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Disinfectant for shrimp eggs

A disinfectant and shrimp egg technology, which is applied in antifungal agents, medical raw materials derived from bacteria, and medical raw materials derived from crustaceans, can solve the problems of reducing the activity of shrimp eggs, achieve extended disinfection time, simple preparation method, and eliminate pathogens. toxic effect

Active Publication Date: 2020-09-22
ZHEJIANG INST OF FRESH WATER FISHERIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The prior art, such as the Chinese invention patent with the authorized announcement number CN 103960182 B, discloses a method of using shrimp eggs to culture in vitro to establish a family of white prawns. Soaking and disinfection in potassium sea water solution reduces the impact of pathogens, but potassium permanganate also greatly reduces the activity of shrimp eggs

Method used

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Embodiment 1

[0013] A disinfectant for shrimp eggs. The ingredients and parts by weight of the disinfectant are: 3 parts of Streptomyces coccidioides fermentation broth, 4 parts of crab shell extract, 5 parts of limonene, 1 part of β-phellandrene, and 2 parts of caryophyllene parts, 3 parts of citric acid. The preparation method of the disinfectant comprises: first, the preparation of Streptomyces coccidioides fermentation broth: light the alcohol lamp, sterilize the inoculation loop by flame burning, extend the sterilized inoculation loop into the strain tube, and first put the loop into contact with the tube wall, to achieve the purpose of cooling, and then pick 1 part of bacterial lawn, withdraw the inoculation loop from the strain tube, quickly extend into 9 parts of sterile normal saline to obtain a suspension of bacteria, add 4 parts of active short peptide to the suspension, Then it was inoculated into 40 parts of Cha's culture medium, cultured on a constant temperature shaker at 20...

Embodiment 2

[0015] A disinfectant for shrimp eggs, the composition and parts by weight of the disinfectant are: 8 parts of Streptomyces coccidioides fermentation broth, 7 parts of crab shell extract, 10 parts of limonene, 3 parts of β-phellandrene, 5 parts of caryophyllene parts, 6 parts of citric acid. The preparation method of this disinfectant comprises:

[0016] 1) Preparation of Streptomyces coccidioides fermentation broth: Take 3 parts of Streptomyces coccidioides, add 12 parts of sterile normal saline to obtain a suspension of bacteria, add 6 parts of active short peptides to the suspension, and then inoculate 50 parts of Streptomyces coccidioids Streptomyces culture medium, cultured on a constant temperature shaker at 23°C and 175r / min for 5 days, centrifuged, and the supernatant was taken to obtain Streptomyces fermentation broth. Wherein, the composition of Cha's culture solution and its parts by weight are: 7 parts of sucrose, 3 parts of FeSO 4 , 2 parts NaNO 3 , 3 parts K ...

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Abstract

The invention discloses a disinfector used for shrimp eggs. The disinfector contains the following components in parts by weight: 3-8 parts of streptomyces globisporus, 4-7 parts of crab shell extracts, 5-10 parts of limonene, 1-3 parts of beta-phellandrene, 2-5 parts of caryophyllene and 3-6 parts of citric acid. The disinfector disclosed by the invention has the beneficial effects that the toxicity to pathogene is strong, and a small amount of disinfector can play a good disinfection role; the shrimp eggs have better tolerance to the disinfector, the usage range of the disinfector is enlarged, and the disinfection time of the disinfector is prolonged; a preparation method of the disinfector is simple, the used time is short, the quality stability of the disinfector is good, the cost is low and the application effect is good, also the preparation method has good economical benefits, and is an environment-friendly process.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a disinfectant for shrimp eggs. Background technique [0002] In recent years, my country's shrimp farming industry has developed rapidly, and a large number of products are exported. The farming areas are all over my country's Jiangsu, Shanghai, Zhejiang, Fujian, Jiangxi, Guangdong, Hunan, Hubei, Sichuan, Hebei, Henan, Shandong and other places. However, due to the outbreak of shrimp diseases , Seriously damaged the shrimp farming industry and related industries. Studies have shown that pathogens can cause disease outbreaks by invading shrimp eggs and spreading rapidly in fry ponds. [0003] The prior art, such as the Chinese invention patent with the authorized announcement number CN 103960182 B, discloses a method of using shrimp eggs to culture in vitro to establish a family of white prawns. Soaking and disinfection in potassium sea water solution reduces the impact of p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/74A61P31/04A61P31/10A61K35/612A61K31/015A61K31/194
CPCA61K31/015A61K31/194A61K35/612A61K35/74A61K2300/00
Inventor 黄振远李喜莲顾志敏郭建林
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
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