A method for the production of oil by coupled culture of microorganisms

Abstract

The invention discloses a production method for lipid through coupled culture of microbes. The method comprises the following steps: (1) culturing autotrophic microalgae seed liquid and carrying out enlarged culture until the OD value of biomass is 5.0 to 10.0, wherein the pH value of microalgae seed liquid having undergone enlarged culture is 9 to 12; (2) culturing facultative anaerobic bacteria seed liquid and carrying out fed-batch fermentation; and (3) during the process of fed-batch fermentation of facultative anaerobic bacteria, controlling the pH value in the fermentation process of the facultative anaerobic bacteria to be in a range of 6 to 9 in an illuminated bioreactor by using the prepared microalgae seed liquid having undergone enlarged culture in step (1) as an alkali neutralizing agent for bacterial fermentation and carrying out coupled culture of the facultative anaerobic bacteria and autotrophic microalgae. The method provided by the invention has the advantages of increased harvest yield of the autotrophic microalgae, an improved utilization rate of an inorganic carbon source, increased content of microbial lipid, a simplified culture apparatus and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a method for coupling culture of anaerobic bacteria and autotrophic oil-producing microalgae to obtain high cell yield and simultaneously harvest microbial oil. Background technique [0002] With the increasing shortage of oil resources, rising oil prices, increasingly prominent contradictions between supply and demand of oil products, and more prominent environmental pollution problems, it is inevitable to develop renewable oil resources through multiple channels. Diesel from microbial oil has high energy density, low sulfur content, sufficient combustion, and good lubricity. It is also renewable, easy to biodegrade, safe in storage and transportation, and good in antiknock performance. It is an ideal substitute for petrochemical energy. Taste. [0003] Microalgae is one of the oil-containing microorganisms. Microalgae are rich in protein, polysaccharides, unsaturated fa...

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Problems solved by technology

[0006] In the current literature, the cultivation of facultative anaerobic bacteria to produce oil and autotrophic microalgae is carried out for CO 2 The process of recharge and oxygen desorption to produce oleaginous microalgae biomass to obtain oil and other aspects are involved, but they are all cultured separately, and in the process of autotrophic culture of microalgae, CO 2 The problem of supplementation and its low fixation efficiency has not been well resolved. In order to overcome the current CO 2 Unfavorable factors such as unsatisfactory replenishment and oxygen analysis effects, cumbersome process, and excessive investment

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