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Potato tuber anthocyanin synthesis transcription inhibitor StMYB44-1 and application thereof

A technology of anthocyanins and potato cubes, applied in the field of genes, can solve problems such as limited understanding of mechanisms

Pending Publication Date: 2019-07-09
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the understanding of the mechanism of MYB transcription factors regulating anthocyanin synthesis is still very limited, especially the inhibitory transcription factors of anthocyanin synthesis in potato tubers have not yet been discovered.

Method used

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  • Potato tuber anthocyanin synthesis transcription inhibitor StMYB44-1 and application thereof
  • Potato tuber anthocyanin synthesis transcription inhibitor StMYB44-1 and application thereof
  • Potato tuber anthocyanin synthesis transcription inhibitor StMYB44-1 and application thereof

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Embodiment

[0020] Example: The preparation method of the transcriptional repressor StMYB44-1 for anthocyanin synthesis in potato tubers is as follows: 1. Materials and methods

[0021] 1.1 Plant material and growth conditions

[0022] The tetraploid purple potato cultivar "Black Beauty" (HM, purple skin and purple pulp) was planted in 20 cm diameter flower pots and cultured in a light incubator at 21 / 17°C (day / night) with a light intensity of 200 μmol m -2 ·s -1 16-hour and 8-hour dark incubations. After the plants grew for one month, the plants were transferred to a light incubator at 26 / 22°C (day / night), and the control was still cultured in a light incubator at 21 / 17°C. After the tubers mature, take six fresh tubers for each treatment, carefully remove the skin with a scalpel, and cut the potato flesh into small pieces, immediately freeze the skin and flesh in liquid nitrogen and place them in a -80°C refrigerator middle.

[0023] 1.2 Reagents

[0024] Escherichia coli DH5α com...

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Abstract

The invention discloses a potato tuber anthocyanin synthesis transcription inhibitor StMYB44-1 and application thereof, wherein a gene sequence of the StMYB44-1 is shown as SEQ ID NO.1. A preparationmethod of the potato tuber anthocyanin synthesis transcription inhibitor StMYB44-1 comprises the following steps of: selecting plant materials; selecting reagents; extracting RNA and qPCR; constructing an expression vector; and cloning the StMYB44-1 gene sequence according to a designed primer of a potato database, and obtaining a complete coding sequence of the StMYB44-1 from potato meat of a black beauty potato by using cDNA as a template through PCR amplification. The invention discloses the transcription factor StMYB44-1 for inhibiting the potato tuber anthocyanin synthesis at high temperature; and the potato tuber anthocyanin synthesis transcription inhibitor StMYB44-1 has important significance for clarifying a regulation and control mechanism of the potato tuber anthocyanin under the influence of temperature and improving the content of the potato anthocyanin on the basis of the regulation and control mechanism.

Description

technical field [0001] The invention relates to the field of gene technology, in particular to the potato tuber anthocyanin synthesis transcriptional inhibitor StMYB44-1 and its application. Background technique [0002] Anthocyanins are flavonoids that determine the color of plants and play an important role in plant growth and human health. Anthocyanins are flavonoids produced in the process of plant secondary metabolism. They are the most important and widely distributed type of colored substances in plants, which endow plants with various colors. They not only make plants produce rich colors, but also play an important role in insect pollination, auxin transport, protection of leaves from ultraviolet rays, and inhibition of pests and diseases. As a natural pigment, anthocyanins can be used in food, cosmetics and other industries. At the same time, it has been proven to have anti-oxidation, anti-virus, delay the aging of body tissues, inhibit inflammation and allergies, ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/10
CPCC07K14/415C12N15/1096C12Q2531/113Y02A50/30
Inventor 刘玉汇李元铭王丽张俊莲刘震
Owner GANSU AGRI UNIV
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