Orange biological preserving agent based on yeast, sodium bicarbonate and elicitor activity
A technology of biological preservative and sodium bicarbonate, which is applied in the fields of fruit and vegetable preservation, food preservation, and food science. The effect of food safety
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[0026] The preparation methods of 3 kinds of yeast suspensions are as follows respectively:
[0027] The NYDA culture medium used below is: beef extract 8g, yeast powder 5g, glucose 10g, agar 20 grams, water is settled to 1000ml, high pressure (1kg / cm ) 121 ℃ of sterilization 30 minutes.
[0028] The NYDB seed culture medium used is: 8 g of beef extract, 5 g of yeast powder, 10 g of glucose, the volume of water is adjusted to 1000 ml, and high pressure (1 kg / cm 2 ) is sterilized at 121° C. for 30 minutes.
[0029] Cryptococcus laurentis, Rhodotorula viscosus, and Rhodosporidium were respectively stored in NYDA medium at low temperature (4°C). The training program is as follows:
[0030] ①. Activation: First culture in NYDA medium at 28°C for 48 hours, then repeat subculture twice (each time in NYDA medium at 28°C for 48 hours); activate the above-mentioned repeated subculture twice. The yeasts obtained from the NYDB seed medium were connected to the NYDB seed medium with an ...
Embodiment 1
[0034] Embodiment 1, the citrus biological preservative based on yeast, sodium bicarbonate and elicitor activity:
[0035] First, separately activate and cultivate Cryptococcus laurentis, Rhodotorula viscosus and Rhodosporidium; adjust the concentrations of the three yeasts to 2×10 9 cells / ml, take 0.5 liter volume of Cryptococcus lorensis suspension, add 0.5 liter volume of Rhodotorula viscosus suspension, stir and mix for 3 minutes; then add 0.5 liter volume of Rhodosporidium suspension, after the completion Continue to stir and mix for 3 minutes, slowly add 1000 ml of SA solution with a concentration of 1000 mg / L, stir and mix for 5 minutes after the SA solution is added; then slowly add 100 ml of IBA solution with a concentration of 1000 mg / L, and stir for 5 minutes Slowly add 2500 milliliters of sodium bicarbonate solution with a concentration of 80 g / L, stir and mix for 5 minutes and add water to dilute to 10 liters for subsequent use after the solution has been added. ...
Embodiment 2
[0036] Embodiment 2, adjust the consumption of SA solution, sodium bicarbonate solution and IBA solution among the embodiment 1; Thereby make the every L citrus biological preservative of final gained contain respectively 1 * 10 11 Cryptococcus laurentis, 1×10 11 sticky red yeast, 1 x 10 11 Rhodosporidium yeast cells, 50mg / L SA, 200mg / L IBA, 25g / L sodium bicarbonate, and the rest are water.
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