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Bio-control streptomyces globisporus strain and application in prevention and control of penicilliosis of citrus

A technology for Streptomyces coccidioides and citrus penicillium, which is applied in the field of agricultural microorganisms to achieve the effects of reducing toxic effects and enhancing practicability

Inactive Publication Date: 2010-09-29
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the control of citrus storage diseases by volatile substances produced by Streptomyces

Method used

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  • Bio-control streptomyces globisporus strain and application in prevention and control of penicilliosis of citrus
  • Bio-control streptomyces globisporus strain and application in prevention and control of penicilliosis of citrus
  • Bio-control streptomyces globisporus strain and application in prevention and control of penicilliosis of citrus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Isolation, screening and fermentation culture of Streptomyces coccidioides JK-1

[0029] 1. Separation and screening process

[0030] The microorganism of the present invention was isolated on a PDA plate naturally contaminated by the Key Laboratory of Crop Disease Detection and Safety Control of Hubei Province, Huazhong Agricultural University, Wuhan City, Hubei Province, to obtain a strain of Streptomyces with strong antagonistic activity. The isolation and purification is a conventional method. Refer to the literature :Fang Zhongda (1998), the method is to pick Streptomyces colonies from naturally contaminated PDA plates, dilute with 5ml sterile water, dip a small amount of diluent with an inoculation needle and draw lines on the PDA plate, select the desired The characteristic single colony, after 3 times of the same streaking culture, finally obtained pure Streptomyces strain. The applicant named it JK-1. Refer to the Streptomyces identification manual (Act...

Embodiment 2

[0037] Example 2: Preliminary Identification of Volatile Substances Produced by Streptomyces Sphaeroides JK-1

[0038] Take 40g of the wheat kernel culture of Streptomyces sphaeroides JK-1 cultured for 14 days (without the addition of Streptomyces sphaeroides JK-1, the sterilized wheat kernel culture cultured for 14 days as the control, the same below), and put 40g into the headspace microextraction bottle Then add 50mL of saturated NaCl solution and magnetically stir, equilibrate at 60°C for 40 minutes, and recover the extraction head. Pull the extraction head out of the extraction flask and immediately insert it into the gas chromatography injection hole. Desorb at 280°C for 5 minutes for gas chromatography / mass spectrometry. (GC / MS) analysis, gas chromatogram such as figure 1 , 2 Shown. The obtained mass spectra of each gas component are compared with the NIST database (Agilent 7890A, Agilent Company, USA) to identify the components of volatile substances. 35 compounds were i...

Embodiment 3

[0039] Example 3: Inhibition of volatile substances produced by Streptomyces sphaeroides JK-1 on mycelial growth and sporulation of Penicillium citrus

[0040] Place four small petri dishes with a diameter of 60 mm and a height of 15 mm without a lid in a large petri dish with a diameter of 150 mm and a height of 30 mm; three of the petri dishes contain about 5 mL of PDA medium (Fang Zhongda, 1998) and inoculate with a diameter of 5 mm The citrus penicillium hyphae block, another small petri dish is placed the wheat grain culture of Streptomyces coccidius JK-1, and then the large petri dish is sealed with parafilm. Set up 5 different treatments respectively, the amount of the grain culture of Streptomyces sphaeroides JK-1 was 0g, 1.875g, 3.75g, 7.5g, 15g, 30g, respectively. After culturing for 5 days at 25°C, observe and measure the size of the colony diameter in each small petri dish and the amount of spores produced in each petri dish. The result is figure 1 , 2 Shown. The vo...

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Abstract

The invention belongs to the technical field of agro-microbiology, and in particular relates to the isolation and identification of a biological control streptomyces globisporus strain and the application of the biological control streptomyces globisporus strain in prevention and control of the penicilliosis of citrus. In the invention, the streptomyces globisporus strain JK-1 capable of producing antifungal volatile substances is obtained, is streptomyces globisporus and is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO: M2010093. The invention also provides microbial agents prepared from the streptomyces globisporus strain JK-1. The volatile substances produced by the streptomyces globisporus strain JK-1 are utilized to prevent and control the penicilliosis of the citrus in preservation and freshness, which indicates that microorganisms and the agents thereof belong to novel biological control agents.

Description

Technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and specifically relates to a strain of Streptomyces sphaeroides, in particular to the isolation and identification of a strain of Streptomyces sphaeroides that can produce anti-fungal volatile substances and its application in the prevention and treatment of citrus penicillium. Background technique [0002] Citrus is one of the main fruit trees in the world, with an annual output of more than 65 million tons. Citrus fruits are prone to mildew and spoilage caused by fungi or bacteria during storage and transportation after harvest, which will directly cause a decline in citrus yield and storage quality, resulting in great economic losses. Penicillium citrus is one of the most serious diseases during the storage period of citrus. According to reports, the annual loss of citrus yield caused by Penicillium citrus in China is 10-30%, and can reach 30-50% in severe cases. At present, t...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/465
Inventor 黄俊斌李其利郑露
Owner HUAZHONG AGRI UNIV
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