Strain of streptomycete for preventing sheath blight of rice

A technology for rice sheath blight and rice blast, which can be applied in the directions of bacteria, botanical equipment and methods, and microbial determination/inspection, and can solve human health and environmental hazards, limited genetic engineering, and lack of high sheath blight resistance genes. and other problems, to achieve the effect of solving pests and diseases, strong antagonism and good control effect

Inactive Publication Date: 2004-08-11
成都川农邑邦农业发展有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of high sheath blight resistance genes in the currently promoted rice varieties, and the use of disease-resistant varieties has not yet achieved satisfactory disease control effects. In addition, with the large-scale promotion of dwarf varieties and hybrid rice and the impact of rice field management measures , so the occurrence area of ​​rice sheath blight is expanding year by year, resulting in a loss rate of more than 10% of my country's annual rice output
At present, in actual production, Jinggangmycin is mainly used to prevent and control the disease. Although its effect is good, it has caused the sheath blight bacteria to develop drug resistance due to its singleness and long-term use.
In the Sichuan rice area, rice blast is the most harmful in the physiological race ZB15, but there is still a long way to clone the blast resistance gene from rice plants. Therefore, the genetic engineering of rice blast resistant rice limited, and general chemical pesticides will cause great harm to human health and the environment
As we all know, Streptomyces fungi are important fungi that produce small molecule antibiotic substances, but there are no related reports on the research and utilization of Streptomyces coccidioides in the use of microorganisms to prevent and control crop diseases and insect pests

Method used

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  • Strain of streptomycete for preventing sheath blight of rice
  • Strain of streptomycete for preventing sheath blight of rice
  • Strain of streptomycete for preventing sheath blight of rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: the screening method of H50 bacterial strain

[0039] Pick an appropriate amount of soil collected from various test sites in Wenjiang, Sichuan, and place it in a 250ml Erlenmeyer flask filled with sterile water, and cultivate it at 120 rpm at 25°C for 12 hours, then draw 1ml of the bacterial solution, and use Concentration gradient method is applied to PDA plates (200 grams of potatoes, 10-20 grams of sucrose, 17-20 grams of agar, 1000 ml of water), and cultured in a constant temperature incubator at 26-28 °C. After 48 hours, single colonies with different culture characteristics were picked and purified repeatedly for testing. First use the dot connection method for primary selection, and then use the plate confrontation culture method for the strains that have inhibition zones against rice sheath blight and rice blast pathogens.

Embodiment 2

[0040] Embodiment 2: Antibiotic research of antagonistic bacteria

[0041] A. Determination of the antibacterial rate of antagonistic bacteria on sheath blight bacteria and rice blast pathogens: put the mycelia blocks of the same age in the bacterial fermentation broth passed through the microporous membrane and soak for 5 minutes, and then place them on PDA and YPTG by spot connection method Cultivate on the plate at 28°C, control with clean water, and calculate the antibacterial rate.

[0042] By measuring the growth of the sheath blight mycelia block containing the supernatant of the antagonistic substances collected after shaking culture in the PAD liquid medium, the bacteriostatic rate of the H50 strain was 67.3%; on the YPTG medium, The antibacterial rate of H50 strain reached 62.5%.

[0043] B. Determination of the teratogenic effect of antagonistic bacteria on sheath blight mycelia and rice blast: the antagonistic bacteria are inoculated with the confrontation method,...

Embodiment 3

[0050] Embodiment 3: Identification of bacterial strain

[0051] A. Morphological characteristics: After culturing on peptone medium and beef extract agar at 30°C for 2 to 4 days, observe the morphology of bacteria with electron microscope and optical microscope and take pictures.

[0052] B. Culture characteristics: Gaoshi Synthetic Agar No. 1, Glucose Aspartate Agar, Sucrose Nitrate Agar, Starch Ammonium Salt Agar, Inorganic Salt Starch Agar Yeast Refined Malt Agar, Oat Flour Agar, Potato Extract Agar Medium 30℃ After 2 to 4 days of culture, observe the colony shape and cell color.

[0053] C. Chemical classification: analysis of chemical components of cell wall The analysis of amino acids and glycoforms of the whole cell hydrolyzate was carried out by fast thin-plate chromatography (TCL) improved by Hasegawa.

[0054] D. Physiological and biochemical experiments: Refer to the content of "Bergeys Manual of Sustematic Bacteriology" VO1.II to conduct physiological and biochem...

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Abstract

A streptomyces globisporus H50 (CCTCC No.M203034) for preventing and eliminating the hypochnus and blast of rice is obtained from soil by screening with PAD and YPTG plate method. Its advantage is high effect (more than 65% for its bacterium inhibiting rate).

Description

Technical field: [0001] The present invention relates to a bacterial strain of the genus Streptomyces, in particular to a screening technique utilizing Streptomyces, through the primary screening of PAD and YPTG plate application point connection method, to obtain the pathogenic bacteria of rice sheath blight and rice blast that have strong antagonism Acting Streptomyces strains. Background technique: [0002] Rice sheath blight (Thanatephorus cucumeris) and rice blast (Pyricularia) are two major diseases in rice fields in my country. Since 1987, the occurrence of rice sheath blight and rice blast has become serious every year due to the high temperature and high humidity in the rice fields of Sichuan. Due to the lack of high sheath blight resistance genes in the currently promoted rice varieties, and the use of disease-resistant varieties has not yet achieved satisfactory disease control effects. In addition, with the large-scale promotion of dwarf varieties and hybrid ric...

Claims

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Application Information

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IPC IPC(8): A01N63/00C12N1/20C12Q1/04
Inventor 郑爱萍李平
Owner 成都川农邑邦农业发展有限公司
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