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Application of osdhs gene in regulation of rice cuticle wax synthesis

An epidermal wax and gene technology, applied in application, genetic engineering, recombinant DNA technology, etc., can solve problems such as less research on transcription factors

Active Publication Date: 2019-08-09
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, researchers have cloned many genes of the wax synthesis pathway. These genes can regulate the amount or composition of wax, change the wax structure of the epidermis, and thus regulate the response of plants to drought stress. However, the research on transcription factors that regulate wax synthesis less

Method used

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  • Application of osdhs gene in regulation of rice cuticle wax synthesis
  • Application of osdhs gene in regulation of rice cuticle wax synthesis
  • Application of osdhs gene in regulation of rice cuticle wax synthesis

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specific Embodiment approach 1

[0042] Embodiment 1: The nucleotide sequence of the rice RING finger family E3 ubiquitin ligase OsDHS gene of this embodiment is shown in SEQ ID NO: 1 in the sequence listing.

specific Embodiment approach 2

[0043] Embodiment 2: The amino acid sequence of the encoded protein of the OsDHS gene in this embodiment is shown in SEQ ID NO: 2.

specific Embodiment approach 3

[0044] Embodiment 3: The application of the OsDHS gene in this embodiment in regulating the response of plants to drought stress.

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Abstract

The invention relates to and aims at providing a rice RING finge family E3 ubiquitin ligase OsDHS gene as well as an encoding protein and application thereof. The nucleotide sequence of the OsDHS gene is represented by SEQ ID NO:1 in a sequence table. The amino acid sequence of the encoding protein is represented by SEQ ID NO:2. By carrying out overexpression or knockout on the OsDHS gene in rice, a series of experiments prove that OsDHS gene overexpressed transgenic rice is super-sensitive to drought, and epicuticular wax is seriously lacked; otherwise, a dhs mutant presents that the drought resistance is enhanced. According to the OsDHS gene, by adjusting and controlling the synthesis of the epicuticular wax of the rice, the response of plants to drought stress is adjusted and controlled.

Description

technical field [0001] The present invention relates to an OsDHS gene, its encoded protein and its application. Background technique [0002] Rice is the main food crop for more than half of the world's population. With the development of industrialization and the increasingly serious water pollution, drought stress has become the main factor restricting rice yield. Fortunately, rice has developed a variety of mechanisms to resist drought stress. The waxy structure of the epidermis provides an important barrier to prevent water from evaporating through non-stomatal pathways. Increasing the wax content can significantly improve the drought resistance of rice. In recent years, researchers have cloned many wax synthesis pathway genes, which can regulate wax quantity or composition, change epidermal wax structure, and thus regulate plant response to drought stress. However, studies on transcription factors that regulate wax synthesis less. SUMMARY OF THE INVENTION [0003] ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/52C12N9/00C12N15/82A01H5/00A01H6/46
CPCC12N9/93C12N15/8273C12Y603/02019
Inventor 卜庆云王臻昱李秀峰田晓杰
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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