Method for preparing liquid mycelium fermentation culture medium formula of selenium-rich cordyceps militaris

Abstract

The invention relates to a method for preparing a liquid mycelium fermentation culture medium formula of selenium-rich cordyceps militaris. The method takes natural colored silkworm cocoon sericin protein and a selenium-rich yeast as a main nitrogen source, takes mixed coarse food grains including rice, Chinese yam, potatoes, wheat, millet and corn as a main carbon source, takes organic selenium in the selenium-rich yeast as a selenium source, takes lentinan and adenosine as motivating factors, and takes other trace factors as auxiliary factors to manufacture the liquid mycelium fermentation culture medium formula of selenium-rich cordyceps militaris. The method effectively recycles abandoned husks and cocoon shells, the cost of the nitrogen source is greatly reduced, the nitrogen source in a culture medium has good bacterial resistance and antioxidant activity, and the fermentation process is not easily contaminated by bacteria. The selenium-rich yeast in the culture medium plays a role of supplementing the nitrogen source and is also a selenium source. Through the addition of the lentinan and the adenosine, the metabolism and growth of selenium-rich cordyceps militaris mycelium can be significantly improved, and the output of active materials such as cordyceps polysaccharide, cordycepic acid, cordycepin and the like is greatly increased.

Description

technical field [0001] The invention relates to a preparation method of a novel medium formula for liquid fermentation of Cordyceps militaris, in particular to a preparation method of a liquid mycelium fermentation medium formula of selenium-enriched Cordyceps militaris, belonging to the technical field of biopharmaceutical production. Background technique [0002] Cordyceps militaris ( C o r d c e p s mi l i t a r i s ) of the genus Ascomycota ( A s c o my c o t i n a ) Ergotaceae ( C l a v i e i p i t a e e u e ) Cordyceps ( C o r d y c e p s ), is a medicinal fungus belonging to the same genus as Cordyceps sinensis, and the number of natural resources distributed in the world is very small. Cordycepin (3'-deoxyadenosine), cordycepic acid, and polysaccharides in Cordyceps militaris have significant effects such as antibacterial, anti-inflammatory, anti-aging, anti-tumor, immune regulation, and regulation of blood lipid and blood sugar, and are important active ingre...

AI Technical Summary

Problems solved by technology

In the traditional cultivation process of selenium-enriched Cordyceps militaris mycelium, inorganic selenium sodium selenite is generally added to the culture medium, which is converted into organic selenium after being absorbed by the mycelium. This method is not only difficult for the mycelium to absorb , Sodium selenite is also toxic to mycelia and has great defects