Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Wheat new gene ThMYC4E for regulating anthocyanin synthesis and metabolism

An anthocyanin and gene technology, applied in genetic engineering, plant genetic improvement, angiosperms/flowering plants, etc., can solve problems such as plant phenotype loss

Active Publication Date: 2017-03-22
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
View PDF2 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Blockage of any of these reaction steps results in loss of plant phenotype

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Wheat new gene ThMYC4E for regulating anthocyanin synthesis and metabolism
  • Wheat new gene ThMYC4E for regulating anthocyanin synthesis and metabolism
  • Wheat new gene ThMYC4E for regulating anthocyanin synthesis and metabolism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 A new wheat gene ThMYC4E that regulates anthocyanin synthesis and metabolism, the gene ThMYC4E has the nucleotide sequence shown in SEQ ID NO. 1 from position 1 to position 1707 in the sequence table. It was screened from wheat varieties through specific primers ThMYC4EspF and ThMYC4EspR.

[0032] The protein encoded by the new wheat gene ThMYC4E, which regulates anthocyanin synthesis and metabolism, has the amino acid sequence shown in SEQ ID NO. 2 from position 1 to position 568 in the sequence table.

[0033] 1. Isolation of ThMYC4E gene

[0034] 1. Extraction of DNA and total RNA:

[0035] DNA was isolated from 1 gram of 10-day-old seedlings (using Yan ZH, Wan YF, Liu KF, Zheng YL, Wang DW, (2002) Identification of a novel HMW glutenin subunit and comparison of itsamino acid sequence with those of homologous subunits. Chinese ScienceBulletin 47:220-225 method). Total RNA was extracted from the aleurone layer of ten blue seeds using Tiangen RNAprep plant purifica...

Embodiment 2

[0100] Example 2 The new wheat gene ThMYC4E, which regulates the synthesis and metabolism of anthocyanins, was used as a recombinant vector, which was used as a part of the host cell.

Embodiment 3

[0101] Example 3 ThMYC4E, a new wheat gene that regulates anthocyanin synthesis and metabolism, is used as a part of host cells.

[0102] The host cells in the foregoing Examples 2 to 3 refer to cells other than human or animal germ cells or embryonic stem cells.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a wheat new gene ThMYC4E for regulating anthocyanin synthesis and metabolism. The gene ThMYC4E has a nucleotide sequence shown by the 1st-1,707th site of SEQ ID No.1 in the sequence table. In the invention, the obtained gene can be used for cultivating transfection plants so as to regulate anthocyanin synthesis.

Description

Technical field [0001] The invention relates to the field of plant genetic engineering, in particular to a new wheat gene ThMYC4E that regulates anthocyanin synthesis and metabolism. Background technique [0002] The aleurone layer of blue-grained wheat contains four main types of anthocyanins (Abdel-Aal, ESM and P. Hucl, Composition and stability of anthocyanins in blue-grained wheat. Journal of Agricultural and Food Chemistry, 2003. 51(8 ): p. 2174-2180). Anthocyanin gives plant organs and tissues colorful, and has the effect of attracting insects to help pollinate (Joaquin-Cruz, E., et al., Anthocyanin and phenolic characterization, chemical composition and antioxidant activity of chagalapoli (Ardisia compressa K.) fruit: A tropical source of natural pigments. Food Research International, 2015. 70: p. 151- 157), it also has the function of helping plants form specific disease resistance, protecting plants against biotic and abiotic stress factors, and from UV damage ( Treutt...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00
CPCC07K14/415C12N15/8243
Inventor 刘宝龙李娜张怀刚魏乐张波陈文杰刘登才
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com