The invention belongs to the technical field of tobacco genetic engineering, and specifically relates to a tobacco transcription repressor protein OFP1 and a patent application for its application. Tobacco transcription repressor protein OFP1 consists of 87 amino acid residues, of which the 34‑83 amino acid conserved domain. This protein is related to the content of pigment substances in plant leaves. After reducing the expression of this protein, the content of pigment substances in leaves is significantly reduced. The pigment substances are: neoxanthin, violaxanthin, lutein, chlorophyll a / b ,β-carotene. Through preliminary research on the tobacco transcription repressor protein OFP1 gene, the present invention finds that it is highly correlated with the content of pigment substances in tobacco, and after it is silenced, the content of pigment substances in tobacco is significantly reduced. Utilizing this characteristic can provide new reference and reference for the cultivation of new varieties of tobacco plants.