Chromium-rich yeast culture and fermentation process thereof
A technology of chromium-enriched yeast and fermentation technology, which is applied in fermentation, microorganisms, animal feed, etc., can solve the problems of limited beneficial ingredients, high production cost, and large investment amount, and achieve the effect of improving the biological value of products
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Embodiment 1
[0020] 1. Strain: Candida tropicalis AS2.637 purchased from Guangzhou Institute of Microbiology
[0021] 2. Medium: glucose 2%, peptone 1%, yeast extract 0.5%, pH=6.8, inorganic chromium (derived from chromium trichloride) 0.2%, culture for 24h, confirm that the strain can maintain good growth in the above-mentioned medium .
Embodiment 2
[0023] 1. Strain: Same as Example 1.
[0024] 2. Inclined medium and culture conditions:
[0025] Glucose 2%, peptone 1%, yeast extract 0.5%, agar 2%. pH=6.8, sterilization at 105°C for 18 minutes. The test tube strain was expanded to a petri dish and placed in a 30°C constant temperature incubator for 24 hours.
Embodiment 3
[0027] 1. The strain is the same as in Example 1.
[0028] 2. Inclined cultivation is the same as in Example 2.
[0029] 3. Shake flask seed culture medium and culture conditions:
[0030] Glucose 2%, peptone 1%, yeast extract 0.5%. Start pH=6.8, sterilize at 105°C for 18 minutes. . Connect the culture dish strains to the shake flask, put them in a 30°C constant temperature shaker, shake culture at 200r / min for 24h.
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