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Drying production technique by spraying dry oceanic rhodotorula

A technology of marine red yeast and its production method, applied in the field of spray-drying production of solid marine red yeast, can solve the problems of large-scale production of cold storage, long-term storage and long-distance transportation, low concentration of marine red yeast cells, and slow growth of marine red yeast and other issues, to achieve the effect of improving health status, improving shelf life, and facilitating transportation

Inactive Publication Date: 2003-05-07
安琪酵母(滨州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the disadvantages of slow growth of marine red yeast, long production cycle, low cell concentration of fermented marine red yeast, short shelf life of the final liquid product, need for refrigeration, etc., which are not conducive to large-scale production, long-term storage and long-distance transportation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

manufacture example 1

[0048] On the slant of the malt juice agar medium test tube with a medium of 8Be, inoculate Rhodotorula viscosus strains in the medium under sterile conditions, control the culture temperature at 30°C, and cultivate for 48 hours to grow pink circles. Formed bacterial colonies are appropriate. Then above-mentioned bacterial classification is connected in two 500ml Erlenmeyer flasks under aseptic condition, (150ml wort culture fluid is installed in the Erlenmeyer flasks) carries out expansion culture, controls culture temperature 30 ℃, shakes flask and cultivates 48 hours under 200rpm. Then the above-mentioned triangular flask strains were respectively inserted into two Karl Fischer tanks each equipped with 20 liters of 8Be wort sterile culture solution, and cultured with magnetic stirring at 30° C. for 48 hours. Finally, put the seeds of the above-mentioned cultured Caspian tank into the sterilized pure culture tank with 5 tons of medium for aeration and fermentation culture, t...

manufacture example 2

[0052] On the slant of the malt juice agar medium test tube whose medium is 10Be, inoculate Rhodotorula viscosus strains in the medium under sterile conditions, control the culture temperature at 26°C, and cultivate for 26 hours to grow pink circles. colonies with a diameter of 1 mm. Then above-mentioned bacterial classification is connected in two 500ml Erlenmeyer flasks under aseptic condition, (150ml wort culture liquid is installed in the Erlenmeyer flasks) carries out expansion culture, controls culture temperature 26 ℃, shakes flask and cultivates 36 hours under 100rpm. Then the above-mentioned triangular flask bacterial strains were respectively inserted into two Karl Fischer tanks each equipped with 20 liters of wort sterile culture solution of 8Be, and cultured with magnetic stirring at 26° C. for 36 hours. Finally, put the seeds of the above-mentioned cultured Caspian tank into the sterilized pure culture tank with 3 tons of medium for aeration and fermentation cultu...

manufacture example 3

[0056] On the slant of the malt juice agar medium test tube whose medium is 12Be, inoculate Rhodotorula viscosus strains in the medium under sterile conditions, control the culture temperature at 31°C, and cultivate for 72 hours to grow pink circles. shaped colonies with a diameter of 2 mm. Then above-mentioned bacterial classification is connected in two 500ml Erlenmeyer flasks under aseptic condition, (150ml wort culture liquid is installed in the Erlenmeyer flasks) carries out expansion culture, controls culture temperature 31 ℃, shakes flask and cultivates 45 hours under 250rpm. Then, the bacteria strains in the above-mentioned triangular flasks were respectively inserted into two Karl Fischer tanks each equipped with 20 liters of 8Be wort sterile culture solution, and cultured with magnetic stirring at 31° C. for 45 hours. Finally, put the seeds of the above-mentioned cultivated Caspian tank into the sterilized pure culture tank with 4 tons of medium for aeration and ferm...

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Abstract

A spray process for drying solid marine rhodotorule features use of continuous stream plus deep aeration culture and spray drying. Its resultant has high concentration of rhodotorula cells (10-100 billions / g) and high output. It can be used to raise prawn and marin crab for higher surviral rate, health level and raising efficiency.

Description

technical field [0001] The invention relates to a production method of marine red yeast, in particular to a spray drying production method of solid marine red yeast. Background technique [0002] As a biological bait, marine rhodotorula is currently widely used in seedling and adult culture of aquatic products such as shrimp, crab, and shellfish. Chinese patent discloses a kind of invention name and is called " fresh and alive unicellular marine red yeast ecological bait and production method thereof ", publication number is CN1146290A, and publication date is April 24, 1997. In the description, the invention discloses a method for culturing marine rhodotorula and producing liquid products. The method involves a method in which marine rhodotorula is isolated from nature, fed with liquid for one feeding, and processed into a liquid product after post-processing. The method has the disadvantages of slow growth of marine red yeast, long production...

Claims

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Application Information

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IPC IPC(8): A23K10/16A23L31/10C12M1/04C12N1/16F26B3/12
Inventor 姚娟谭斌
Owner 安琪酵母(滨州)有限公司
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