Sea cucumber microecological water quality conditioning agent and method for preparing same
A water quality regulator and micro-ecological technology, which is applied in the field of aquaculture technology and microorganisms, can solve the problems of ineffective purification of aquaculture water, and achieve the effects of avoiding substrate corruption, significant purification effects, and stress relief
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Embodiment 1
[0042] 1: Prepare the seed solution of probiotics.
[0043] Bacillus subtilis (Bacillus subtilis) YB-1CGMCC No.5116, Bacillus cereus (Bacillus cereus) YB-2CGMCC No.5117 and Bacillus flexus (Bacillus flexus) XH-1CGMCCNo. 5115, after adding water respectively, the concentration of bacteria were made into 1×10 8 cfu / ml bacterial suspension, the above three bacterial suspensions are mixed according to the ratio of the number of bacteria 1:1:1 to obtain a mixed bacterial suspension, and the mixed bacterial suspension is inoculated in the fermentation broth with 3% of the volume of the fermentation broth , stir evenly for 1-2min, the fermentation condition is 30°C, 24h; prepare probiotic seed liquid.
[0044] The above-mentioned fermented liquid is mixed by corn flour: bean cake powder: water in a weight ratio of 1.5: 0.7: 100, and the pH is adjusted to 7.0-8.0;
[0045] 2: Liquid fermentation.
[0046] First inoculate the nutrient substance in the above-mentioned fermentation br...
Embodiment 2
[0055] 1: Prepare the seed solution of probiotics.
[0056] Bacillus subtilis (Bacillus subtilis) YB-1CGMCC No.5116, Bacillus cereus (Bacillus cereus) YB-2CGMCC No.5117 and Bacillus flexus (Bacillus flexus) XH-1 CGMCC that grew well on LB agar slant medium No.5115, after adding water respectively, the bacterial cell concentration is 1×10 8 cfu / ml bacterial suspension, the above three bacterial suspensions are mixed according to the ratio of the number of bacteria 1:1:1 to obtain a mixed bacterial suspension, and the mixed bacterial suspension is inoculated in the fermentation broth with 3% of the volume of the fermentation broth , stir evenly for 1-2min, the fermentation condition is 30°C, 24h; prepare probiotic seed liquid.
[0057] The above fermented liquid is mixed with corn flour: bean cake powder: water in a weight ratio of 3:1:100 to adjust the pH to 7.0-8.0;
[0058] 2: Liquid fermentation.
[0059] First inoculate the nutrient substance in the above-mentioned ferme...
Embodiment 3
[0068] 1: Prepare the seed solution of probiotics.
[0069] Bacillus subtilis (Bacillus subtilis) YB-1CGMCC No.5116, Bacillus cereus (Bacillus cereus) YB-2CGMCC No.5117 and Bacillus flexus (Bacillus flexus) XH-1 CGMCC that grew well on LB agar slant medium No.5115, after adding water respectively, the bacterial cell concentration is 1×10 8 cfu / ml bacterial suspension, the above three bacterial suspensions are mixed according to the ratio of the number of bacteria 1:1:1 to obtain a mixed bacterial suspension, and the mixed bacterial suspension is inoculated in the fermentation broth with 3% of the volume of the fermentation broth , stir evenly for 1-2min, the fermentation condition is 30°C, 24h; prepare probiotic seed liquid.
[0070] The above fermented liquid is mixed with corn flour: bean cake powder: water in a weight ratio of 3:1:100 to adjust the pH to 7.0-8.0;
[0071] 2: Liquid fermentation.
[0072] First inoculate the nutrient substance in the above-mentioned ferme...
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