30 results about "Streptomyces globisporus" patented technology

The invention discloses a deodorant special for livestock and poultry farms and application thereof, wherein the deodorant is composed of the following strains: 106-108 lactobacillus acidophilus per milliliter, 107-109 lactobacillus reuteri per milliliter, 104-106 bacillus polymyxa per milliliter, 106-108 bacillus lentus per milliliter, 106-109 bacillus coagulans per milliliter, 104-106 enterococcus faecalis per milliliter, 107-109 enterococcus faecium per milliliter, 104-107 candida rugosa per milliliter, 104-107 candida valida per milliliter, 104-106 pseudomonas farinofermentans per milliliter, 106-108 rhodopseudomonas palustris per milliliter, 104-106 geotrichum suaveolens per milliliter, 106-108 aspergillus clavatus per milliliter, 106-108 rough spore aspergillus per milliliter and 106-109 streptomyces globisporus per milliliter. According to the invention, excrements in a poultry house and excrements for accumulative fermentation are treated through a spraying manner, so as to effectively reduce the release amount of malodorous gases, and provide a technical support for the effective treatment of malodor in the livestock and poultry farms and the environmental protection. Besides, the deodorant special for livestock and poultry farms can also be sprayed to the livestock and poultry body surfaces, to keep the livestock and poultry body surfaces clean and sanitary, and be conducive to the growth of livestock and poultry.

The invention provides a humectant used for dipping root in seedling transplantation, and a preparation method thereof. The humectant comprises, by weight, 15 to 26 parts of carboxymethyl cellulose, 5 to 12 parts of humic acid, 10 to 15 parts of a benzimidazole fungicide, 10 to 20 parts of potassium dihydrogen phosphate, 15 to 30 parts of a super absorbent resin, 100 to 200 parts of starch grafted sodium acrylate, 100 to 200 parts of sodium naphthalene-1-acetate, 450 to 900 parts of talcum powder, 300 to 600 parts of Streptomyces globisporus fermentation broth, and 200 to 500 parts of water. The humectant is capable of promoting healing of seedling root wounds, and rooting, and increasing survival rate in seedling transportation process.

The invention belongs to the technical field of agro-microbiology, and in particular relates to the isolation and identification of a biological control streptomyces globisporus strain and the application of the biological control streptomyces globisporus strain in prevention and control of the penicilliosis of citrus. In the invention, the streptomyces globisporus strain JK-1 capable of producing antifungal volatile substances is obtained, is streptomyces globisporus and is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO: M2010093. The invention also provides microbial agents prepared from the streptomyces globisporus strain JK-1. The volatile substances produced by the streptomyces globisporus strain JK-1 are utilized to prevent and control the penicilliosis of the citrus in preservation and freshness, which indicates that microorganisms and the agents thereof belong to novel biological control agents.

The invention discloses rooting liquid for cuttings of fruit trees and a preparation method of the rooting liquid. The rooting liquid comprises the following raw materials in parts by weight: 4-8 parts of an acetic acid complex, 8-12 parts of fermented liquid of streptomyces globisporus, 10-12 parts of a nutrient, 2-4 parts of boron trioxide, 4-8 parts of ammonium nitrate, 5-7 parts of humic acid,10-15 parts of a tobacco root extract, 4-6 parts of ascharite ore, 6-8 parts of zinc ore, 5-7 parts of Chinese medicinal herb juice, 5-7 parts of beta-glucan, 0.3-0.7 part of bamboo vinegar, 1-3 parts of ethanol and 15-25 parts of water. The rooting liquid provided by the invention can quickly heal wounded tissues, promotes growth of roots and survival of cuttings, can effectively protect woundsand prevent infection of germs, reduces water evaporation, stimulates tree cell division to form callus and rapidly grow new barks, and greatly improves a survival rate of the fruit trees.

The invention relates to a new streptomyces secretion expression plasmid and application thereof. The new streptomyces secretionexpression plasmid is particularly characterized by being cloned with a colon bacillus replication original region ori, a streptomyces conjugational-transfer essential region oriT, a penbritin resistance gene bla, an apramycin resistance gene aac (3) IV, a promoter ermE*p, a lidamycin prosthetic-group protein gene promoter cagAp, a lidamycin prosthetic-group protein gene mutation type signal peptide SPcagA (CTG) and multiple cloning sites by taking a minimum replicon of a pSGL1 plasmid naturally existing in streptomyces globisporus C-1027 as a framework. The new streptomyces secretory expression plasmid can be used for efficiently secreting and expressing various heterologous proteins in streptomyces.

The invention discloses a novel microbial organic fertilizer for preventing and treating perennial root dwarf disease of Saccharum officinarum. The novel microbial organic fertilizer is prepared through the following steps: mixing animal wastes and straw powder, inoculating well-composted mixed bacteria to the mixture, and carrying out compost fermentation, so as to obtain a well-composted material; carrying out amplification culture on bacillus thuringiensis so as to obtain a culture solution for pathogen inhibiting bacteria for perennial root dwarf disease of Saccharum officinarum; carrying out amplification culture on streptomyces globisporus and bacillus megaterium, and carrying out proportioning mixing, so as to obtain an effect promoting bacterium mixed solution; proportionally mixing bamboo charcoal, diatomite and zeolite powder, so as to obtain a soil amendment; and uniformly mixing the well-composted material, the culture solution for the pathogen inhibiting bacteria for the perennial root dwarf disease of the Saccharum officinarum, the effect promoting bacterium mixed solution and the soil amendment proportionally, and carrying out drying, crushing, sieving and granulating, thereby obtaining the microbial organic fertilizer. The microbial organic fertilizer provided by the invention is high in fertility, can be used for truly effectively inhibiting the incidence of the perennial root dwarf disease of the Saccharum officinarum and can also be used for amending soil, improving the activity of the soil, increasing the yield of the Saccharum officinarum and shortening the ripening period of the Saccharum officinarum, thereby having very high economic and social benefits.

The invention relates to a composition for inhibiting growth of candida albicans and a preparation method and application thereof. The composition is prepared from a first component and a second component according to a mass ratio of 10:(1 to 2), wherein the first component comprises a fermenting product which is fermented by streptomyces griseus and streptomyces globisporus; the second component is prepared from the following components in parts by weight: 15 to 20 parts of vitamin B2, 8 to 12 parts of polyhexamethylene biguanide, 3 to 5 parts of citric acid, 0.5 to 1 part of glycyrrhizin, and 1 to 2 parts of sodium benzoate. The composition for inhibiting the growth of candida albicans has the advantages that by utilizing the streptomyces griseus and the streptomyces globisporus to ferment together, the obtained fermenting product is used as one of components; the prepared composition has the function of inhibiting the growth of candida albicans, and the inhibiting effect is obvious; the preparation method is simple, the production cost is low, and the quality is stable.

The invention discloses a disinfector used for shrimp eggs. The disinfector contains the following components in parts by weight: 3-8 parts of streptomyces globisporus, 4-7 parts of crab shell extracts, 5-10 parts of limonene, 1-3 parts of beta-phellandrene, 2-5 parts of caryophyllene and 3-6 parts of citric acid. The disinfector disclosed by the invention has the beneficial effects that the toxicity to pathogene is strong, and a small amount of disinfector can play a good disinfection role; the shrimp eggs have better tolerance to the disinfector, the usage range of the disinfector is enlarged, and the disinfection time of the disinfector is prolonged; a preparation method of the disinfector is simple, the used time is short, the quality stability of the disinfector is good, the cost is low and the application effect is good, also the preparation method has good economical benefits, and is an environment-friendly process.

The invention discloses a biological agent for preventing and treating tobacco red spot disease. The production strain of the biological preparation is classified as Streptomyces globisporus AM6, and the preservation number is CGMCC No.1701. The liquid and solid culture medium formula of described bacterial strain is as follows: 1. test tube slant culture medium formula is: fresh potato 200g, glucose 20g, agar 17-20g, distilled water 1000ml, pH is natural; 2. liquid fermentation medium formula is: soybean flour 10.0g , 20.0g sucrose, 2g dipotassium hydrogen phosphate, 0.02g ferrous sulfate, 0.5g calcium carbonate, 0.05g magnesium sulfate, 1000ml tap water, natural pH. The invention is prepared as a biological preparation through the test tube culture of bacterial strains and shaker fermentation culture. The test results show that the biological agent provided by the invention has a good control effect on tobacco red spot disease.

The invention discloses a deodorant special for livestock and poultry farms and application thereof, wherein the deodorant is composed of the following strains: 106-108 lactobacillus acidophilus per milliliter, 107-109 lactobacillus reuteri per milliliter, 104-106 bacillus polymyxa per milliliter, 106-108 bacillus lentus per milliliter, 106-109 bacillus coagulans per milliliter, 104-106 enterococcus faecalis per milliliter, 107-109 enterococcus faecium per milliliter, 104-107 candida rugosa per milliliter, 104-107 candida valida per milliliter, 104-106 pseudomonas farinofermentans per milliliter, 106-108 rhodopseudomonas palustris per milliliter, 104-106 geotrichum suaveolens per milliliter, 106-108 aspergillus clavatus per milliliter, 106-108 rough spore aspergillus per milliliter and 106-109 streptomyces globisporus per milliliter. According to the invention, excrements in a poultry house and excrements for accumulative fermentation are treated through a spraying manner, so as to effectively reduce the release amount of malodorous gases, and provide a technical support for the effective treatment of malodor in the livestock and poultry farms and the environmental protection. Besides, the deodorant special for livestock and poultry farms can also be sprayed to the livestock and poultry body surfaces, to keep the livestock and poultry body surfaces clean and sanitary, and be conducive to the growth of livestock and poultry.

The invention discloses an incubation breeding method of Cheraxquadricarinatus. The method comprises following steps of parent shrimp rearing, egg removal, disinfection, implantation and hatching. An adopted disinfectant comprises the following components such as 3-8 parts by weight of streptomycesglobisporus fermentation liquid, 4-7 parts by weight of crab shell extractives, 5-10 parts by weight of limonene, 1-3 parts by weight of Beta-phellandrene, 2-5 parts by weight of caryophyllene, and 3-6 parts by weight of citric acid. The incubation breeding method of Cheraxquadricarinatus has the following beneficial effects: the disinfectant employed by the method is highly toxic for pathogens; a little disinfectant can exert good effect; shrimp eggs are highly resistant to the disinfectant; while pathogens are killed, the hatching rate is not affected; the survival rate of raised larval shrimps is high; larval shrimps are good in quality; the breeding steps are easy; operation is conveniently; and the method is highly practical and generates good economic benefit.

The invention discloses a method for disinfecting shrimp eggs without affecting hatchability, which comprises the steps of loosening eggs, preparing a disinfectant, preparing disinfectant liquid and disinfecting, wherein the disinfectant is prepared from the following components in parts by weight: 3 to 8 parts of streptomyces globisporus fermentation liquor, 4 to 7 parts of crab shell extract, 5 to 10 parts of limonene, 1 to 3 parts of beta-phellandrene, 2 to 5 parts of caryophyllene and 3 to 6 parts of citric acid. The method has the following beneficial effects: the disinfectant used in the disinfection method disclosed by the invention is highly toxic to pathogens, and a small amount of disinfectants can take a very good effect, the shrimp eggs are well tolerant to the disinfectant, the pathogens are killed without affecting the hatchability, the problems of low survival rate and poor quality in a hatching process is effectively solved, the disinfection step is simple, the operation is convenient, the practicability is high and good economic benefits are realized.

The invention discloses a disinfector used for shrimp eggs. The disinfector contains the following components in parts by weight: 3-8 parts of streptomyces globisporus, 4-7 parts of crab shell extracts, 5-10 parts of limonene, 1-3 parts of beta-phellandrene, 2-5 parts of caryophyllene and 3-6 parts of citric acid. The disinfector disclosed by the invention has the beneficial effects that the toxicity to pathogene is strong, and a small amount of disinfector can play a good disinfection role; the shrimp eggs have better tolerance to the disinfector, the usage range of the disinfector is enlarged, and the disinfection time of the disinfector is prolonged; a preparation method of the disinfector is simple, the used time is short, the quality stability of the disinfector is good, the cost is low and the application effect is good, also the preparation method has good economical benefits, and is an environment-friendly process.

The invention discloses a biocontrol streptomyces for preventing and treating camellia oleifera diseases and its application. A bacterial strain of the invention is streptomyces globisporus supsp.globisporus CSUFTAC0906002 and the preservation number is CCTCC M 2011012. The method comprises that: mixing fermentation broth of the bacterial strain with rice bran, naturally air-drying and grinding to produce raw powder; adding 40wt% of raw powder, 38wt% of kaolin, 10wt% of sodium lignosulfonate, 7wt% of SDS and 5 wt% of humic acid for preparing inocula; diluting inocula for directly applied to prevent and treat camellia oleifera diseases by a sprinkling and pouring root mode. The bacterial strain has preventive and therapeutic effect on camellia oleifera diseases, and has an antagonistic action on a plurality of pathogenic fungi. The bacterial strain has the advantages of various action modes and rapid breeding speed to the pathogen, especially has strong inhibiting effect on camellia anthracnose. The invention has important significance on preventing and treating camellia anthracnose, which has good application prospect for developing.

The invention discloses a compound microbial source pesticide for effectively preventing and treating diseases of oil-tea trees and an application method, and in particular relates to a microbial pesticide prepared by actinomycetes globisporus and streptomyces globisporus subspecies CSUFTAC0906002 (preserving number: CCTCC NO: M2011012) and bacterial bacillus brevis Z26 (preserving number: CCTCC NO: M2011013) compounded with other assistants. The invention belongs to the technical field of forest protection. The compound microbial source pesticide can effectively inhibit growth and reproduction of main pathogenic bacteria of the oil-tea trees, and meanwhile, the main components are good in safety to the environment and have good development application prospect.