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Bifidobacterium longum and applications thereof

A technology of bifidobacteria longum and bifidobacteria, which is applied in the field of microorganisms to achieve the effects of good growth and acid production, acid resistance, strong adhesion, and improving the propulsion rate of the small intestine.

Active Publication Date: 2018-04-10
上海华朴生命健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there have been researches at home and abroad involving bifidobacteria to relieve or treat constipation, but mainly in the form of mixed bacteria and synbiotics, and only a single strain is used for research. In addition, due to individual differences and differences between different strains difference, there is still a certain probability that bifidobacteria can relieve constipation

Method used

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  • Bifidobacterium longum and applications thereof
  • Bifidobacterium longum and applications thereof
  • Bifidobacterium longum and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Screening of Bifidobacterium longum CCFM 760

[0039] 1) Use a disposable sterile toilet to collect several fresh feces of a two-year-old girl in Binhu District, Wuxi City, Jiangsu Province, and place the fecal sample in a modified MRS liquid test tube with oligofructose as a carbon source, and place it in an anaerobic workstation ( N 2 :CO 2 :H 2 =80:10:10) within 12h of enrichment of Bifidobacterium strains;

[0040] 2) Perform gradient dilution with sterile normal saline, use a pipette to draw 100 μL of the sample with the corresponding dilution and spread it on a modified MRS solid plate containing 0.02g / mL calcium carbonate, and incubate it anaerobic for 24-48h;

[0041] 3) Pick a single colony with a calcium carbonate-dissolving circle and a single colony conforming to the basic morphology of lactic acid bacteria for streaking separation on the modified MRS solid medium, and repeat the streaking 3 times;

[0042] 4) Pick a single colony and transfer it to the l...

Embodiment 2

[0046] Example 2: Preparation of Bifidobacterium longum CCFM760 bacterial suspension

[0047] Experimental method: Inoculate the bacterial solution after 3 generations of activation into 1L modified MRS medium at an inoculum of 2%, shake and mix well, and then anaerobic culture at 37°C for 20h. Centrifuge at 5000 rpm and 4°C for 15 minutes, discard the supernatant, wash twice with sterile saline, centrifuge under the same conditions, and resuspend with 15 mL of sterile 30% sucrose water. After shaking evenly, the number of viable bacteria was measured by the plate pouring method and stored in the refrigerator at -80°C for 1 week. Among them, the modified MRS medium formula is: beef extract 10g; tryptone 10g; yeast powder 5g; glucose 20g; anhydrous sodium acetate 5g; MgSO 4 ·7H 2 O 0.1g; MnSO 4 ·H 2 O 0.05g; Diammonium hydrogen citrate 2g; K 2 HPO 4 ·3H 2 O 2.6g; Tween-801mL; L-cysteine ​​hydrochloride 1g; adjust the pH to 6.8±0.2; dilute to 1L. Sterilize at 115°C for 20 minutes....

Embodiment 3

[0049] Example 3: Relief effect of Bifidobacterium longum CCFM760 on constipation in mice

[0050] Experimental method: The CCFM760 bacterial suspension with the function of relieving constipation in Example 1 was used as the experimental group, and the Bifidobacterium animalis BB12 and phenolphthalein were used as positive controls to conduct comparative experiments on normal mice and constipated mice. By comparing the fecal water content of mice in each group, the time to first black stool, the rate of small intestine advancement, the colon tissue section and the content of constipation-related gastrointestinal peptides in serum, the effect of Bifidobacterium longum CCFM760 on mice constipation was evaluated.

[0051] Take 25 healthy BALB / c male mice (7 weeks old) weighing 22-26g and randomly divide them into 5 groups (normal control group, constipation model group, CCFM760 experimental group, BB12 control group, phenolphthalein control group), each group There are 5 mice. The e...

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Abstract

The invention discloses bifidobacterium longum and applications thereof, and belongs to the technical field of microorganisms. The bifidobacterium longum is deposited in the China General Microbiological Culture Collection Center in Institute of Microbiology, Chinese Academy of Sciences at number 3, No.1 yard, Beichenxi road, Chaoyang district, Beijing on December 7, 2017, and the accession numberis CGMCC NO.15032. The bifidobacterium longum has good growth, acid production, and acid resistant properties, and high adhesive capacity, can significantly increase the water content of faeces and the pushing rate of small intestine of mice with constipation, shortens the first black stool evacuation time, relieves inflammation of colonic mucosas, and regulates contents of gastrointestinal peptides related to constipation in serum. A constipation relieving effect of the bifidobacterium longum is equivalent to that of bifidobacterium animalis BB12, but an effect of the bifidobacterium longumon the pushing rate of small intestine is superior to those of bifidobacterium animalis BB12 and phenolphthalein. Accordingly, the bifidobacterium longum strain CCFM760 can be widely applied in various foods or medicine matrixes.

Description

Technical field [0001] The invention relates to a strain of Bifidobacterium longum and its application, and belongs to the technical field of microorganisms. Background technique [0002] The prevalence of constipation has risen sharply with age. Investigations have found that the proportion of constipation among the elderly in Western countries is 40% and that in nursing homes is as high as 70%; the situation in my country is slightly better, with 11.9% of elderly constipation patients and 45.53 in nursing homes. %. Long-term difficulty in defecation can induce or aggravate other diseases such as hemorrhoids, enlarged prostate, myocardial infarction or cerebrovascular disease. In severe cases, it can even be life-threatening and become a public health problem. [0003] The causes of constipation include genetic factors, mental and psychological factors, dietary factors, abnormal bowel kinetics, abnormal hormones and neurotransmitters and other regulatory factors, and imbalance of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/745A61P1/10A23L33/135C12R1/01
CPCA61K35/745A61K2035/115A23L33/135C12N1/205C12R2001/01
Inventor 陈卫张秋香应聪萍张灏赵建新田丰伟王刚翟齐啸于雷雷
Owner 上海华朴生命健康科技有限公司
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