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Silk protein powder and preparation method thereof

The technology of silk protein powder and silk fibroin is applied in the field of silk protein powder and its preparation, which can solve the problems of incomplete removal of odor substances and pigments, small molecular weight of silk amino acids, poor water solubility of silk fibroin powder, etc. Moderate molecular weight, short time effect

Inactive Publication Date: 2013-09-11
湖州新天丝生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]Our country is very rich in silk resources. For decades, many intellectuals have been engaged in the development and research of silk resources, and research theories and articles have been published from time to time. Silk protein products are mainly silk fibroin powder, sericin powder and silk amino acid. Due to poor water solubility of silk fibroin powder, high impurities in sericin powder and too small molecular weight of silk amino acid, the removal of odorous substances and pigments is not complete, and the biological activity is seriously damaged. Moreover, these products have high heavy metals and ash content, which greatly hinders their application in the fields of biology, medicine, food, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method of silk protein powder, comprising the steps of:

[0030] 1. Degumming of silk:

[0031] (1) Weigh 100 kilograms of silk, soak the silk in tap water for 13 hours, wash it with pure water for 5 times, and dry it with a centrifuge;

[0032] (2) According to the bath ratio (weight ratio) of silk: water = 1:6, add 100kg silk and 600kg pure water into the high-pressure degumming kettle, heat the high-temperature steam to boiling, keep the steam pressure of 0.15 MPa, and release the sericin solution for 1 hour 522kg. The measured solid content is 4.99%, and the dry solid is 26.05kg. The silk fibroin that takes out is dried, weighs dry solid 71kg.

[0033] 2. Enzymatic hydrolysis of silk protein:

[0034] Carry out enzymatic hydrolysis to sericin solution and silk fibroin respectively:

[0035] Put 420kg of sericin solution into the enzymatic hydrolysis tank and heat it to 45°C, adjust the pH value to 7.2, add Flavourzyme enzyme (7.8g of enzyme) a...

Embodiment 2

[0046] A preparation method of silk protein powder, comprising the steps of:

[0047] 1. Degumming of silk:

[0048] (1) Weigh 100 kilograms of silk, soak the silk in tap water for 14 hours, wash it with pure water for 5 times, and dry it with a centrifuge;

[0049] (2) According to the bath ratio (weight ratio) of silk: water = 1:8, add 100kg silk and 800kg pure water into the high-pressure degumming kettle, heat the high-temperature steam to boiling, keep the steam pressure of 0.17 MPa, release the liquid for 0.6 hours, and take it out Sericin solution 518kg. The measured solid content was 4.94%, and 25.6 kg of dry solid was obtained. The silk fibroin that takes out is dried, weighs dry solid 73kg.

[0050] 2. Enzymatic hydrolysis of silk protein:

[0051] Carry out enzymatic hydrolysis to sericin solution and silk fibroin respectively:

[0052] Put 418kg of sericin solution into the enzymatic hydrolysis tank and heat it to 45°C, adjust the pH value to 7.2, add Flavou...

Embodiment 3

[0063] A preparation method of silk protein powder, comprising the steps of:

[0064] 1. Degumming of silk:

[0065] (1) Weigh 100 kilograms of silk, soak the silk in tap water for 15 hours, wash it with pure water for 5 times, and dry it with a centrifuge;

[0066] (2) According to the bath ratio (weight ratio) of silk: water = 1:9, add 100kg silk and 900kg pure water into the high-pressure degumming kettle, heat the high-temperature steam to boiling, keep the steam pressure of 0.19 MPa, release the liquid for 0.5 hours, and take it out Sericin solution 522kg. The measured solid content was 4.85%, and 25.3 kg of dry solid was obtained. The silk fibroin that takes out is dried, weighs dry solid 70kg.

[0067] 2. Enzymatic hydrolysis of silk protein:

[0068] Carry out enzymatic hydrolysis to sericin solution and silk fibroin respectively:

[0069] Put 422kg of sericin solution into the enzymatic hydrolysis tank and heat it to 45°C, adjust the pH value to 7.2, add Flav...

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PUM

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Abstract

The invention discloses silk protein powder and a preparation method thereof. The preparation method comprises the following steps of: (1) degumming silks; (2) performing enzymolysis on silk fibroins and sericin; (3) separating and purifying silk proteins; and (4) pre-concentrating and drying a silk protein liquid. The silk protein powder disclosed by the invention comprises sericin proteins and silk fibroin proteins, has the advantages of high purity, high biological activity, proper molecular weight, white color, zero foreign smell and the like and can be used for making biological and medical materials, health protection food and the like, and the aims of pure nature, high activity, high functionality, rich nutrition and the like are fulfilled.

Description

technical field [0001] The invention relates to a silk protein powder and a preparation method thereof. Background technique [0002] Silk protein includes silk fibroin and sericin, which are rich in 18 kinds of amino acids, of which glycine, alanine, and serine account for more than 70% of silk fibroin, and more than 73% of polar side group amino acids of sericin. It is a natural nutritional substance with outstanding functions, which is widely used in biology, medicine, food, cosmetics, electronic materials, leather and fabrics, etc. [0003] Our country is very rich in silk resources. For decades, many intellectuals have engaged in the development and research of silk resources, and published research theories and articles from time to time. But so far, the silk protein products produced in China are mainly silk fibroin powder, sericin powder and silk amino acids. , due to the poor water solubility of silk fibroin powder, high impurities in sericin powder and too small m...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C07K14/435
Inventor 徐国文
Owner 湖州新天丝生物技术有限公司
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