44 results about "Anthocyanin biosynthesis" patented technology

The invention discloses a method for improving cotton traits, which is to inhibit or knock out the cotton anthocyanin methylation modification gene GhOMT1. The change of the cotton character of the present invention, by regulating the expression of the anthocyanin modification gene in the anthocyanin metabolic pathway, leads to the inability to synthesize the specific anthocyanin or anthocyanin that the cell needs, and changes the composition and content of the anthocyanin monomer , the feedback activates anthocyanin biosynthesis, resulting in the accumulation of a large amount of anthocyanins or intermediates, making the plant appear purple, and has anti-aging and anti-freezing properties.

The invention provides a transcription factor TaMYB3 of a MYB class, which is separated from the plateau 115 of a purple kernel wheat variety and is used for regulating the synthetization and the metabolization of anthocyanin. TaMYB3 is positioned between 0.62 and 0.95 of a long arm physical map of wheat 4B chromosome. The subcellular fraction of a protein product is positioned on a cell nucleus.Shown by a derived amino acid sequence, the TaMYB3 codes a MYB class transcription factor regulating the synthetization and the metabolization of anthocyanin. The expression quantity of the TaMYB3 inthe plateau 115 of a purple kernel wheat variety increases under the induction of light. The TaMYB3 can promote skin cells of the plateau 115 kernels after dark treatment to synthetize anthocyanin under the existence of transcription factors ZmR of corns bHLH during transient expression. Single TaMYB3 genes and single ZmR genes can not induct the synthetization of anthocyani, which shows that theTaMYB3 genes have the transcriptional activity of regulating the synthetization and the metabolization of anthocyanin. barly strip mosaic virus (BSMV) mediates the expression quantity decrease of theTaMYB3 in the plateau 115 kernels, and reduces the quantity of anthocyanin in kernels. This shows that the TaMYB3 participates in the biosynthesis of anthocyanin in the plateau 115 kernels.

The invention relates to the field of molecular biology, and discloses a bHLH transcription factor for promoting anthocyanin synthesis in lotus and application thereof in lotus flower color breeding.The CDS sequence of the bHLH transcription factor NnTT8 for promoting anthocyanin biosynthesis in the lotus is as shown in SEQ ID No. 1. The bHLH transcription factor NnTT8 for regulating anthocyaninbiosynthesis in lotus is researched for the first time, functional verification is performed on the bHLH transcription factor NnTT8 through an arabidopsis thaliana tt8 mutant recovery experiment, andmolecular breeding of lotus flowers is promoted.

The invention discloses a method for cultivating colored cotton. The method is one of the following: (1) using cotton purple mutant HS2 as a parent to cross with different varieties of cotton to obtain colored cotton; (2) knocking out and editing , interfere or overexpress key enzyme genes in cotton anthocyanin biosynthesis and metabolism pathway to obtain colored cotton; the key enzyme genes include PAL, C4H, 4CL-8, CHS, CHI, F3H, F3'H, F3'5 'H, DFR, LAR, LDOX, ANR, OMT or GST.

The invention provides a chrysanthemum bHLH transcription factor (CmbHLH2) involved in anthocyanin biosynthesis and regulation. A nucleotide sequence of the chrysanthemum bHLH transcription factor is shown as SEQ: NO.1. When the CmbHLH2 and a CmMYB6 coordinate to perform instantaneous over-expression in tobacco leaves, anthocyanin accumulation can be strongly induced to change the original green color of the leaves into a red color. Therefore, by means of transgenosis, over-expression of the CmbHLH2 in a plant is achieved or expression of the CmbHLH2 in the chrysanthemum plant is inhibited so that anthocyanin synthesis enhanced and inhibited transgenic plants can be obtained respectively, and the colors and luster of the transgenic plants can change with anthocyanin synthesis change.

The invention discloses a pyrus transcription factor PyERF3 as well as a recombinant expression vector and application thereof. A transcription factor PyERF3 gene which is separated from 'Starkrimson'pyrus and has an effect of promoting pyrus peel anthocyanin biosynthesis has a nucleotide sequence shown as SEQ ID No.1, and a coded amino acid sequence is shown as SEQ ID No.2 in a sequence table. The transcription factor is subjected to instantaneous conversion in tobacco, strawberry and pear fruits by virtue of an agrobacterium mediated genetic transformation method. Biological function verification proves that the PyERF3 gene cloned in the invention and other transcription factors PyMYB114 and PybHLH3 form transcriptional control complex so as to promote the biosynthesis of the pyrus peelanthocyanin. Discovery of the gene PyERF3 provides novel gene resources for promoting molecular breeding of pyrus peel anthocyanin synthesis and provides novel genetic resources for implementing green agriculture. Development and utilization of the genetic resources contribute to reduction of the agricultural cost and realization of environment friendliness.

The invention discloses a plant expression vector pC2300-pOT2-At-pri-miR828 based on an Arabidopsis thaliana (At)-pri-miR828 gene and construction and application thereof. The plant expression vector pC2300-pOT2-At-pri-miR828 contains the At-pri-miR828 gene and a pOT2 cloning vector CaMV35s promoter, and has a nucleotide sequence shown in SEQ ID NO. 5. After plants are subjected to transfection by the plant expression vector disclosed by the invention, plants with high expression of the At-pri-miR828 gene can be obtained, and the biosynthesis of anthocyanin in the plants is regulated and controlled.