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Bio-control streptomyces globisporus strain and application in prevention and control of penicilliosis of citrus

A technology for streptomyces coccidioides and citrus penicillium, applied in the field of agricultural microorganisms, to achieve the effect of reducing toxicity and enhancing practicability

Inactive Publication Date: 2011-08-31
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the control of citrus storage diseases by volatile substances produced by Streptomyces

Method used

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  • Bio-control streptomyces globisporus strain and application in prevention and control of penicilliosis of citrus
  • Bio-control streptomyces globisporus strain and application in prevention and control of penicilliosis of citrus
  • Bio-control streptomyces globisporus strain and application in prevention and control of penicilliosis of citrus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Isolation, screening and fermentation of Streptomyces coccidioides JK-1

[0029] 1. Separation and screening process

[0030] The microorganism of the present invention is obtained by separating and obtaining a Streptomyces strain with strong antagonistic activity on the naturally polluted PDA plate of Hubei Provincial Key Laboratory of Crop Disease Detection and Safety Control, Huazhong Agricultural University, Wuhan City, Hubei Province. The separation and purification is a conventional method, referring to the literature : Fang Zhongda (1998), the method is to pick Streptomyces colony from the PDA plate of natural pollution, after diluting with 5ml sterile water, dip a small amount of diluent with the inoculation needle and draw a line on the PDA plate, select the required Characteristic single colony, after 3 identical streak cultures, finally obtained pure Streptomyces strains. The applicant named it JK-1. Referring to the Streptomyces identification m...

Embodiment 2

[0037] Example 2: Preliminary identification of volatile components produced by Streptomyces coccidioides JK-1

[0038] Put 40 g of Streptomyces coccidioides JK-1 kernel culture cultured for 14 days (without Streptomyces coccoides JK-1, sterilized wheat kernel culture cultured for 14 days as a control, the same below) into a headspace microextraction bottle Then add 50mL of saturated NaCl solution with magnetic stirring, recover the extraction head after equilibrating at 60°C for 40min, pull out the extraction head from the extraction bottle and insert it into the gas chromatography injection hole immediately, desorb at 280°C for 5min for gas chromatography / mass spectrometry (GC / MS) analysis, gas chromatogram such as figure 1 , 2 shown. The obtained mass spectrograms of each gas component were compared with the NIST database (Agilent7890A, Agilent Corporation, USA), so as to identify the components of the volatile substances. Thirty-five compounds were identified from the c...

Embodiment 3

[0039] Example 3: Inhibition of the volatile substances produced by Streptomyces coccidioides JK-1 on the growth of Penicillium citri mycelium and the formation of spores

[0040] Put four small petri dishes with a diameter of 60 mm and a height of 15 mm without a cover in a large petri dish with a diameter of 150 mm and a height of 30 mm; among them, three small petri dishes are filled with about 5 mL of PDA medium (Fang Zhongda, 1998) and inoculated with a diameter of 5 mm citrus mycelia block, another small petri dish was placed the wheat grain culture of Streptomyces coccidioides JK-1, and then the big petri dish was sealed with parafilm. Five different treatments were set up, and the amount of Streptomyces coccidioides JK-1 kernel culture was 0g, 1.875g, 3.75g, 7.5g, 15g, 30g respectively. After culturing for 5 days under the condition of 25° C., observe and measure the size of the colony diameter in each small petri dish and the amount of spores produced by each petri di...

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Abstract

The invention belongs to the technical field of agro-microbiology, and in particular relates to the isolation and identification of a biological control streptomyces globisporus strain and the application of the biological control streptomyces globisporus strain in prevention and control of the penicilliosis of citrus. In the invention, the streptomyces globisporus strain JK-1 capable of producing antifungal volatile substances is obtained, is streptomyces globisporus and is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO: M2010093. The invention also provides microbial agents prepared from the streptomyces globisporus strain JK-1. The volatile substances produced by the streptomyces globisporus strain JK-1 are utilized to prevent and control the penicilliosis of the citrus in preservation and freshness, which indicates that microorganisms and the agents thereof belong to novel biological control agents.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a strain of Streptomyces coccidioides, in particular to the isolation and identification of a strain of Streptomyces coccidioides capable of producing antifungal volatile substances and its application in the prevention and treatment of citrus penicillium. Background technique [0002] Citrus is one of the main fruit trees in the world, with an annual production of more than 65 million tons. Citrus fruits are prone to mildew and deterioration caused by fungi or bacteria during storage and transportation after harvest, which will directly cause a decrease in citrus yield and storage quality, resulting in great economic losses. Penicillium citrus is one of the most serious diseases during the storage period of citrus. It is reported that in China, the annual loss of citrus yield caused by Penicillium citrus is 10-30%, and it can reach 30-50% in sever...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01P3/00A01N63/02C12N1/20C12R1/465
Inventor 黄俊斌李其利郑露
Owner HUAZHONG AGRI UNIV
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