Method of applying immobilized lipase to catalyze ester hydrolysis for kinetic resolution of 2-(4-methylphenyl) propionic acid enantiomers
A lipase-catalyzed ester and immobilized lipase technology, applied in the direction of immobilized enzymes, biochemical equipment and methods, fixed on/in organic carriers, etc., can solve the problems of low loading rate and mass transfer performance of immobilized enzymes Poor quality, short service life of polymer materials, etc., to achieve enhanced stability and operating range, easy separation, and good reusability
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Embodiment 1
[0014] Mix 0.01mmol racemic methyl 2-(4-methylphenyl)propionate and 50mg immobilized enzyme AYS@UIO-66-NH 2 Add it into a 10mL reaction tube, and add 2mL of phosphate buffer solution (pH=6.60, 0.1mmoL / L) as the reaction solution, and heat the reaction at 500rpm and 39°C for 24h; The ratio and the enantiomeric excess of the product were analyzed. The analysis results showed that the yield of (R)-2-(4-methylphenyl)propionic acid was 72.567%, and the enantiomeric excess was 93.202%.
Embodiment 2
[0016] 0.01 mmol of racemic 2-(4-methylphenyl) propionate methyl ester and 50 mg of immobilized enzyme AYS@UIO-66-NH 2 Put it into a 10mL reaction tube, and add 2mL of phosphate buffer solution (pH=6.60, 0.1mmoL / L) as the reaction solution, and catalyze the reaction at a rotation speed of 500rpm and a reaction temperature of 60°C for 24h. After the reaction, use high-performance liquid chromatography Yield and product enantiomeric excess were analyzed by instrument. The analysis results showed that the yield of (R)-2-(4-methylphenyl)propionic acid remained at 70.458%, and the enantiomeric excess was 91.789%.
Embodiment 3
[0018] 0.01 mmol of racemic 2-(4-methylphenyl) propionate methyl ester and 50 mg of immobilized enzyme AYS@UIO-66-NH 2 Put it into a 10mL reaction tube, and add 2mL of phosphate buffer solution (0.1mmoL / L), the pH value is 8.5, as the reaction solution, catalyze the reaction at a rotation speed of 500rpm and a reaction temperature of 39°C for 24h. Chromatography was used to analyze the yield and enantiomeric excess of the product. The analysis results showed that the yield of (R)-2-(4-methylphenyl)propionic acid remained at 70.023%, and the enantiomeric excess was 90.924%.
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