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Litchi anthocyanin biosynthesis negative regulation gene and application thereof

A technology of biosynthesis and anthocyanins, which is applied in the fields of plant gene improvement, application, genetic engineering, etc., can solve the problems of negative regulatory factors that have not been reported yet, and achieve the effect of wide application prospects

Active Publication Date: 2018-12-11
SOUTH CHINA AGRI UNIV
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

However, there are still many key issues to be revealed in terms of its molecular regulation mechanism, such as the study of its negative regulators has not yet been reported

Method used

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  • Litchi anthocyanin biosynthesis negative regulation gene and application thereof
  • Litchi anthocyanin biosynthesis negative regulation gene and application thereof
  • Litchi anthocyanin biosynthesis negative regulation gene and application thereof

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Embodiment Construction

[0028] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the associated drawings. Preferred embodiments of the invention are shown in the accompanying drawings. However, the present invention can be embodied in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0029] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention.

[0030] 1. Screening and cloning of genes negatively regulated in litchi anthocyanin ...

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Abstract

The invention relates to a litchi anthocyanin biosynthesis negative regulation gene and application thereof. According to the invention, a litchi anthocyanin biosynthesis negative regulation gene is cloned (named LcMYBC2) for the first time, which is a MYB gene with a length of 708 bp and a number of encoded amino acids of 235. Through the plant transformation technology such as tobacco, the largeamount of expression of the negative regulation gene can inhibit the biosynthesis of tobacco anthocyanins and change the color of flowers. Therefore, the gene has broad application prospects in genetic engineering such as litchi coloring change and horticultural crop color gene in production.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a negative regulation gene for litchi anthocyanin biosynthesis and its application. Background technique [0002] Litchi (Litchi chinensis Sonn.) is an important south subtropical economic fruit tree in my country. It has very rich germplasm resources and has high edible value. Fruit coloring is one of the outstanding problems affecting the quality of litchi fruit, and poor coloring is a common genetic phenomenon in the development of litchi fruit. The coloring of litchi is good, and the commercial value of fruit quality is high, so fruit coloring is a very important index to evaluate the quality of litchi. The currently cultivated high-quality litchi varieties such as 'Concubine Xiao' in Guangdong Province have the problem of poor coloring. The formation and regulation of fruit color has always been a hot issue in the field of pomology. [0003] Prior to this, the i...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/02A01H6/82
CPCC07K14/415C12N15/825
Inventor 赵杰堂陈霖欢胡冰秦永华胡桂兵
Owner SOUTH CHINA AGRI UNIV
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