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Method for rapid propagation of potato seedlings or induction of test-tube tubers using chlorine dioxide disinfection medium

A chlorine dioxide and potato technology, which is applied in the field of plant tissue culture, can solve the problems of lack of systematic research, residual disinfectant substances, high price, etc., and achieve the effects of simplifying disinfection procedures, improving production levels, and reducing energy costs

Active Publication Date: 2019-03-12
甘肃亚盛薯业集团有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are problems of high price, residual disinfectant substances after sterilization, and lack of systematic research

Method used

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  • Method for rapid propagation of potato seedlings or induction of test-tube tubers using chlorine dioxide disinfection medium
  • Method for rapid propagation of potato seedlings or induction of test-tube tubers using chlorine dioxide disinfection medium
  • Method for rapid propagation of potato seedlings or induction of test-tube tubers using chlorine dioxide disinfection medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation of embodiment one chlorine dioxide disinfection medium

[0024] According to the chemical equation 5NaClO+4HCl=4ClO 2 +5NaCl+2H 2O, the rapid preparation of chlorine dioxide was achieved by the reaction of sodium chlorite and food-grade hydrochloric acid.

[0025] Weigh 1.5 g of sodium chlorite into a brown bottle, add 50 mL of distilled water, after it is completely dissolved, add 5 mL of food grade hydrochloric acid, react for 10 minutes, then add 450 mL of distilled water, mix well to obtain 1000 mg / L Aqueous chlorine dioxide solution. Dilute with distilled water to the desired concentration of 1-150 mg / L before use.

[0026] Preparation of virus-free potato seedling rapid propagation medium: Weigh an appropriate amount of plant MS medium components and place them in 1-150 mg / L chlorine dioxide aqueous solution with 60% of the volume of the prepared medium, add 40 g / L sucrose, and add 40 g / L sucrose at a rate of 100 per minute. Stir and sterilize ...

Embodiment 2

[0029] Embodiment Chlorine dioxide disinfection medium cultivates virus-free potato seedlings

[0030] The tested potato varieties included Hua 1, Zhong 5 and A-R-I. Cut the virus-free potato seedlings into stem segments with stem nodes about 1 cm long in the ultra-clean workbench, and insert the virus-free potato seedling rapid propagation medium prepared in Example 1 into the lower end of the morphology. The medium with the same composition was prepared by high temperature and high pressure sterilization as a control. Cultivate at 25±1°C, light 14 hours / day, light intensity 6000 Lx. After 20 days, the potato seedlings can grow to 10 cm long, and the growth is no different from the high-temperature and high-pressure sterilization control medium, and the seedlings have more branches than the control ( figure 1 ). Too low chlorine dioxide concentration could not achieve thorough disinfection of medium and potato materials, resulting in contamination of part of the medium and...

Embodiment 5

[0031] Example 3 Chlorine Dioxide Disinfection Medium Induces Potato Test Tube Tuber

[0032] Hua 1, Zhong 5 and A-R-I were used as the tested potato varieties. Cut the virus-free potato seedlings into stem segments with stem nodes about 1 cm long in the ultra-clean workbench, and insert the virus-free potato tuber tuber tuber induction medium prepared in Example 1 at the lower end of the morphology. The culture conditions are light 10 hours / day, temperature 25±1°C in light, light intensity 6000 Lx, and temperature 19±1°C in dark. After 30 days, tuber tubers began to form ( figure 2 ). All three tested varieties successfully induced test-tube tubers, and the induction rate of test-tube tubers reached more than 80% ( image 3 , Figure 4 ). After 50 days, the test tube tubers were harvested and preserved ( Figure 5 , Figure 6 ).

[0033] In this example, 1-150 mg / L liquid chlorine dioxide can completely disinfect the test potato culture medium and successfully induce...

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Abstract

The invention discloses a method for adopting a chlorine dioxide sterilization culture medium for potato seedling fast-propagation or potato microtuber induction. The method is characterized in that under room temperature, liquid chlorine dioxide is used as a sanitizer, and a sterile virus-free potato seedling fast-propagation culture medium or a virus-free potato microtuber induction culture medium is quickly obtained for virus-free potato seedling fast-propagation or potato microtuber induction. The culture medium can be efficiently sterilized without a high-temperature and high-pressure sterilization pot, a culture container material is not specially required, a culture medium sterilization procedure is simplified, the sterilization time is prolonged, and the energy consumption cost is greatly reduced, so the production cost of virus-free potato breeder apostrophe s seeds is greatly reduced, large-scale application of virus-free potato seed sources in production is promoted favorably, and the potato production level of China is increased.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, and more specifically relates to a new method for the rapid propagation of potato seedlings or the induction of test-tube tubers by a chlorine dioxide disinfection medium. Background technique [0002] Potato is a dicotyledonous plant belonging to Solanaceae Solanaceae, a multi-purpose crop for food, vegetables, and feed. It has wide adaptability to habitats and is suitable for environmental conditions where other major crops are difficult to grow. It is grown in 155 countries around the world. At the beginning of 2016, the Ministry of Agriculture of my country issued the "Guiding Opinions on Promoting the Development of the Potato Industry", proposing that by 2020, the potato planting area will expand to more than 100 million mu, the proportion of varieties suitable for staple food processing will reach 30%, and staple food consumption will account for the total consumption of potato...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 段永波赵丰兰滕井通盛玮张爱民薛建平
Owner 甘肃亚盛薯业集团有限责任公司
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