116 results about "Anthocyanin synthesis" patented technology

An isolated or recombinant MYB polypeptide having activity as a transcription factor in the synthesis of proanthocyanidins in plants, and nucleic acid molecule encoding same, wherein the polypeptide activates in the plants (a) promoters of the leucoanthocyanidin (LAR) and anthocyanidid reductase (ANR) genes, and (b) promoters of at least two of the genes of the general flavonoid pathway. Use of the polypeptide and nucleic acid molecule in regulating the biosynthesis and accumulation of proanthocyanidins in plants, such as in modifying pasture quality of legumes, is also disclosed.

The invention discloses a method for establishing a tomato nuclear male sterile line with a green stem marker, which comprises the following steps: simultaneously editing a fertility gene SlMs1035 ina receptor tomato genome and a linked marker gene SlF3H thereof by using a CRISPR / Cas9 system so as to cause the two genes to lose functions, and the tomato material obtained with the SlMs1035 and theSlF3H simultaneously losing function through ridding the transgene structure by carrying out selfing or hybridization is the tomato nuclear male sterile line with the green stem mark; wherein the marker gene SlF3H is an anthocyanin synthesis gene. As the stem of the sterile plants is green at the seedling stage, the sterile plants can be selected before the plants blossom. The method for establishing tomato nuclear male sterile line with the green stem marker adopts a molecular design breeding method to obtain a tomato nuclear male sterile line with a non-transgenic seedling stage marker character, and can be applied to transforming a tomato hybrid female parent into a nuclear sterile line with a green stem marker for tomato seed production through hybrid.

The invention relates to a wheat new gene ThMYC4E for regulating anthocyanin synthesis and metabolism. The gene ThMYC4E has a nucleotide sequence shown by the 1st-1,707th site of SEQ ID No.1 in the sequence table. In the invention, the obtained gene can be used for cultivating transfection plants so as to regulate anthocyanin synthesis.

The invention discloses a lycium barbarum gene, and a coding protein, a recombinant vector and application thereof. The amino acid sequence of the lycium barbarum gene is disclosed as SEQ ID NO. 1 or SEQ ID NO. 2. The nucleotide sequence is disclosed as SEQ ID NO. 3 or SEQ ID NO. 4. The invention also discloses a recombinant vector, expression cassette, transgenic cell line, recombinant bacterium or host cell containing the gene. The invention also discloses application of the gene in regulating anthocyanin synthesis, and a method for regulating anthocyanin synthesis in plants. The black lycium barbarum LMH1 and red lycium barbarum Ningqi7 fruit transcriptome is selected for sequencing, and the major gene for controlling the black fruit characters of the black lycium barbarum is analyzed and predicted, thereby providing foundation for researching the lycium barbarum anthocyanin synthesis mechanism. The invention also provides a direction and target for improving the anthocyanin content by modifying plants by gene engineering means.

The invention discloses a cDNA sequence of two MYB transcription factors (FhPAP1L1 and FhPAP1L2) which are cloned from petals of freesia refracta for the first time and are used for positively regulating anthocyanin synthesis, and a coded amino sequence is obtained through conjecture. Through over-expression of FhPAP1L1 and FhPAP1L2 in arabidopsis thaliana and tobacco, coloring of plant tissues can be significantly enhanced, so that the function of the FhPAP1L1 and FhPAP1L2 of participating in the positive regulation for plant anthocyanin synthesis is determined.

The invention belongs to the field of molecular biology, particularly relates to the field of molecular genetics, and particularly relates to an InDel molecular marker closely linked with an eggplantanthocyanin synthesis gene as well as a primer and application of the InDel molecular marker. For developing the molecular marker for controlling eggplant anthocyanin synthesis epistatic genes, the molecular marker InDel87335789 closely linked with the eggplant anthocyanin synthesis mutant pw gene is developed from a small associated region for restraining the eggplant anthocyanin synthesis mutantpw gene, wherein the eggplant anthocyanin synthesis mutant pw gene is positioned on the chromosome 8 of an eggplant, and the genetic distance between the molecular marker and a target gene P is 0.65cM. The molecular marker is expected to be applied to molecular marker assisted breeding of the eggplant anthocyanin synthesis mutant pw gene; and the molecular marker and the amplification primer ofthe molecular marker can be simply, conveniently and quickly applied to eggplant fruit color breeding practice with high throughput, and lay a good foundation for cloning an eggplant mutant pw gene and researching functions of the eggplant mutant pw gene.