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Sea cucumber microecological water quality conditioning agent and method for preparing same

A water quality regulator and micro-ecological technology, which is applied in the field of aquaculture technology and microorganisms, can solve the problems of ineffective purification of aquaculture water, and achieve the effects of avoiding substrate corruption, significant purification effects, and stress relief

Inactive Publication Date: 2012-08-01
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the micro-ecological water quality regulators used in aquaculture are limited to one or two types of probiotics to purify the aquaculture water body, and the purification effect on the aquaculture water body is not obvious

Method used

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  • Sea cucumber microecological water quality conditioning agent and method for preparing same
  • Sea cucumber microecological water quality conditioning agent and method for preparing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1: Prepare the seed solution of probiotics.

[0043] Bacillus subtilis (Bacillus subtilis) YB-1CGMCC No.5116, Bacillus cereus (Bacillus cereus) YB-2CGMCC No.5117 and Bacillus flexus (Bacillus flexus) XH-1CGMCCNo. 5115, after adding water respectively, the concentration of bacteria were made into 1×10 8 cfu / ml bacterial suspension, the above three bacterial suspensions are mixed according to the ratio of the number of bacteria 1:1:1 to obtain a mixed bacterial suspension, and the mixed bacterial suspension is inoculated in the fermentation broth with 3% of the volume of the fermentation broth , stir evenly for 1-2min, the fermentation condition is 30°C, 24h; prepare probiotic seed liquid.

[0044] The above-mentioned fermented liquid is mixed by corn flour: bean cake powder: water in a weight ratio of 1.5: 0.7: 100, and the pH is adjusted to 7.0-8.0;

[0045] 2: Liquid fermentation.

[0046] First inoculate the nutrient substance in the above-mentioned fermentation br...

Embodiment 2

[0055] 1: Prepare the seed solution of probiotics.

[0056] Bacillus subtilis (Bacillus subtilis) YB-1CGMCC No.5116, Bacillus cereus (Bacillus cereus) YB-2CGMCC No.5117 and Bacillus flexus (Bacillus flexus) XH-1 CGMCC that grew well on LB agar slant medium No.5115, after adding water respectively, the bacterial cell concentration is 1×10 8 cfu / ml bacterial suspension, the above three bacterial suspensions are mixed according to the ratio of the number of bacteria 1:1:1 to obtain a mixed bacterial suspension, and the mixed bacterial suspension is inoculated in the fermentation broth with 3% of the volume of the fermentation broth , stir evenly for 1-2min, the fermentation condition is 30°C, 24h; prepare probiotic seed liquid.

[0057] The above fermented liquid is mixed with corn flour: bean cake powder: water in a weight ratio of 3:1:100 to adjust the pH to 7.0-8.0;

[0058] 2: Liquid fermentation.

[0059] First inoculate the nutrient substance in the above-mentioned ferme...

Embodiment 3

[0068] 1: Prepare the seed solution of probiotics.

[0069] Bacillus subtilis (Bacillus subtilis) YB-1CGMCC No.5116, Bacillus cereus (Bacillus cereus) YB-2CGMCC No.5117 and Bacillus flexus (Bacillus flexus) XH-1 CGMCC that grew well on LB agar slant medium No.5115, after adding water respectively, the bacterial cell concentration is 1×10 8 cfu / ml bacterial suspension, the above three bacterial suspensions are mixed according to the ratio of the number of bacteria 1:1:1 to obtain a mixed bacterial suspension, and the mixed bacterial suspension is inoculated in the fermentation broth with 3% of the volume of the fermentation broth , stir evenly for 1-2min, the fermentation condition is 30°C, 24h; prepare probiotic seed liquid.

[0070] The above fermented liquid is mixed with corn flour: bean cake powder: water in a weight ratio of 3:1:100 to adjust the pH to 7.0-8.0;

[0071] 2: Liquid fermentation.

[0072] First inoculate the nutrient substance in the above-mentioned ferme...

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Abstract

A microecological water quality conditioning agent applicable to restoring of sea cucumber culture water body and a method for preparing the microecological water quality conditioning agent belong to the technical field of aquaculture and microorganisms. By aiming at physiological habits and culturing characteristics of sea cucumbers and applying modern biological technique, the microecological water quality conditioning agent is made of bacillus flexus CGMCC (China general microbiological culture collection center) No.5115, bacillus subtilis CGMCC No.5116 and bacillus cereus CGMCC No.5117, which are separated and sieved from a sea cucumber culturing environment and prepared by steps: using corn starch and soybean cake powder as main culturing materials, fermentating for 24-48h at 25-40 DEG C, and using zeolite and wheat bran as adsorption carriers for processing to obtain the microecological water quality conditioning agent. The microecological water quality conditioning agent is less susceptible to environment conditions, has remarkable purifying effect on sea cucumber culturing water body, and is capable of greatly reducing content of harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide and the like, deodorizing and effectively restraining pathogenic bacteria so as to prevent sea cucumber diseases and improve yield and quality of cultured sea cucumbers.

Description

technical field [0001] The invention relates to the fields of aquaculture technology and microorganism technology, in particular to the preparation of a composite micro-ecological water quality regulator. Background technique [0002] In recent years, sea cucumber farming has become one of the pillar industries of China's aquaculture industry. However, with the rapid development of modern intensive and large-scale farming of sea cucumbers, some serious problems have appeared, which have restricted the development of its industry. During the process of feeding a large amount of high-protein bait, a lot of uneaten residual bait, feces excrement and biological dead bodies are decomposed into a lot of harmful substances in the water body, which deteriorates the water quality and causes a large number of pathogenic bacteria to cause strong stress in sea cucumbers. In response, phenomena such as head shaking, body contraction and stiffness, viscera discharge, mouth swelling, and b...

Claims

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Application Information

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IPC IPC(8): C02F3/34A01K61/00
CPCY02A40/81
Inventor 徐永平李建光金礼吉汪将车鉴蒲红宇
Owner DALIAN UNIV OF TECH
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