The invention belongs to the field of biotechnologies, in particular to an axenic
germination and culture method of roegneria kamoji
plant seeds. The method comprises the following steps: seed preprocessing: selecting plump seeds which are not damaged by diseases and insects, washing the seeds by
tap water for 15-20min, and
steeping the seeds in
sterile water at 2-4 DEG C for 12-30h; then explantsterilizing: peeling off the flower
glume of the preprocessed seeds, sterilizing by
alcohol with the alcoholic strength of 75 percent for 25-40s, washing by
sterile water for 3 times, sterilizing by 0.1 percent of mercuric
chloride solution for 1-4min, washing by
sterile water for 5 times again, then sucking the
moisture on the surfaces of the seeds by sterile
filter paper, and then transferring the seeds to a
solid culture medium which is an MS;1 / 2MS culture medium; firstly, culturing in a light-proof
enclosure, then adopting lighting culture when buds grow to be 1-2 cm in height, and then culturing sound seedlings, namely, the seedlings are transferred to a 1 / 2MS culture medium when the seedlings grows to be 8-10 cm in height so that the height of the seedlings is kept to be 10+ / -2 cm. The invention improves the
germination ability of roegneria kamoji plants, increases the
germination index from 14.48 to 42.06 and obtains uniform sterile seedlings, thereby satisfying the requirements of seed planting and genetic breeding experiments of roegneria kamoji plants.