The invention discloses an
industrial fermentation method of
riboflavin, which comprises the following steps of: filling a seed culture medium into a 10m<3> seed tank, and performing oxic cultivation; and filling a
fermentation medium into a 200m<3>
fermentation tank for
fermentation, mechanically stirring, adding sterilization
starch glucose liquid, stopping fermenting till that the glucose is completely consumed or the dissolved
oxygen is raised to be 60%, and extracting the
riboflavin from the fermentation liquid. After the
industrial fermentation method is used, the conventional
riboflavin material-supplementing fermentation technology is changed, the low-concentration
raw material cultivating
bacteria is adopted, the material is quantificationally supplemented according to the changeof the dissolved
oxygen of the fermentation solution, the excessive multiplication of the
thallus can be effectively controlled, the deviation of the
optimum control temperature caused by the excessive supersession can be avoided, the defects of the insufficient ventilation and
oxygen supply can be remitted, and the
thallus vigor can be kept, so that the indusial fermentation unit of the riboflavin is improved by more than 18g / L, and the yield is increased by 20% compared with the common fermentation.