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Rice anthocyanin biosynthesis regulation gene OsTTG1 and application thereof

A technology for regulating genes and biosynthesis, applied in the direction of plant gene improvement, application, genetic engineering, etc., can solve problems such as limitations, achieve the effect of increasing expression levels and reducing anthocyanin content

Pending Publication Date: 2021-08-17
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the key WD40 gene for rice anthocyanin synthesis has not yet been identified, and no relevant patents have been published, which limits the use of genetic engineering technology to synthesize anthocyanins in rice

Method used

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  • Rice anthocyanin biosynthesis regulation gene OsTTG1 and application thereof
  • Rice anthocyanin biosynthesis regulation gene OsTTG1 and application thereof
  • Rice anthocyanin biosynthesis regulation gene OsTTG1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0036] (1) Using a single-gene segregation population to fine-map the anthocyanin synthesis gene OsTTG1:

[0037]Referring to the authorized patent "Method for constructing single-gene segregation populations of plant polygenes controlling traits" (ZL201911139749.3), the segregation population of OsTTG1 was constructed. Using molecular markers, 7 individual plants (heterozygous for OsTTG1 locus, homozygous for the other 9 loci and the same genotype as Donglan Momi) were screened out from the BC3F2 population of "Huanghuazhan × Donglan Momi", and a single gene was constructed. Separate groups. The seed coat color of 572 plants was identified, among which 433 were black and 139 were white, which met the segregation ratio of 3:1 (χ2=0.15figure 1 ).

[0038] (2) Functional annotation of OsTTG1 candidate genes:

[0039] Using the Nipponbare Genome Function Annotation Website MSU7 (http: / / rice.plantbiology.msu.edu / index.shtml) to annotate candidate genomic regions, 7 predicted gen...

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Abstract

The invention provides a rice anthocyanin biosynthesis regulation gene OsTTG1 and an application thereof, and the rice anthocyanin biosynthesis regulation gene OsTTG1 comprises a rice anthocyanin biosynthesis regulation gene OsTTG1, and the nucleotide sequence of the rice anthocyanin biosynthesis regulation gene OsTTG1 is shown as SEQ ID NO: 1. The invention relates to a biological material containing a gene OsTTG1. The invention also discloses the application of the gene OsTTG1 or the biological material in regulating and controlling the anthocyanin content of the plant and a method for reducing or increasing the anthocyanin content of the plant. The experiments prove that the content of anthocyanin in plant stems can be reduced by knocking out the OsTTG1, the content of the anthocyanin in the plants can be increased by increasing illumination or reducing temperature to increase the expression level of the OsTTG1, and a foundation is laid for researching postgraduate growth of rice rich in anthocyanin.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a rice anthocyanin biosynthesis regulation gene OsTTG1 and its application. Background technique [0002] Anthocyanins are a class of biologically active flavonoid compounds that accumulate in different tissues and organs of plants and have different biological functions, such as attracting pollinators, protecting plants against ultraviolet rays, and pests and diseases. In addition, anthocyanins, as phytonutrients, have strong antioxidant and anti-mutation functions, and play an important role in human health. [0003] Anthocyanin biosynthesis is mainly controlled by two types of genes, namely structural genes and regulatory genes. Structural genes encode enzymes directly involved in anthocyanin synthesis, which include two important gene groups: the upstream gene group (CHS, CHI, F3H, F3'H) and the downstream gene group (DFR, LAR, ANS, LDOX, ANR, GT). Upstream genes u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C12N15/11C12Q1/6895A01H5/00A01H6/46
CPCC07K14/415C12N15/825C12Q1/6895C12Q2600/13
Inventor 杨行海李丹婷邓国富曾宇张宗琼农保选夏秀忠吴艳艳荘洁
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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