegrade, safe for storage and transportation, and good...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12N1/20C12R1/89C12R1/07C12R1/145C12R1/225C12R1/22
Inventor 廖莎王领民金平师文静李晓姝吴丹张霖高大成乔凯
Owner CHINA PETROLEUM & CHEM CORP
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