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Composition and method for biosynthesis of anthocyanins

A biosynthesis, anthocyanin technology, applied in the field of plant genetic engineering, can solve problems such as lack of controllability

Pending Publication Date: 2021-12-31
HANGZHOU ADVANCED BIOSCIENCE CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the prior art, there is still a lack of anthocyanin biosynthesis methods with strong controllability, simple operation and high purity.

Method used

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  • Composition and method for biosynthesis of anthocyanins
  • Composition and method for biosynthesis of anthocyanins
  • Composition and method for biosynthesis of anthocyanins

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Experimental program
Comparison scheme
Effect test

Embodiment

[0048] 1. Experimental materials and reagents

[0049] 1.1 Test material

[0050] pCambia1302 vector, pUC19 vector: from Zhejiang University Agrobiology and Environmental Analysis and Testing Center Laboratory, as a commercial vector.

[0051] pGP1.1 vector: the pCambia1302 vector was obtained after modification of a few restriction enzyme sites;

[0052] pGP1.2 vector: the expression cassette of the target gene promoter and termination signal is added to the pUC19 vector;

[0053] The rice callus is derived from: Japonica rice Nipponbare induction (gifted by Jiangsu Academy of Agricultural Sciences);

[0054] The Agrobacterium used for gene transformation is: Agrobacterium tumefaciens EHA105;

[0055] Dihydroquercetin (DHQ): purchased from Nanjing Langze Biotechnology Co., Ltd., 99%.

[0056] Incubator model: intelligent artificial climate box DRX-450B (Ningbo Haishu Saifu Experimental Instrument Factory)

[0057] Shaker Model: DHZ-DA (Jiangsu Taicang Experimental Equipm...

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Abstract

The invention discloses a composition and method for biosynthesis of anthocyanins. According to the composition and the method, enzyme genes DFR, LDOX and UFGT in a plant anthocyanin biosynthesis pathway are utilized to construct a vector for the biosynthesis of the anthocyanins and a host cell containing the vector, the vector and the host cell are applied to the biosynthesis of the anthocyanins, and a different anthocyanin biosynthesis method is provided for the prior art. Further, through addition of different substrates for the DFR, technicians in the field can obtain the anthocyanins with different colors.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to a composition and method for utilizing plant callus cells to perform anthocyanin biosynthesis. Background technique [0002] Anthocyanins are a class of secondary metabolites that widely exist in plants and belong to flavonoids. Anthocyanins endow plants with colorful colors, and in the process of plant development, they have important physiological functions such as protecting plants from ultraviolet radiation and resisting pathogenic bacteria. At the same time, anthocyanins have important curative effects in treating heart disease and cancer, scavenging free radicals, anti-oxidation, and anti-aging. Because of its good color performance, health and safety, it has been widely used in various industries such as food, cosmetics and medicine, and it is also a hot field of plant research. [0003] At present, there are many methods for the preparation of anthocya...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/84C12N15/53C12N15/54C12N15/65C12N5/10C12N1/21
CPCC12N15/825C12N15/8209C12N15/821C12N15/8205C12N9/0006C12N9/0071C12N9/1051C12Y101/01219C12Y114/11019C12Y204/01009
Inventor 王益行吕晓荣宋淼泉凌建群
Owner HANGZHOU ADVANCED BIOSCIENCE CO LTD
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