Composite biological antimicrobial for preventing wilt and greensickness of cotton
A biocontrol fungicide and Fusarium wilt technology, applied in the direction of biocide, bactericide, biocide, etc., can solve the problems of no public name anti-cotton Fusarium wilt control effect, etc., to reduce the dead seedling rate and increase the yield rate The effect of improving and increasing production
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Embodiment 1
[0018] The preparation of the composite biocontrol bacterium powder of embodiment 1 anti-cotton fusarium wilt
[0019] The composite biocontrol agent for resisting cotton fusarium wilt consists of agent A, agent B, agent C and agent D. Bacterial agent A, bacterial agent B, bacterial agent C and bacterial agent D are prepared according to the following method:
[0020] Preparation of Potato Dextrose Agar Medium (PDA): Weigh 200g of peeled potatoes, cut into pieces and boil for 30min, then filter the juice with gauze, add 20.0g of glucose and 20.0g of agar, add water to 1000mL after melting, and distribute to Ten 150mL Erlenmeyer flasks can be sterilized at 121°C for 30 minutes.
[0021] Candida tropicalis was cultivated in the above PDA medium at 28°C for 3 days with shaking at 200 r / min, and all the fermentation broth was collected and dried under low-temperature negative pressure as bacterial agent A.
[0022] Streptomyces microflavus was cultivated in the above PDA medium ...
Embodiment 2
[0026] The preparation of the composite biocontrol bacterium powder of embodiment 2 anti-cotton fusarium wilt
[0027] The compound biocontrol fungicides used for resisting cotton fusarium wilt include fungicide A, fungicide B, fungicide C and fungicide D. Wherein bacterial agent A, bacterial agent B, bacterial agent C and microbial agent D are prepared according to the following method:
[0028] Preparation of Potato Dextrose Agar Medium (PDA): Weigh 200g of peeled potatoes, cut into pieces and boil for 30min, then filter the juice with gauze, add 20.0g of glucose and 20.0g of agar, add water to 1000mL after melting, and distribute to Ten 150mL Erlenmeyer flasks can be sterilized at 121°C for 30 minutes.
[0029] Candida tropicalis was cultivated in the above PDA medium at 28°C for 3 days with shaking at 200 r / min, and all the fermentation broth was collected and dried under low-temperature negative pressure as bacterial agent A.
[0030] Streptomyces microflavus was cultiv...
Embodiment 3
[0034] The preparation of the composite biocontrol bacteria water agent of embodiment 3 anti-cotton fusarium wilt
[0035] The composite biocontrol agent for resisting cotton fusarium wilt consists of agent A, agent B, agent C and agent D. Bacterial agent A, bacterial agent B, bacterial agent C and bacterial agent D are prepared according to the following method:
[0036] Preparation of Potato Dextrose Agar Medium (PDA): Weigh 200g of peeled potatoes, cut into pieces and boil for 30min, then filter the juice with gauze, add 20.0g of glucose and 20.0g of agar, add water to 1000mL after melting, and distribute to Ten 150mL Erlenmeyer flasks can be sterilized at 121°C for 30 minutes.
[0037] Candida tropicalis was cultivated in the above PDA medium at 28° C. and shaken at 200 r / min for 3 days, and all the fermentation broth was collected as bacterial agent A.
[0038] Streptomyces microflavus was cultured in the above PDA medium at 28° C. and shaken at 200 r / min for 3 days, an...
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